FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022733
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT52940.5176326568126774No Hit
CCTTAGGAGGCTGTCCCGGTTTCTGCTGATACCATGCTAAGTAGTTCTTATTGTTGAAACTATATAGGAGAC52180.5102015873155554No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49790.4868328292917115No Hit
CTCCTAAGGTGATCATCTACTGGGCTTCTACCCGAGAAACCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTC46580.4554463383893939No Hit
ATATTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCA38260.3740956828419539No Hit
CTCCTATATAGTTTCAACAATAAGAACTACTTAGCATGGTATCAGCAGAA37080.3625579696753698No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35860.3506291475878846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT34700.33928698888175113No Hit
TTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCT31280.30584717614470247No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30880.30193608693569096No Hit
CTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACGT30840.3015449780147898No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC30790.30105609186366333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29080.284336185495139No Hit
CCTATATAGTTTCAACAATAAGAACTACTTAGCATGGTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGATCA28950.2830650815022102No Hit
GTACTGTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAA28710.28071842797680335No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28400.2776873338398194No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26370.2578385561040858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26150.2556874570391295No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25890.25314524905327196No Hit
CCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGC25130.24571417955615002No Hit
CTGTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAATCTGTC25100.2454208478654742No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25030.24473640725389714No Hit
ATATAGGAGACTCTGGCTGGACCTGCAATTAATGGTGGCCCTCTCGCCCAGAGACACACCCAGGGAGTCTGGAGA23450.22928760487830155No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23280.22762539196447165No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAG22870.22361652552523484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC22280.21784766894194282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT22240.2174565600210417No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCGTGGTCCCTTGGCCGAACGTG21750.2126654757400025No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21190.20718995084738637No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21140.20670106469625993No Hit
ACTTAGCATGGTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGATCATC20900.204354411170853No Hit
GTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGCCAGAGTCTCCTATA20060.19614112383192878No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19820.19379447030652186No Hit
GATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTAC19000.18577673742804818No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCGTGGTCCCTTGGCCGAACGTGCGAGGAGTA18630.1821589799097125No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17150.1676879498363698No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17120.16739461814569395No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGA16940.16563462800163875No Hit
GTGTTGGTGGACGTGTCTTTGGTCATGGTGACTCTGCCCTGATACTTCTG16600.16231020217397893No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16570.16201687048330307No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16560.1619190932530778No Hit
CTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC16470.16103909818105017No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTA16450.1608435437205996No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16220.15859466742541797No Hit
ACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCA16190.15830133573474212No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16080.15722578620226393No Hit
CTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGCCAGAGTCTCCTATATAGTTTCAA15590.1524347019212248No Hit
GAGTACTGTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAA15550.15204359300032363No Hit
GTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGCCAGAGTCTCCTATATAGTTTCAACAATAAGAACTA15220.14881694440288912No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14770.14441696904275114No Hit
CCATTAATTGCAGGTCCAGCCAGAGTCTCCTATATAGTTTCAACAATAAG14610.14285253335914652No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14450.1412880976755419No Hit
GTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGATCATCTACTGGGCTTCTACCCGAGAAACCGGGGTCCCTGA14210.13894144415013499No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13920.1361059044736016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13460.13160815188323835No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13430.13131482019256246No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13240.12945705281828201No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12910.12623040422084747No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12850.12564374083949573No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAAT12760.12476374576746814No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGG12730.12447041407679228No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12530.12251486947228651No Hit
CTCTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACG12500.12222153778161066No Hit
GAACTACTTAGCATGGTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGA12480.12202598332116006No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12470.12192820609093478No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12460.1218304288607095No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12420.12143931993980833No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG12360.1208526565584566No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12100.1183104485725991No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12080.11811489411214854No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG12040.11772378519124738No Hit
GTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAA12040.11772378519124738No Hit
GCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTC11990.11723489904012094No Hit
CTATATAGGAGACTCTGGCTGGACCTGCAATTAATGGTGGCCCTCTCGCC11980.11713712180989565No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11940.11674601288899449No Hit
GTCTGGAACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCCTCTGGTTACACGTTCAGCAG11940.11674601288899449No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11880.11615934950764276No Hit
CACCAACACAGTCCACATGGAGCTGAGGAGCCTGAAATCTGACGACACGG11840.1157682405867416No Hit
GTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGC11800.11537713166584045No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11800.11537713166584045No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11750.114888245514714No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGG11700.11439935936358755No Hit
CTACTGGGCTTCTACCCGAGAAACCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTC11650.11391047321246113No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11630.11371491875201054No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11550.11293270091020824No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11380.11127048799637831No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11350.11097715630570247No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT11280.11029271569412545No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11270.11019493846390015No Hit
GTCTGGGACAGATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATA11220.10970605231277371No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG11070.10823939385939439No Hit
GTCAGGGACCCCGGTTTCTCGGGTAGAAGCCCAGTAGATGATCACCTTAGGAGGCTGTCCCGGTTTCTGCTGATA11030.10784828493849323No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10930.10687051263624035No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10870.10628384925488862No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10650.10413275018993227No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10480.10247053727610238No Hit
GTCTATGCACAGAAGTATCAGGGCAGAGTCACCATGACCAAAGACACGTC10420.10188387389475062No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10410.10178609666452534No Hit
TCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10400.10168831943430005No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCG10360.10129721051339889No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10290.10061276990182189No Hit
GTGTGTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGCCAGAGT10290.10061276990182189No Hit
GCCACAGTTCGCTTGATTTCCACCGTGGTCCCTTGGCCGAACGTGCGAGGAGTACTGTAATATTGCTGACAATAA10280.1005149926715966No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10240.10012388375069545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAT150.00253165267.75609622
TTCGAAG550.005508254355.67374470
CGTATGC750.054.90862741
CGCGTAT252.66809E-454.77020639
TTGTAAA8100.049.14410870
TCGCGTA352.3927441E-548.85849838
TCGTATG652.2737368E-1047.46063240
TGTTAGA11500.044.37762570
TATACAC2050.042.948373
ATCTCCG2000.042.3247810
TCTCCGA2000.042.3247811
CGAGACT2100.041.950423
TACACAT2100.041.9278375
ATACACA2100.041.9257854
CCCACGA2100.040.31325519
TTATACA2200.040.0181162
GTGGTAT1950.039.9471
CTTATAC2150.039.3815161
GCGTATG350.001391367439.17385540
GCGTAGA28300.038.5912870
Produced by FastQC (version 0.11.5)