FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777552
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCACCCTCATCTTCAGACCGGAGCCCACTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA35640.4583616272609421No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACCAATCAGGCTGTCATCCCATGCTGCACAGTAAT28550.367178015103813No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGATTGGTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTA28330.364348622342943No Hit
GTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC28320.36422001358108524No Hit
GTCCTGGGCCCAGTCTGTGATGACTCAGCCACCCTCAGCGTCTGGGACCC27620.3552174002510443No Hit
ATATTACACCAATCAGGCTGTCATCCCATGCTGCACAGTAATAATCACCC26870.34557174311171474No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA25670.33013869168878734No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23490.3021019816038027No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG22910.2946426734160545No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCCTCAGCGTCT21810.28049570961170445No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21710.27920962199312716No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC20950.2694353560919398No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACCAATCAGGCTGTCATCCCATG20470.2632621355227689No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19990.257088914953598No Hit
CTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTC18640.23972673210280468No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC18620.23946951457908924No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT17180.22094985287157642No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17020.21889211268185277No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGATGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16630.21387637096940143No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16470.21181863077967775No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15970.20538819268679137No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15940.2050023664012182No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15750.20255879992592138No Hit
GGCCATTAGTGGGCTCCGGTCTGAAGATGAGGGTGATTATTACTGTGCAGCATGGGATGACAGCCTGATTGGTGT15430.19844331954647407No Hit
GTATTAGTTCCAATATTGGGGCTGCTTCCAGAACAAGAGATGGTGACCCT15380.19780027573718545No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15320.19702862316603903No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC15190.19535670926188858No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15140.19471366545259994No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15110.19432783916702678No Hit
GTCTGTGATGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC15060.19368479535773814No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14750.18969792374014857No Hit
CCCCTGAGGGCCGCTGATCATTACCATAAATGAGGACCTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTT14740.18956931497829085No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14450.18583966088441672No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14420.18545383459884357No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCAGTGCTCGTGGATGTGTCTGTGGT13850.17812313517295306No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13550.17426487231722124No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13460.17310739346050166No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13430.17272156717492848No Hit
GCATGGGATGACAGCCTGATTGGTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG12870.16551947651089574No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTC12610.1621756487025948No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12480.16050373479844435No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGG12460.16024651727472888No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12270.15780295079943205No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12160.15638825441899706No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC12040.15484494927670434No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC11930.1534302528962693No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG11870.15265860032512296No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGT11610.14931477251682201No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11560.14867172870753337No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA10990.1413410292816429No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10770.1385116365207729No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATTACACCAATC10750.13825441899705743No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10740.1381258102351997No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCCCCAATATTGGAACTAAT10670.13722554890219563No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10480.1347819824268988No Hit
CATTTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTC10310.13259563347531741No Hit
GAACTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCCCAAG10270.1320811984278865No Hit
CTCTTGTTCTGGAAGCAGCCCCAATATTGGAACTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCC9940.12783710928658149No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9910.1274512830010083No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAG9810.126165195382431No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9800.1260365866205733No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC9560.12294997633598781No Hit
GATCATTACCATAAATGAGGACCTTGGGGGCCGTTCCTGGGAGATGCTGG9550.12282136757413009No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC9490.12204971500298371No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGTCTGAAGATG9380.12063501862254872No Hit
GCCCCAATATTGGAACTAATACTGTAAACTGGTACCAGCATCTCCCAGGA9350.12024919233697554No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9340.1201205835751178No Hit
ATGTATTACTGTGCGAGAGATCGTGATTACGTTTGGGGGACTTATCGTCACGTTTCTGAATACTGGGGCCAGGGA9290.11947753976582916No Hit
ATCCCATGCTGCACAGTAATAATCACCCTCATCTTCAGACCGGAGCCCACTAATGGCCAGGGAGGCTGAGGTGCC9280.11934893100397144No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT9220.11857727843282506No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9210.11844866967096734No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9140.11754840833796325No Hit
GCTTGGTCCCTCCGCCGAATATTACACCAATCAGGCTGTCATCCCATGCTGCACAGTAATAATCACCCTCATCTT9100.11703397329053233No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9060.11651953824310143No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGG8980.11549066814823959No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTC8890.11433318929152006No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA8880.11420458052966231No Hit
GTATTACTGTGCGAGAGATCGTGATTACGTTTGGGGGACTTATCGTCACGTTTCTGAATACTGGGGCCAGGGAAC8790.11304710167294278No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8730.1122754491017964No Hit
GTGATGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCCCC8660.11137518776879231No Hit
GTGCAGCATGGGATGACAGCCTGATTGGTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCA8590.11047492643578821No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTG8530.10970327386464185No Hit
ATGCTGCACAGTAATAATCACCCTCATCTTCAGACCGGAGCCCACTAATG8520.10957466510278413No Hit
CAGTAATACATGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTA8450.10867440376978003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8450.10867440376978003No Hit
CCCAGGAACGGCCCCCAAGGTCCTCATTTATGGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGG8350.10738831615120276No Hit
CCTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTACAGTATTAGTTCCAATATTGGGGCTGCTTCCAGAAC8350.10738831615120276No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGATTGGTGTAATATTCGGCG8250.10610222853262546No Hit
TTACTGTGCAGCATGGGATGACAGCCTGATTGGTGTAATATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTC8210.10558779348519456No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG8100.10417309710475954No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC8100.10417309710475954No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAG8000.10288700948618226No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC7960.10237257443875138No Hit
CAGTAATAATCACCCTCATCTTCAGACCGGAGCCCACTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC7960.10237257443875138No Hit
GTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACCCCTGAGGGCCGCTG7950.10224396567689363No Hit
ATATTGGAACTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCCCAAGGTCCTCATTTATGGTAATG7910.10172953062946273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1650.080.6337870
TTGGGTA150.002314042569.3077550
CGCGTAT253.2419412E-668.4573139
TTGCGAG150.002522786867.814524
CGTATGG309.450334E-657.1590941
TCGCGTA309.6117565E-656.99595638
ATTTAAT700.007302973451.83600270
GAAGAAT700.007302973451.83600270
GCGTTCC200.00737742751.71566846
TATACAC1450.051.44553
GCGTATG552.763045E-949.8291940
ATCGCGT352.4083312E-548.8030337
ATACACA1700.047.8690724
TACACAT1750.046.504385
GGTATCA6150.046.3242721
CGTATGC451.9383588E-645.72727241
TCTCGCG5300.045.64176670
CGAGACT1650.045.20968223
CTTATAC1700.043.8912731
ACGAGAC1700.043.87998222
Produced by FastQC (version 0.11.5)