FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179328
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT46060.3905614044608455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40850.34638370326151846No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38160.32357410321810387No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCTCGAACCACTCAGGCTGCTGTCATAG36450.3090743202908775No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACAGTCACCATCTC36430.3089047321864655No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTGGTCGTCTATGGTAACAACAATCGGCCCTCA35150.29805109350409725No Hit
GTAATAAACGGCCGTGTCCGCAGTGCTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCCACTGA29170.24734425028490803No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG29160.24725945623270204No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACAGTCAC28960.24556357518858196No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAG28650.2429349595701959No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27960.2370841699679818No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT27490.23309884951429968No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGAGGATATTCGGCGGAGGGACCAAGCTGACCGTC26970.22868955879958758No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26360.22351712161502144No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26050.22088850599663537No Hit
GTGCTAGACTCCCCCTCTCTCGCACAGTAATAAACGGCCGTGTCCGCAGTGCTCACAGAGCTCAACTTCAGGGA25140.21317224724588918No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25020.21215471861941718No Hit
CTCCTGTTCTGGGAGCAGCTCCAACATCGGGGCAGGTTCTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC24740.2097804851576491No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCTCGAACCACTC24700.2094413089488251No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGAGGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAA24540.20808460411352903No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA24320.206219134964997No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT24150.20477763607749497No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA23920.20282737287675695No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG23720.2011314918326369No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22450.19036264720247464No Hit
GTTCTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTGGTCGTCTATGGTAACAACAATCGGC22310.1891755304715906No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG21890.1856141802789385No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21210.17984818472893038No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21170.17950900852010637No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAT20710.17560848211863028No Hit
GCCCCCAAACTGGTCGTCTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGT20060.17009686872524016No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG19760.16755304715906008No Hit
GGCCATCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC19760.16755304715906008No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT19100.16195663971346394No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT19100.16195663971346394No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACAGTCAC18940.1605999348781679No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGTGTC18800.15941281814728386No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG18370.1557666739024258No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18120.15364682259727575No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCAGAACCTGCCCCGATGTTGGAGCTGCTCCCA18100.15347723449286374No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG17610.14932232593476963No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGCTC17410.14762644489064958No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17330.14694809247300156No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG16790.14236921365387747No Hit
GTGTCTGGGGCCCCAGGGCAGACAGTCACCATCTCCTGTTCTGGGAGCAGCTCCAACATCGGGGCAGGTTCTGAT16680.14143647907961143No Hit
GTGTACATCAGAACCTGCCCCGATGTTGGAGCTGCTCCCAGAACAGGAGA16270.13795992293916534No Hit
GTCTAGCACTAGTCGATACTACTACTACTACTATATGGACGTCTGGGGCA16190.13728157052151735No Hit
TCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGAG15770.13372022032886524No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15550.1318547511803332No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15380.13041325229283118No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGG15300.12973489987518316No Hit
GTTTATTACTGTGCGAGAGAGGGGGAGTCTAGCACTAGTCGATACTACTA15200.12888695935312314No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT15000.12719107830900309No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14700.12464725674282302No Hit
TCCAGGAACAGCCCCCAAACTGGTCGTCTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGAGCGATTCTCTG14510.12303616975090899No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14500.12295137569870299No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14400.12210343517664297No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG14390.12201864112443697No Hit
GGGCAGACAGTCACCATCTCCTGTTCTGGGAGCAGCTCCAACATCGGGGCAGGTTCTGATGTACACTGGTACCA14300.12125549465458296No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC14200.12040755413252292No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14200.12040755413252292No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGACGATG14180.12023796602811093No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA13830.11727017420090084No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT13780.11684620393987083No Hit
CTGTGAGCACTGCGGACACGGCCGTTTATTACTGTGCGAGAGAGGGGGAG13770.11676140988766484No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13670.11591346936560482No Hit
GTTCTGGGAGCAGCTCCAACATCGGGGCAGGTTCTGATGTACACTGGTAC13650.11574388126119281No Hit
GTGCTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCCACTGAGATGTTGACTCGACTCTTGAGG13420.11379361806045475No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13330.11303047159060076No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG12950.10980829760677266No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12800.10853638682368263No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12680.1075188581972106No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCTCGAACCA12650.10726447604059261No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12630.1070948879361806No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12600.10684050577956261No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12490.10590777120529657No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12450.10556859499647256No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCTCGAACCACTCA12390.10505983068323656No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAG12380.10497503663103055No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12330.10455106637000054No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12270.10404230205676451No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC12260.10395750800455852No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACAGTCACCATCTC12240.10378791990014653No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12040.10209203885602647No Hit
GCTTGGTCCCTCCGCCGAATATCCTCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCAT11980.10158327454279047No Hit
GATGTGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCGAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA11970.10149848049058446No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCA11840.10039615781190644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTT200.0063805454150.1900270
CACCTTT901.0017992E-466.7511270
AATGGTA3650.065.8367270
TGGATTT800.005260656656.3212670
GGTAGAA29000.054.8970470
TCTGAGT1451.6918459E-551.7896670
TCGTTAC200.00779205551.0070314
ACTAGCT200.007795974651.000548
TGCGTCA8550.050.94164370
CGGCATA900.00838190750.0633470
GGCAGTA19400.048.7730570
GTGGTAT2500.047.6348421
GGTATCA9100.043.7462841
TAAACGC405.451717E-542.500454
ATCTGGT2851.2067176E-842.158670
GTATCAA23000.038.019111
TGGTATC2800.037.6432532
AAAGTCA3203.351488E-837.54750470
AAACTCA2407.4936615E-637.547570
GGTGCCA20800.037.547570
Produced by FastQC (version 0.11.5)