FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023720
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA42820.41827843550970967No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41660.40694721212831636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT41180.4022584300394639No Hit
TTATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGGGTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTC39200.3829172039229477No Hit
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGACTTCAGACTCAGGGAGA36390.35546829211112413No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG35040.34228109248622673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29310.2863087563005509No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT27310.26677216426366585No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25580.24987301215176028No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24940.24362130269995702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23940.23385300668151446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22500.2197866604149572No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22300.21783300121126872No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAAGACATTAAGACTTCTTTAAATTGGTACCAGCAGAAA22160.21646543976868676No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22030.2151955612862892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21840.21333958504278516No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC21250.20757629039190403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21090.20601336302895323No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19850.19390067596608446No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAATTGGATCCGCCATGACCCAGAGGAGGGCCTGGAG19330.18882116203649435No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18820.18383933106708866No Hit
CATTAAGACTTCTTTAAATTGGTACCAGCAGAAACCAGGGAAAGCCCCTAATCTCCTGATCTTCGATGCATCCAA18680.1824717696245067No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18070.17651310905325673No Hit
GTCTATTACTGTGCGAGAGCCTATCGATTCTTGTCCTTTGACTACTGGGGCCTGGGAACCCCGGTCACCGTCTC16850.16459578791075685No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16740.16352127534872818No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG16500.16117688430430194No Hit
GTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTCCCAGACCCACTTCCACTGAACCTTGATGGGACTCCTGTCTC16390.1601023717422733No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACA16210.15834407845895362No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC16110.15736724885710937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15570.1520923690071504No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15260.1490641972414332No Hit
CCCTGAAGATATTGCAACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAG15070.1472082209979291No Hit
ACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14950.146036025475716No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCTACTCGCAGATGGGTC14860.1451568788340562No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14340.14007736490446607No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14150.13822138866096198No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAAGACATTAAGACTTCTTTAAATTGGTA13870.1354862657757981No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13870.1354862657757981No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAAGACATTAAGA13800.1348024850545071No Hit
GTGATATGTTGCAATATCTTCAGGGTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTCCCAGACCCACTTCCA13770.1345094361739538No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13530.1321650451295276No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA13360.13050443480639237No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13260.12952760520454812No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13250.1294299222443637No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13140.12835540968233503No Hit
GGACATTATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGGGTGT12990.12689016527956862No Hit
GTCTTAATGTCTTGACTGGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGACGGAGA12790.12493650607588014No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC12770.12474114015551127No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12340.12054077286758097No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12160.11878247958426133No Hit
GTGGAAGTGGGTCTGGGACATTTTTCACTTTCACCATCTCCAGCCTACACCCTGAAGATATTGCAACATATCA12110.11829406478333919No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12090.11809869886297035No Hit
GATTAGGGGCTTTCCCTGGTTTCTGCTGGTACCAATTTAAAGAAGTCTTAATGTCTTGACTGGCCTGGCAAGTGA12080.11800101590278593No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12070.1179033329426015No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12000.11721955222131053No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATG11760.1148751611768843No Hit
CAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11720.11448442933614661No Hit
ATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA11710.11438674637596219No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11570.11301918493338023No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11470.11204235533153596No Hit
ATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGGGTGTAGGCTGG11400.11135857461024498No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11390.11126089165006056No Hit
GCCTACACCCTGAAGATATTGCAACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGA11380.11116320868987614No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11170.1091118665260032No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11070.10813503692415896No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10830.10579064587973273No Hit
CAGTAATAGACGGCCGTGTCCGCGGCAGTCACAGACTTCAGACTCAGGGA10810.1055952799593639No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10790.10539991403899504No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10750.10500918219825733No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTC10600.10354393779549095No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10470.10227405931309341No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10440.10198101043254015No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10420.10178564451217131No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10410.10168796155198687No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG10250.10012503418903607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA150.009380491132.0299470
CACGGAA9250.087.7820770
AATCGAG400.0017584374.26684670
AACCGGA702.2693265E-456.58425570
AATGGTA3600.052.26185670
TGGTATC2200.049.3476522
GTGGTAT2450.047.093191
GGTATCA7800.044.8113371
GTATCAA19550.039.0553931
TGTTAGA11150.037.29993470
GATTCAC1550.037.218811
GAGCAAA19550.035.9621270
TCTCGCG8050.035.67268870
TCGTATG2050.035.12978740
TATCTCG2000.034.1668436
ATTCACT1700.033.926512
TCTCCGA2200.033.92319511
GACAGGT2050.033.1071171
CGTATGC2200.032.76369541
ATCAACG23350.032.547793
Produced by FastQC (version 0.11.5)