FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022733
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49810.4870283837521621No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA48130.47060180907431365No Hit
CCTTAGGAGGCTGTCCCGGTTTCTGCTGATACCATGCTAAGTAGTTCTTATTGTTGAAACTATATAGGAGACT45240.44234418953920523No Hit
CTCCTAAGGTGATCATCTACTGGGCTTCTACCCGAGAAACCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG42820.4186820998246854No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35950.3515091426599122No Hit
CTCCTATATAGTTTCAACAATAAGAACTACTTAGCATGGTATCAGCAGAA33570.32823816186629357No Hit
ATATTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCAT33350.3260870628013372No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT32450.3172871120810612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC32000.3128871367209233No Hit
CTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACGTT31790.3108338148861922No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29090.2844339627253643No Hit
GTACTGTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAAT28660.2802295418256769No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26290.2570563382622835No Hit
CTGTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAATCTGTC26040.2546119075066513No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25830.25255858567192024No Hit
TTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTT25480.24913638261403515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC24630.24082531804488563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24000.23466535254069243No Hit
CCTATATAGTTTCAACAATAAGAACTACTTAGCATGGTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGATCAT24000.23466535254069243No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23670.23143870394325794No Hit
CTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGTTTCTCGGGTAGAA23640.23114537225258205No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22400.21902099570464628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22030.2154032381863106No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21950.21462102034450828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21760.2127632529702278No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21680.21198103512842548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21590.2111010400563979No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21170.2069943963869358No Hit
CCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGC20910.20445218840107832No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCGTGGTCCCTTGGCCGAACGTG20810.2034744160988254No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGTTTCTCGGGTAGAAGCCCAGTA20040.1959455693714782No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA19980.19535890599012645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT19250.1882211681836804No Hit
ATATAGGAGACTCTGGCTGGACCTGCAATTAATGGTGGCCCTCTCGCCCAGAGACACACCCAGGGAGTCTGGA19040.18616784634894934No Hit
GATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTACT17830.17433680149168942No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGTTTCTCGGGTAGAAGCCCAGTAGATGATCACCTTA17810.17414124703123884No Hit
GTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATT17790.17394569257078826No Hit
GTGTTGGTGGACGTGTCTTTGGTCATGGTGACTCTGCCCTGATACTTCTG17550.17159903904538135No Hit
ACTTAGCATGGTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGATCATCTACTGGGCTTCTACCCGAGAAACCG17500.1711101528942549No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17230.16847016767817213No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCGTGGTCCCTTGGCCGAACGTGCGAGGAGTA17200.16817683598749625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16630.1626035338646548No Hit
GAGTACTGTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAAT16220.15859466742541797No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15770.15419469206528No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCGGCC15730.15380358314437884No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15520.15175026130964778No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15470.15126137515852134No Hit
CTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGCCAGAGTCTCCTATATAGTTTCAA14950.14617695918680634No Hit
GTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGATCATCTACTGGGCTTCTACCCGAGAAACCGGGGTCCCT14680.14353697397072354No Hit
ACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGA14170.13855033522923382No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13940.1363014589340522No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13920.1361059044736016No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGAT13920.1361059044736016No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13810.13503035494112345No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG13720.13415035986909585No Hit
TCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC13530.13229259249481537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13440.13141259742278777No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13220.1292614983578314No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGG13110.12818594882535325No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13020.12730595375332565No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGT12990.1270126220626498No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12930.12642595868129805No Hit
GTCTCTGGGCGAGAGGGCCACCATTAATTGCAGGTCCAGCCAGAGTCTCCTATATAGTTTCAACAATAAGAACTA12900.1261326269906222No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12700.12417708238611641No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12650.12368819623498996No Hit
CCATTAATTGCAGGTCCAGCCAGAGTCTCCTATATAGTTTCAACAATAAG12470.12192820609093478No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12300.12026599317710487No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12140.11870155749350027No Hit
CTCTCCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCA12080.11811489411214854No Hit
GTCTGGAACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCCTCTGGTTACACGTTCAGCA11990.11723489904012094No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT11740.11479046828448872No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11660.11400825044268642No Hit
GATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGTTTCTCGGGTAGAAGC11640.11381269598223583No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11620.11361714152178526No Hit
GTCTGGGACAGATTTCACTCTCTCCATCAGCGGCCTGCAGGCTGAAGATG11610.11351936429155997No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11570.1131282553706588No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT11450.11195492860795536No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11420.11166159691727948No Hit
GTAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGC11410.1115638196870542No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11380.11127048799637831No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGG11360.11107493353592775No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG11250.10999938400344958No Hit
CACCAACACAGTCCACATGGAGCTGAGGAGCCTGAAATCTGACGACACGG11210.10960827508254842No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATTAATT11170.10921716616164728No Hit
GAACTACTTAGCATGGTATCAGCAGAAACCGGGACAGCCTCCTAAGGTGA11080.10833717108961968No Hit
GCCAAGGGACCACGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTC11060.10814161662916909No Hit
GTTTATTATTGTCAGCAATATTACAGTACTCCTCGCACGTTCGGCCAAGGGACCACGGTGGAAATCAAGCGAA10990.10745717601759208No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT10980.10735939878736679No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGG10740.10501274526195986No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTG10670.10432830465038284No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA10640.104034972959707No Hit
GTCAGGGACCCCGGTTTCTCGGGTAGAAGCCCAGTAGATGATCACCTTAGGAGGCTGTCCCGGTTTCTGCTGATA10590.10354608680858055No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG10510.10276386896677824No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10440.10207942835520122No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10300.10071054713204716No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10260.10031943821114601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)