FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482176
Sequences flagged as poor quality0
Sequence length18-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGGTGATGGTGAGAGTAAATTCTGTC134460.9071797141500064No Hit
GTACAATAGTTACCCGTACACTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCAT69580.4694449242195258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA51180.3453031218964549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49050.33093235891014294No Hit
GAGTAAATTCTGTCCCAAATCCGGTGCCGCTGAACCTTGATGAGACCGCA47520.32060969817349627No Hit
GTGTTAGTCAGTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGGGCGTCAG40250.2715601925817177No Hit
GTCCTGGATATCAGGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT39900.26919879960274623No Hit
GAATTTACTCTCACCATCACCGACTTGCTGCCGGACGATTTTGGAACATATTTCTGCCAACAGTACAATAGTTAC38050.2567171509996114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37670.25415335290815666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT35980.2427511982382659No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34750.23445258862645194No Hit
GTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA33840.22831296688112612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC33500.22601904227298242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA30900.20847726585776588No Hit
ATCCAGGACGGTCCCCTGGCCAAAAGTGTACGGGTAACTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTC30120.20321473293320094No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29870.20152802366250702No Hit
GAGTGTTAGTCAGTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCC29660.20011118787512414No Hit
GGTTTACAAGATGCGGTCTCATCAAGGTTCAGCGGCACCGGATTTGGGACAGAATTTACTCTCACCATCACC29590.19963890927932987No Hit
ACCTTGATGAGACCGCATCTTGTAAACCTGACGCCCTATAAATCAGAAGTTTCGGGACTTTGCCTGGTTTCTGGT28110.18965359039682197No Hit
AGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27990.1888439699468889No Hit
GTCAGAGTGTTAGTCAGTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGG27250.18385131050563497No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26800.18081523381838593No Hit
GACTAACACTCTGACTGGCCCGGCAAGTGATCGTGATTGTGTCTCCCACAAAAGCAGACAGCGAAGAAGGA26620.17960080314348634No Hit
ATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGG26550.17912852454769204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26260.17717194179368712No Hit
CATCAAGGTTCAGCGGCACCGGATTTGGGACAGAATTTACTCTCACCATCACCGACTTGCTGCCGGACGATTTTG25580.1725840925773997No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24820.16745649639449026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC24540.1655673820113131No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA24510.1653649768988298No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24180.16313852066151388No Hit
GTCTCCTTCTTCGCTGTCTGCTTTTGTGGGAGACACAATCACGATCACTTGCCGGGCCAGTCAGAGTGTTAGTCA24040.1621939634699253No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCATATGACCCAGTCTCCTTCTTC23740.16016991234509262No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22670.15295079666652273No Hit
GATTTATAGGGCGTCAGGTTTACAAGATGCGGTCTCATCAAGGTTCAGCGGCACCGGATTTGGGACAGAATTTA22110.14917256790016842No Hit
CTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT21930.1479581372252688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21860.1474858586294745No Hit
CCATATGACCCAGTCTCCTTCTTCGCTGTCTGCTTTTGTGGGAGACACAATCACGATCACTTGCCGGGCCAGTCA20860.1407390215466989No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA20740.13992940109676583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20630.13918724901766052No Hit
TTGTAAACCTGACGCCCTATAAATCAGAAGTTTCGGGACTTTGCCTGGTTTCTGGTGATACCAGGCCAACCACT20040.13520661513882293No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCATAT20010.13500421002633967No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19860.1339921844639233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT19590.1321705384515739No Hit
GTGGTTGGCCTGGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGGGCGTCAGGTTTACAA19020.1283248413143918No Hit
GTGATGGTGAGAGTAAATTCTGTCCCAAATCCGGTGCCGCTGAACCTTGATGAGACCGCATCTTGTAAACCTGAC18770.1266381320436979No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18720.12630079018955914No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACAT18710.12623332181873137No Hit
ATAGTTACCCGTACACTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGA18120.12225268793989379No Hit
GGTATCACCAGAAACCAGGCAAAGTCCCGAAACTTCTGATTTATAGGGCGTCAGGTTTACAAGATGCGGTCTCAT17500.1180696489485729No Hit
CACCATCACCGACTTGCTGCCGGACGATTTTGGAACATATTTCTGCCAAC17350.11705762338615658No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17250.11638293967787901No Hit
CACTCTGACTGGCCCGGCAAGTGATCGTGATTGTGTCTCCCACAAAAGCA17050.1150335722613239No Hit
GGTAACTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGGTGATGGTGAGAGTAAATT16880.11388660995725204No Hit
GGCCAAAAGTGTACGGGTAACTATTGTACTGTTGGCAGAAATATGTTCCAAAATCGTCCGGCAGCAAGTCGGT16660.11240230579904141No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16330.11017584956172546No Hit
ATATGACCCAGTCTCCTTCTTCGCTGTCTGCTTTTGTGGGAGACACAATCACGATCACTTGCCGGGCCAGTCAGA16310.11004091282006995No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACATCCATAT16110.10869154540351483No Hit
CTATAAATCAGAAGTTTCGGGACTTTGCCTGGTTTCTGGTGATACCAGGCCAACCACTGACTAACACTCTGACTG16110.10869154540351483No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15890.1072072412453042No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15400.10390129107474416No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG15300.10322660736646659No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC15120.102012176691567No Hit
GTGCAGCCACAGTTCGCCTGATATCCAGGACGGTCCCCTGGCCAAAAGTG15090.10180977157908373No Hit
CAGTACAATAGTTACCCGTACACTTTTGGCCAGGGGACCGTCCTGGATATCAGGCGAACTGTGGCTGCACCAT15040.10147242972494495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)