FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967735
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAGTATAATGCCTCTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTG101951.0534908833513306No Hit
GTATAATGCCTCTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA52710.5446739034963084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38230.39504616449751223No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT37270.3851260934036694No Hit
CAGTAATACAGTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36630.37851271267444087No Hit
GCATTATACTGTTGACAGTAATACAGTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC34610.3576392297478132No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGACAACTGGTTCTTGGACGTGTCTACTGA32290.33366572460435967No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24770.2559585010359241No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22970.23735836773496874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20960.2165882188822353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20860.21555487814329338No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20630.21317819444372685No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20400.21080151074416034No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20050.20718481815786347No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19540.20191478038925947No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19410.2005714374286349No Hit
CTCCTGCAGGGCCAGTCAGAATATTGGCAGCAGCTACTTAGCCTGGTACC18910.1954047337339251No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18530.19147803892594564No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18440.1905480322608979No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC17410.17990462264979565No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16890.17453125080729745No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAGCAGCTACTTAGCC15760.16285450045725328No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15420.1593411419448506No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGGCTGAAGATTTTGCACTGTATTACTGTCAACAGTA15310.15820446713201444No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAG14610.1509710819594207No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14570.15055774566384392No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14530.15014440936826715No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14280.14756105752091223No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14270.14745772344701805No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCC14130.1460110464124993No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13900.14363436271293278No Hit
GTGTATTACTGTGCGAGAGGCTCGCGGGGTGCCAGCGGCTGGGGGCACTGGTTCGACCCCTGGGGCCAGGGAACC13790.14249768790009662No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCCAATATTCTGA13710.14167101530894305No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13580.1403276723483185No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGACAACTGGTTCTTGGACGTGTCTA13450.13898432938769395No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13340.1378476545748578No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13310.1375376523531752No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT13120.13557430494918546No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12340.12751424718543816No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12290.12699757681596718No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12080.12482756126418906No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12070.12472422719029487No Hit
CTGTATTACTGTCAACAGTATAATGCCTCTCCGTTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA11920.12317421608188192No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA11770.12162420497346899No Hit
GTAATACAGTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11660.12048753016063282No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11470.11852418275664309No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11460.11842084868274888No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGGCTGAAGATTTTGCACTGTATTACTGTCAACAGTATAATGCCTC11350.11728417386991273No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11290.11666416942654756No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG11010.11377081535751006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10990.11356414720972167No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10890.1125308064707797No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10790.11149746573183773No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10700.11056745906678998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10660.11015412277121318No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10500.10850077758890606No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10460.10808744129332927No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10110.1044707487070324No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC10080.10416074648534981No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10020.10354074204198464No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9960.10292073759861946No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9920.10250740130304267No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9830.1015773946379949No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9770.10095739019462974No Hit
GTCCCTCACCTGCGCTGTTTCTGGTTACTCCATCAGCAGTGATTACTACT9760.10085405612073553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTA200.007123800552.17620551
TGTTAGA8950.051.40302770
TATTCTA1601.061926E-745.74431670
CCGCGTA307.014173E-445.02414319
AAAACCG1050.001280628339.8317870
TATACAC3250.039.4663853
CGTATGC2900.039.06805841
GAGCAAA15100.038.77663870
CTTATAC3400.038.7259981
TCGTATG2950.038.34743540
TCTACTG8900.038.226868
GTATCAA21450.038.0894741
TAGGCAA1656.87968E-638.02124870
ATGCCGT3000.037.9152644
TACACAT3050.037.627575
CTACTGA11700.037.33144469
CGTCGGA2552.6739144E-936.90297770
GGCGGCG11150.036.57200670
ATCTCGT2750.036.006537
GCTAATC2650.035.9097133
Produced by FastQC (version 0.11.5)