FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132714
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC37760.33335864128102943No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAATGCCAGCGCCCCAGGTCTGACAGTAATAGTCA35770.31579021712453453No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34390.30360708881500537No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT33180.29292478065954863No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32670.2884223201973313No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCG29050.2564636792694361No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC27550.24322114849820872No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGGTCGTGGATGTGTCTGTGGT26980.23818898680514236No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGA25280.22318078526441804No Hit
CTGTTAATCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTACCTGCTGTG23780.20993825449319067No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23750.20967340387776615No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGTT23720.20940855326234162No Hit
GACTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCA23640.2087022849545428No Hit
GATTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGA23360.20623034587724703No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23300.20570064464639795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22260.19651915664501365No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA21860.1929878151060197No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT21370.1886619217207521No Hit
GCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAA21270.1877790863360036No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20790.18354147648921088No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20780.183453192950736No Hit
CAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCA20290.1791272995654684No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20140.1778030464883457No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC20120.177626479411396No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG20040.1769202111035972No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG19800.17480140618020085No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19630.1733005860261284No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG19540.17250603417985477No Hit
AGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCA19450.17171148233358113No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA18900.16685588771746443No Hit
CCTGCACTCTGAGCAGTGGCCACAGCAGGTACGCCATCGCATGGCATCAG18720.16526678402491715No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18410.16252999433219684No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG18380.16226514371677228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG18220.1608526071011747No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT18220.1608526071011747No Hit
CACCTGCACTCTGAGCAGTGGCCACAGCAGGTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCG18170.16041118940880048No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17920.15820410094692922No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC17700.15626186310048257No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGG17690.1561735795620077No Hit
CTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCC17260.15237738740758922No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC17100.15096485079199162No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCGCTGGCATTCGGGTATTCGGCGGAGGGACCAA16890.14911089648401982No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16880.14902261294554495No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG16830.1485811952531707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT16820.14849291171469586No Hit
GTCTATTACTGTGCGAGAGATTGGAATTGTAGTGGTGGTCGCTGCCTCGA16720.14761007632994738No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG15420.136133216328217No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC15200.13419097848177033No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACT15160.13383784432787094No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT15120.13348471017397154No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAATGCCA14910.13163075586599973No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT14800.13065963694277638No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14770.13039478632735185No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14710.12986508509650274No Hit
CTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT14520.1281876978654806No Hit
GTACTTGATGAAGATTAACAGTGATGGCAGCCACAACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCA14500.1280111307885309No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC14330.12651031063445847No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT14280.12606889294208423No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGC13820.12200785017224118No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG13580.11988904524884482No Hit
GTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGC13550.11962419463342026No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13510.11927106047952088No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC13320.11759367324849875No Hit
CCCTTGTTGTGGCTGCCATCACTGTTAATCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCAT13100.11565143540205206No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12780.1128263621708569No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12780.1128263621708569No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTACTGACTCAATCGCCCTCTGCCTCTGCCT12760.1126497950939072No Hit
GCTTGGTCCCTCCGCCGAATACCCGAATGCCAGCGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA12490.1102661395550863No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCGCTGGCATTCG12410.10955987124728749No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCA12370.1092067370933881No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA12330.10885360293948869No Hit
CAGCAGGTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGATTAACAGT12320.10876531940101385No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCT12120.10699964863151686No Hit
CCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCA11950.10549882847744445No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11940.10541054493896959No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG11900.1050574107850702No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT11770.10390972478489716No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG11720.10346830709252293No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTT11680.10311517293862352No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11560.10205577047692532No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGCGATTGAGTCAGTACAAGCTGGGAGA11470.10126121863065168No Hit
GGCCACAGCAGGTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGG11420.10081980093827744No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCT11390.1005549503228529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT1351.0913936E-1193.28187670
AATGGTA3000.089.200870
AACTCGC150.002328254369.2029250
TACTAAG150.002549660467.6355442
CAGGGAA10100.046.75639370
GGTAGAA29050.044.43332370
TGACAGT44000.044.0041470
CAGCCAT12300.043.51258570
TATTCTA1150.00739577541.0643170
GTGGTAT3000.040.6153951
TTACTTT1600.001358427839.35329470
ACCTCCA9700.037.3247770
AAACTCA2151.3104288E-436.6077170
GTATCAA20750.036.2113151
TTCAGGT1750.002109553235.98015670
ATCTTCA22100.035.61384270
TATTGTT4105.0767994E-934.5541170
CCCTACA11150.033.8826670
ATCAACG22750.032.6985663
AGTGGAA15050.032.42397770
Produced by FastQC (version 0.11.5)