FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407561
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46600.3310691330606631No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAACGTGTTCTTGGCGTTGTCTCTGGA39900.2834690645734004No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39730.282261301641634No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39630.28155085285824205No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34120.2424051248933439No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31420.2232230077417604No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC31100.22094957163490606No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCT29290.20809044865551118No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG28270.20084387106491297No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT28130.19984924276816424No Hit
GTGTATTACTGTGCAAGGGAAACAGTGGCTGATAAGGGCAAAAGATTTGA28110.19970715301148584No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG26340.18713220954544776No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25190.17896204853644No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23770.16887367581227386No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA22520.1599930660198741No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22470.1596378416281781No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT22060.15672500161627098No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTTTGACAGCAGCCTGAGTGGTTTTTATGTCTTCG21910.155659328441183No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAACCACTCAGGCTGCTGTCAAAG21740.15445156550941663No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGT21410.1521070845242231No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21060.14962051378235117No Hit
GAGTAGGATAGTCTATGCGGACCCCGTGAAGGGCCGATTCACCATCTCCA20980.1490521547556376No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT20540.1459261801087129No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAACCACTCA20180.14336856448850174No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC19470.13832437812641868No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGCCTGAGGTGCCAGACCGGGAGCCAGAGAATCGGTCAGGGACCCCTGAG19180.13626407665458193No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18910.1343458649394236No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCACTGGGCTCCAGACTGAGGATG18890.13420377518274518No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18670.13264078785928282No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAACCA18650.13249869810260442No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACCA18100.12859122979394852No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17570.12482585124197103No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG17250.12255241513511671No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAACCACTC17210.12226823562175991No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG17170.12198405610840313No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT16970.12056315854161917No Hit
GGATGAGGCTGATTATTATTGCCAGTCCTTTGACAGCAGCCTGAGTGGTTTTTATGTCTTCGGAACTGGGACCAA16900.12006584439324477No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16750.1190001712181568No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT16700.1186449468264608No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG16570.11772136340805124No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC16480.11708195950299845No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16370.11630046584126727No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCA16270.11559001705787528No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16220.11523479266617931No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT16150.11473747851780491No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC15640.11111418972250581No Hit
GTGATGGCCAGGGAGCCTGAGGTGCCAGACCGGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT15600.11083001020914902No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGAT15190.10791717019724188No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14850.10550164433370916No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG14760.10486224042865638No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGGCTGCGTCAGCACAGACTGGGCCCAG14620.1038676121319076No Hit
TCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTTT14530.10322820822685481No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG14290.10152313114671406No Hit
CCTCAGGCTCCCTGGCCATCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTTTGACAGCA14140.1004574579716261No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT14120.1003153682149477No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14090.1001022335799301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTA200.0075849416141.7556570
GTTGTAT13400.045.48875470
GAGCCAT18350.044.80559570
GGTATCA12150.041.627211
GTGGTAT3900.040.9072721
GCAATAA16050.040.62778570
GTACAGT3506.730261E-1140.50161470
GTATCAA30400.038.0757141
ACTGATT2257.1604845E-637.80150270
GTAGTTT1901.1738404E-437.30411570
ACCCAGT1550.001945622836.582170
AAGCGGT12000.035.4389170
AATGGTA3654.5492925E-934.95344570
GTATCGA400.002818070633.9286521
CTTAACG400.002823476233.9153862
CAGCCGT14800.033.52329370
AAAAGGT16150.033.35426770
ACTCAGT12400.033.1525370
TTAACGG1450.032.93781335
TGGTATC4750.032.8443762
Produced by FastQC (version 0.11.5)