FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023720
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40860.3991325753135623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA39630.387117571210878No Hit
GTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT39130.3822334232016567No Hit
TTATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGGGTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTC35340.3452115812917595No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG32830.3206931582854687No Hit
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGACTTCAGACTCAGGGAGA32620.31864181612159576No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28970.2829875356542805No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT26730.26110655257296916No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC25430.24840776774899387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23110.22574532098620717No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22380.2186144648927441No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22190.21675848864924No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21600.21099519399835892No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAAGACATTAAGACTTCTTTAAATTGGTACCAGCAGAAA21030.20542726526784666No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20960.2047434845465557No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA20140.19673348181143283No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20000.19536592036885086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19820.1936076270855312No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19110.186672136912437No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAATTGGATCCG18760.18325323330598212No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18300.17875981713749856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17750.17338725432735513No Hit
CATTAAGACTTCTTTAAATTGGTACCAGCAGAAACCAGGGAAAGCCCCTA17230.16830774039776503No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17110.1671355448755519No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16390.1601023717422733No Hit
GTCTATTACTGTGCGAGAGCCTATCGATTCTTGTCCTTTGACTACTGGGGCCTGGGAACCCCGGTCACCGTCTC16330.15951627398116672No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCA16330.15951627398116672No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16320.1594185910209823No Hit
GTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTCCCAGACCCACTTCCACTGAACCTTGATGGGACTCCTGTCTC16090.15717188293674053No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCTACTCGCAGATGGGTC15580.15219005196733482No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG15550.15189700308678153No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15320.14965029500253976No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15170.14818505059977338No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14820.1447661469933185No Hit
CCCTGAAGATATTGCAACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAA14380.14046809674520377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14270.13939358418317507No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAAGACATTAAGACTTCTTTAAATTGGTA14010.13685382721838002No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAAGACATTAA13960.1363654124174579No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGT13440.13128589848786779No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13250.1294299222443637No Hit
GTCTTAATGTCTTGACTGGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGACGGA12910.12610870159809323No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCA12830.12532723791661782No Hit
ACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12780.12483882311569569No Hit
GTGATATGTTGCAATATCTTCAGGGTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTCCCAGACCCACTTCCA12640.12347126167311376No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC12440.12151760246942524No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12420.12132223654905637No Hit
GCCTACACCCTGAAGATATTGCAACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGG12350.12063845582776539No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12300.12015004102684328No Hit
GTGGAAGTGGGTCTGGGACATTTTTCACTTTCACCATCTCCAGCCTACACCCTGAAGATATTGCAACATATCA12140.11858711366389248No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11920.1164380885398351No Hit
GGGTGTAGGCTGGAGATGGTGAAAGTGAAAAATGTCCCAGACCCACTTCCACTGAACCTTGATGGGACTC11900.11624272261946625No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11870.11594967373891299No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.11467979525651545No Hit
GGACATTATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGGGTGT11710.11438674637596219No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11590.11321455085374907No Hit
ATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA11450.11184698941116712No Hit
AGCCTACACCCTGAAGATATTGCAACATATCACTGTCAACAGTATGATAATGTCCCTTACAATTTTGGCCAGGG11290.11028406204821632No Hit
GATTAGGGGCTTTCCCTGGTTTCTGCTGGTACCAATTTAAAGAAGTCTTAATGTCTTGACTGGCCTGGCAAGT11260.10999101316766302No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11210.10950259836674092No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA11200.10940491540655647No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11120.10862345172508107No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG11070.10813503692415896No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10960.10706052436213027No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10880.10627906068065487No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10820.10569296291954831No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10720.10471613331770406No Hit
CAGTATGATAATGTCCCTTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10650.10403235259641308No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10620.10373930371585979No Hit
ATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGGGTGTAGGCTGG10540.10295784003438439No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG10540.10295784003438439No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10370.10129722971124917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAA8950.0130.3545470
ATGGCAA2300.065.64400570
AATGGTA4050.057.61348370
TTGGACG200.00691914852.56244359
TTGATTT1402.3414901E-549.01987570
GGTATCA7750.045.864681
TAGTCGG306.779704E-445.3361830
GTGGTAT2300.044.1554341
GTATCAA22450.041.4674261
TGGTATC2500.039.2324522
CGCCGTA350.001466525739.215970
AGTCCGC350.001473245838.7207621
TTCACGG9600.037.38177568
TATTCTA1500.001939641336.6015170
TTACCAA1500.001939641336.6015170
TATCAAC25800.036.1806872
ATCAACG26050.035.9562723
AAGCGGT7350.035.48105270
TCAACGC26600.034.9602784
AACGCAG27050.034.5053836
Produced by FastQC (version 0.11.5)