FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162723
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72910.6270625075791912No Hit
GTGTAAGCCTGTTGACAGTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGGC39520.33989178849992646No Hit
GGCTTACACTCCCCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38510.33120528277156297No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37800.3250989272595451No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA37210.3200246318340654No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGATGTCAACTTCAGGGAGAATTGCTTCTTGGACTTGTCCACT28060.2413300502355247No Hit
GTTTACTACTGTCAACAGGCTTACACTCCCCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA27950.240383995156198No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27070.23281555452158426No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGATGTCAACTTCAGGGAGAATTGCTTCTTGGACTTGTCCA26750.2300633942908156No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT26470.22765525408889306No Hit
GTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGGCCCAGATCCACTGCCA26270.22593515394466265No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25120.2160445781153379No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24080.20710005736533982No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA23940.20589598726437852No Hit
CTATAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCGTTAGCCCCTATTTAAATTGGTATCAACAA23060.19832754662976476No Hit
GTATTATTGTGCGAGGGGGGGATTTTTGGGACATTCTGGGGCCCAGGGAAGGCTGGTCACCGTGTCCTCAGCATC21740.18697488567784418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21640.18611483560572897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20690.17794435992063456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20210.17381611957448162No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA20180.17355810455284706No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20140.17321408452400097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19800.17028991427880932No Hit
GTGTAGGGGGGAGTGTAAGCCTGTTGACAGTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCTGATGGTGAGA18360.15790519324035046No Hit
GTGTATTATTGTGCGAGGGGGGGATTTTTGGGACATTCTGGGGCCCAGGG18080.1554970530384279No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17550.15093878765621735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA16460.14156424187016167No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16350.14061818679083496No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT16140.13881208163939304No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16120.13864007162497No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16010.13769401654564328No Hit
GTCATGGACCTCCTGCACAAGAATATGAAACACCTGTGGCTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15990.13752200653122024No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15660.1346838412932401No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15600.13416781124997096No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15340.13193168106247147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15260.1312436410047793No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15200.13072761096151017No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT15060.1295235408605489No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14640.12591133055766507No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCGTTAGCCCCTATTTAAATTGGTATCAACAAGAA14570.12530929550718442No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14540.12505128048554986No Hit
CCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC14500.12470726045670379No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCCTCTATA14430.12410522540622314No Hit
GTCTCCATCCTCCCTGTCTGCCTCTATAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCGTTAGCCC14280.12281515029805036No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGGGGGAGT14230.12238512526199276No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13810.11877291495910892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13780.11851489993747436No Hit
GTCCCTCACCTGCGCTGTGAATGGTGGCTCCTTCAGTGCTTACTACTGGACCTGGATCCGCCAGCCCCCAGGGAA13760.11834288992305132No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13730.11808487490141675No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13730.11808487490141675No Hit
ACCTTGAGGGGACCCCACTTTGCAAATTGGATGTTCTAGAAATCAGGAGCTTAGGGGCTTTCCCTGGTTCTTGTT13500.11610675973555182No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12530.10776427403603438No Hit
CTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGGTTGGGGAAATCAATGAGAGTGGAAACAC12480.10733424899997679No Hit
GGCCAAAAGTGTAGGGGGGAGTGTAAGCCTGTTGACAGTAGTAAACTGCGAAATCTCCAGGTTGCAGACTGCT12440.1069902289711307No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12400.1066462089422846No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12330.10604417389180398No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12290.1057001538629579No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12180.10475409878363119No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12170.10466809377641967No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12060.10372203869709294No Hit
GTTCAGTGGCAGTGGATCTGGGCCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGGAGATTTCGCAGTTTA12050.10363603368988142No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT11850.10191593354565101No Hit
CCTCAAGGTTCAGTGGCAGTGGATCTGGGCCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGGAGATTTC11790.10139990350238191No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11780.1013138984951704No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11690.1005398534302667No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11660.10028183840863215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA753.3031334E-583.5000270
TCAGCAT13000.061.42069270
GTGGTAT2250.046.3877031
CACTCCA9800.044.7321670
AATGGTA3306.4028427E-1042.6988870
CCAGAGT8200.042.00458570
GAGCAAA22100.040.38038370
GAGCCAT15000.039.66251470
GACAGGT3250.037.2943341
AAGGACA23950.035.95382370
ACTCAGT11550.035.24351570
CTCGTAT1800.001149142734.79167670
AAGCGGT13500.034.79167670
GACCCAT5053.092282E-1134.10273770
TACCCTT400.002938584733.6395345
TCCTATA2500.032.295344
ATGACTT3401.6488939E-632.23346770
ATCAACG29800.031.8320183
TCAACGC30000.031.2861144
GTCTCCT2700.031.174531
Produced by FastQC (version 0.11.5)