FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162723
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACACTCCCCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA43250.37197165618982336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40550.348750304242713No Hit
GTGTAAGCCTGTTGACAGTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGGC40090.3447940739109831No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT38210.32862513255521736No Hit
GTTTACTACTGTCAACAGGCTTACACTCCCCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA29950.257584996598502No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29190.25104861605042644No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGATGTCAACTTCAGGGAGAATTGCTTCTTGGACTTGTCCACTGA29110.2503605759927343No Hit
GTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGGCCCAGATCCACTGCCA26730.22989138427639255No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGATGTCAACTTCAGGGAGAATTGCTTCTTGGACTTGTCCA26270.22593515394466265No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG26040.2239570387787977No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25450.21888274335331803No Hit
CTATAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCGTTAGC25340.21793668827399132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24560.21122829771149276No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24300.20899216752399324No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23670.2035738520696675No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23450.20168174191101404No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23070.1984135516369763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22590.19428531129082335No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22140.19041508596630496No Hit
GTATTATTGTGCGAGGGGGGGATTTTTGGGACATTCTGGGGCCCAGGGAAGGCTGGTCACCGTGTCCTCAGCATC22040.18955503589418976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20740.17837438495669217No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20680.17785835491342306No Hit
GTGTAGGGGGGAGTGTAAGCCTGTTGACAGTAGTAAACTGCGAAATCTCC20530.17656827980525025No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20160.17338609453842405No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19920.17132197436534755No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19740.16977388423554018No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19480.16753775404804067No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT19120.16444157378842594No Hit
GTGTATTATTGTGCGAGGGGGGGATTTTTGGGACATTCTGGGGCCCAGGGAAGGCTGGTCACCGTGTCCTCAGCA18270.1571311481754468No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17870.15369094788698598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC17820.15326092285092838No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG17480.1503367526057367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17110.14715456733891047No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16910.14543446719468006No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16910.14543446719468006No Hit
GTCATGGACCTCCTGCACAAGAATATGAAACACCTGTGGCTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16710.14371436705044968No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCGTTAGCCCCTATTTAAATTGGTATCAACAAGAA16460.14156424187016167No Hit
CCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG16160.1389840916538161No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGGGGGAG16080.13829605159612393No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA15550.13373778621391338No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15440.13279173113458664No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15100.12986756088939497No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14840.12763143070189548No Hit
ACCTTGAGGGGACCCCACTTTGCAAATTGGATGTTCTAGAAATCAGGAGCTTAGGGGCTTTCCCTGGTTCTTGT14750.12685738563699178No Hit
GTCTCCATCCTCCCTGTCTGCCTCTATAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCGTTAGCCC14710.12651336560814572No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG14610.1256533155360305No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14590.12548130552160747No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14540.12505128048554986No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14150.1216970852043006No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14140.12161108019708908No Hit
CTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGGTTGGGGAAATCAATGAGAGTGGAAACAC14100.12126706016824298No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14030.12066502511776235No Hit
GGCCAAAAGTGTAGGGGGGAGTGTAAGCCTGTTGACAGTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCTGA13840.11903092998074348No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13610.11705281481487852No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA13560.11662278977882092No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13320.11455866960574444No Hit
GTCCCTCACCTGCGCTGTGAATGGTGGCTCCTTCAGTGCTTACTACTGGA13290.11430065458410989No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13190.1134406045119947No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13130.11292457446872557No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13120.11283856946151405No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG13080.11249454943266797No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13030.11206452439661038No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12930.11120447432449516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12910.11103246431007213No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12780.10991439921632237No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12750.10965638419468782No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12720.10939836917305326No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12700.10922635915863022No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12700.10922635915863022No Hit
GTTCAGTGGCAGTGGATCTGGGCCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGGAGATTTCGCAGTTTA12670.10896834413699566No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12530.10776427403603438No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12450.10707623397834222No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12320.10595816888459245No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA12300.10578615887016941No Hit
GTGAATGGTGGCTCCTTCAGTGCTTACTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGGT12180.10475409878363119No Hit
CCTCAAGGTTCAGTGGCAGTGGATCTGGGCCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGGAGATTTCG12070.10380804370430446No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11910.10243196358892014No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11710.10071186344468976No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11690.1005398534302667No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11680.10045384842305519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)