FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884135
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTTATAGTGCTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGGCGATGGTGAGAGTGAATTCTGTC40850.4620335129816148No Hit
CTATAACGGTTACCCCATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT37070.41927986110718385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33030.37358548185514656No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA32840.37143648877151114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24160.27326143631911415No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22930.2593495337250533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC22750.2573136455405566No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTGGCCGTGGTAGAAACAAAGCTAATGG21710.24555073603013114No Hit
GTCTTAGAAACTGAGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGCCAGC20550.23243056773004123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19650.22225112680755768No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19060.215577937758374No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18850.21320273487646116No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA18300.20698196542383232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17570.19872530778670677No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17530.19827288819015196No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17180.19431421672029722No Hit
GAATTCACTCTCACCATCGCCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAGCACTATAACGGTTAC16720.18911139135991675No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16170.18289062190728791No Hit
CCTTCCACCCTGTCTGCATCTATTGGAGACAGAGTCATCATCACTTGCCG15940.18028920922709765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15760.17825332104260094No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACCTCAGTTTCTAAGACAGACGCCTTATA15230.17225876138824953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15070.17044908300203024No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14560.16468073314595622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14490.16388899885198527No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAGT14090.15936480288643703No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13850.1566502853071081No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13550.1532571383329469No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13290.15031641095534054No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAGTGACAT13290.15031641095534054No Hit
TCTATAAGGCGTCTGTCTTAGAAACTGAGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13260.1499770962579244No Hit
GTCAGAGTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAG13250.1498639913587857No Hit
GTCTGCATCTATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGA13100.1481674178717051No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12640.14296459251132462No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12560.14205975331821497No Hit
GGCCAATAGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTATTGGAGACAGAGTCATCATCA12440.1407024945285505No Hit
CCTTATAGATTAGGAGCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGGCCAACCAGCCACTAAGACTCT12330.13945834063802473No Hit
GTAATACACGGCCGTGTCCTCGGCTTTAAGGCTGTTCATTTGCAGATACATTGAGTTCCGTGAATCATCTCTTGA12150.13742245245352802No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGATGGGGTAACC12140.13730934755438934No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11750.13289825648797976No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG11630.13154099769831532No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11470.129731319312096No Hit
GAGTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGT11430.12927889971554118No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11410.12905268991726376No Hit
ATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGAGTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAA11360.12848716542157024No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11010.12452849395171552No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10860.12283192046463492No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10740.12147466167497045No Hit
GTCCATGTAGTGGTCACTGAAGCTGAATCCAGACGCTACACAGGAGAGTCTCAGGGACCCTCCAGGCTGGACCAA10530.11909945879305762No Hit
GTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGT10480.11853393429736409No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAGTGACATCCAGATGACCCAGTCTCCTTCCACC10440.11808151470080927No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10320.11672425591114478No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10230.11570631181889643No Hit
ACTTATTACTGCCAGCACTATAACGGTTACCCCATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA10140.11468836772664807No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10130.11457526282750938No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10090.11412284323095456No Hit
CTCCTGTGTAGCGTCTGGATTCAGCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGG10050.11367042363439972No Hit
GTCTCCTTCCACCCTGTCTGCATCTATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGAGTCTTAGTG9920.11220005994559655No Hit
GCTTTAAGGCTGTTCATTTGCAGATACATTGAGTTCCGTGAATCATCTCTTGAGACGGTAAATCTGCCTTTCACA9800.11084280115593206No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9780.11061659135765466No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAGTGACATCCAGAT9700.109711752164545No Hit
GGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGTCTGTCTTAGAAACTGAGGTCCCGT9650.10914622766885149No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9640.10903312276971278No Hit
GTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGGCGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC9640.10903312276971278No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT9620.10880691297143535No Hit
GATTAGGAGCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGGCCAACCAGCCACTAAGACTCTGACTGGCCCG9340.10563997579555158No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9330.10552687089641288No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9270.10484824150158063No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC9250.10462203170330323No Hit
CCTCAGAATACGCCGCGTCTGTGAAAGGCAGATTTACCGTCTCAAGAGAT9200.1040565072076097No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9150.10349098271191616No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8890.10055025533430981No Hit
GTGATGGGGTAACCGTTATAGTGCTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGGCGATGGTGAGA8850.10009783573775499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA508.098977E-490.2651470
AATGGTA3050.073.9878270
GGATGCA700.003078464464.475170
ACTTCCA3950.053.3211870
ACCCTAT8950.047.06562470
AAAGTCA1951.7751481E-646.28981870
TATTCTA1357.354818E-444.5753870
GAGCAAA18900.044.5753870
GTGGTAT1750.042.674311
AAAGGCA6250.040.920270
AGACACA6800.039.82285770
GACAGGT1450.039.798141
GTATCAA15550.039.0754971
GCTTCGT350.001459367738.7948271
ATCTGCA7550.037.85955470
CGTCACA9550.036.2320870
AAGCGGT4702.1827873E-1135.2098170
TAGGCAA3008.455172E-735.1031170
ATAATAT400.00282708933.905163
ACATTTT4455.456968E-1233.8071770
Produced by FastQC (version 0.11.5)