FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482176
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGGTGATGGTGAGAGTAAATTCTGTC142460.9611544108122112No Hit
GTACAATAGTTACCCGTACACTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCA74660.5037188566000259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54150.36534122803229846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT51050.34442603307569414No Hit
GAGTAAATTCTGTCCCAAATCCGGTGCCGCTGAACCTTGATGAGACCGCATCTTGTAAACCTGACGCCCTATAAA49310.3326865365516646No Hit
GTGTTAGTCAGTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCCCG42220.28485146163478564No Hit
GTCCTGGATATCAGGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA41340.2789142450019431No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38710.26117006347424326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT35740.24113195733839976No Hit
GTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA35490.23944524806770584No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34430.23229360075996375No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33880.22858284036443716No Hit
ATCCAGGACGGTCCCCTGGCCAAAAGTGTACGGGTAACTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTC32140.2168433438404076No Hit
GTCAGAGTGTTAGTCAGTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGGG31630.21340245692819207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31070.20962422816183773No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30180.20361954315816747No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29630.1999087827626409No Hit
GAGTGTTAGTCAGTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCC29200.19700764281704738No Hit
GAATTTACTCTCACCATCACCGACTTGCTGCCGGACGATTTTGGAACATATTTCTGCCAACAGTACAATAGTTAC29070.1961305539962865No Hit
AGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28850.1946462498380759No Hit
ACCTTGATGAGACCGCATCTTGTAAACCTGACGCCCTATAAATCAGAAGTTTCGGGACTTTGCCTGGTTTCTGG28280.19080055270089383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC28220.19039574247592728No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28160.18999093225096075No Hit
CATCAAGGTTCAGCGGCACCGGATTTGGGACAGAATTTACTCTCACCATCACCGACTTGCTGCCGGACGATTT28090.18951865365516646No Hit
ATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGG27780.18742713415950601No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27140.18310915842652964No Hit
GGTTTACAAGATGCGGTCTCATCAAGGTTCAGCGGCACCGGATTTGGGACAGAATTTACTCTCACCATCACCGA26510.17885865106438104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25910.17481054881471567No Hit
GACTAACACTCTGACTGGCCCGGCAAGTGATCGTGATTGTGTCTCCCACAAAAGCAGACAGCGAAGAAGGAGA25680.1732587762856773No Hit
CTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCA25340.17096485167753356No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24310.16401560948227473No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCATATGACCCAGTCTCCTTCTTCG24220.16340839414482491No Hit
GATTTATAGGGCGTCAGGTTTACAAGATGCGGTCTCATCAAGGTTCAGCGGCACCGGATTTGGGACAGAATTTA23750.16023738071592036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23290.1571338356578436No Hit
GTCTCCTTCTTCGCTGTCTGCTTTTGTGGGAGACACAATCACGATCACTT22990.15510978453301094No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22520.1519387711041064No Hit
TTGTAAACCTGACGCCCTATAAATCAGAAGTTTCGGGACTTTGCCTGGTT22120.14924003627099616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21850.14741839025864673No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21390.14431484520056997No Hit
CCATATGACCCAGTCTCCTTCTTCGCTGTCTGCTTTTGTGGGAGACACAA21320.14384256660477568No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACA21000.1416835787382875No Hit
GTGATGGTGAGAGTAAATTCTGTCCCAAATCCGGTGCCGCTGAACCTTGA20860.1407390215466989No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20460.13804028671358867No Hit
ATAGTTACCCGTACACTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCATCTGTCTTCA19790.13351990586812904No Hit
CACTCTGACTGGCCCGGCAAGTGATCGTGATTGTGTCTCCCACAAAAGCAGACAGCGAAGAAGGAGACTGGGTCA19400.13088863940584652No Hit
GGCCAAAAGTGTACGGGTAACTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGGTGA18860.12724534738114773No Hit
CTATAAATCAGAAGTTTCGGGACTTTGCCTGGTTTCTGGTGATACCAGGCCAACCACTGACTAACACTCTGAC18620.12562610648128159No Hit
GTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGGGCGTCAGGTTTACAAGA18610.12555863811045384No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18470.12461408091886525No Hit
GGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGGGCGTCAGGTTTACAAGATGCGGTCTCA18430.12434420743555422No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18330.12366952372727665No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17960.12117319400664968No Hit
GGTAACTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGGTGATGGTGAGAGTAAAT17620.11887926939850599No Hit
ATATGACCCAGTCTCCTTCTTCGCTGTCTGCTTTTGTGGGAGACACAATC17610.11881180102767822No Hit
CAGTACAATAGTTACCCGTACACTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCA17490.11800218057774516No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17310.11678774990284554No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17020.11483116714884063No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16920.11415648344056306No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCATA16900.11402154669890754No Hit
CTAACACTCTGACTGGCCCGGCAAGTGATCGTGATTGTGTCTCCCACAAA16680.11253724254069691No Hit
GTGCAGCCACAGTTCGCCTGATATCCAGGACGGTCCCCTGGCCAAAAGTG16290.10990597607841443No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC15880.10713977287447644No Hit
GCTCCACACCATGGACTGGACCTGGACGATCCTCTTCTTGGTGGCAGCAG15500.10457597478302173No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15500.10457597478302173No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14900.10052787253335636No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14840.10012306230838984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA14350.050.71639370
CTAGTAC200.00821486850.3303383
TCGTATG2100.044.0179840
GTGGTAT4500.042.512651
CGTATGC2350.039.41662641
GCGTAGA30800.038.69714470
GAGCAAA23500.038.59950670
TTGTACG350.001545145838.348224
GTTATTA1150.00191921836.6870970
GTATCAA37850.036.089791
ACGAGAC2900.035.88333522
TGAGAGT35850.034.6829259
AGAGTAA36000.034.5509361
GAGAGTA36500.034.06284360
ATTCTGT39350.033.8197568
AAAACCG2508.5830834E-833.75212570
CGGTGAT36750.033.52592550
GTATGCC3100.033.26579742
TTCTGTC50900.032.78691569
ATCTCGT2700.032.76853637
Produced by FastQC (version 0.11.5)