FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132714
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAATGCCAGCGCCCCAGGTCTGACAGTAATAGTCAG38630.3410393091283413No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36720.32417715327964514No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG33740.2978686588141402No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG32650.2882457531203817No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAATGCCAGCGCCCCAGGTCTGACAG31490.27800486265729923No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC30850.27235471619490886No Hit
GGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC29910.2640560635782731No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC29730.26246695988572577No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTT28010.24728219126805176No Hit
GATTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCT27160.23977809049768964No Hit
CTGTTAATCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTACCTGCTG25910.22874264818833354No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGGTCGTGGATGTGTCTGTGG25720.2270652609573114No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25540.22547615726476408No Hit
GACTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG25110.2216799651103456No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTC23720.20940855326234162No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23100.20393497387690096No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22170.19572460479874002No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22150.1955480377217903No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21290.18795565341295334No Hit
CAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG21140.1866314003358306No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21050.18583684848955695No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21040.18574856495108208No Hit
CACCTGCACTCTGAGCAGTGGCCACAGCAGGTACGCCATCGCATGGCATC20980.185218863720233No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20890.18442431187395936No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20720.18292349171988692No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20460.18062811971954085No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC20300.17921558310394328No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC19680.17374200371850265No Hit
CTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCC19610.1731240189491787No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC19610.1731240189491787No Hit
CCTGCACTCTGAGCAGTGGCCACAGCAGGTACGCCATCGCATGGCATCAG19560.17268260125680446No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19240.1698575280256093No Hit
GCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG19160.1691512597178105No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA18940.1672090218713638No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18830.16623790294814048No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC18650.1646487992555932No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGG18080.1596166375625268No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17880.15785096679302985No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC17550.15493761002335982No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT17270.15246567094606406No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG17060.15061171663809222No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT16890.14911089648401982No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16750.1478749269453719No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16720.14761007632994738No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCAC16670.1471686586375731No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG16110.1422247804829816No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAAG16030.14151851217518277No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAA15910.1404591097134846No Hit
AGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCG15700.1386051554055128No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15440.13630978340516672No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15230.1344558290971949No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15000.13242530771227337No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAATGCCAG14740.1301299357119273No Hit
GTACTTGATGAAGATTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG14280.12606889294208423No Hit
GTCTATTACTGTGCGAGAGATTGGAATTGTAGTGGTGGTCGCTGCCTCGA14180.12518605755733575No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14100.12447978924953694No Hit
CCCTTGTTGTGGCTGCCATCACTGTTAATCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCA14080.12430322217258725No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACT13780.12165471601834178No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGC13550.11962419463342026No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13540.11953591109494541No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG13430.11856479217172207No Hit
CTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCAC13300.11741710617154905No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13260.11706397201764963No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT13230.1167991214022251No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13160.11618113663290117No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC13050.11521001770967781No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCC12950.11432718232492933No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC12830.11326777986323114No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12720.11229666094000781No Hit
GCTTGGTCCCTCCGCCGAATACCCGAATGCCAGCGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA12690.11203181032458326No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGCCACAGC12570.11097240786288506No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCT12550.11079584078593538No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12490.1102661395550863No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12360.10911845355491324No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12360.10911845355491324No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG12350.1090301700164384No Hit
CAGCAGGTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGATTAACAGTGA12280.10841218524711446No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12240.10805905109321505No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12210.10779420047779052No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12120.10699964863151686No Hit
GGCCACAGCAGGTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGG12070.10655823093914263No Hit
GAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTTGTGGCTGCCATCACTGTTAATCTTCATCAAG11970.10567539555439415No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11880.10488084370812051No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11860.10470427663117081No Hit
CCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGA11850.10461599309269595No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11760.10382144124642231No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11720.10346830709252293No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11690.10320345647709837No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11680.10311517293862352No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT11650.10285032232319898No Hit
GCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCC11500.10152606924607624No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11410.1007315173998026No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAAGCTGACCG11400.10064323386132774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2200.079.5054970
TATTCTA1403.7107384E-1062.46859470
GTGGTAT1900.048.2045971
TCGTATG1800.045.6604940
CAGGGAA8500.045.0141470
CGTATGC1850.044.48988341
GTATGCC1950.042.2479642
TATCTCG1700.042.1602636
GACGGAC1950.041.76644526
ATTCAGA1050.001028895541.64573770
ATGCCGT2000.041.280344
CTTATAC2000.040.7061041
TATAAGA1250.040.691732
GACCGGA1357.2457595E-540.48890770
CGAGACG2050.039.70800823
TTATACA2100.038.754032
CACGAGA2150.037.8561121
TCTCCGA2150.037.85276811
GTATAAG1350.037.6908341
ATAAGAG1350.037.6775253
Produced by FastQC (version 0.11.5)