FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397446
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA120020.8588525066442639No Hit
GTATTATATTGTTGACAATAATAAGATGCAGAATCATCAGGCTGCAGGCTGCTAATGTTTAGAGTGAATTCTGTC116270.8320178382563619No Hit
GAGTATTACTAAGTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATGTACAAGGCG73860.5285356285681164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64500.4615562962719132No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT52310.37432573423230664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49760.35607815972853335No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC39990.2861649036885862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38440.27507324075492007No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38330.27428609048220826No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38150.272998026399589No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37630.2692769523831332No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36480.26104765407750996No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35420.25346238781319635No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33440.23929368290438413No Hit
GTAATACTCTGACTGGCCCGGCAAGTAATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGAC33430.23922212378868307No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT32610.23335427630119518No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29930.21417643329330796No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29290.20959664988843935No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28630.20487374825216859No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA27670.1980040731448657No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTACTTGCCG27560.19721692287215392No Hit
TAGTAATACTCTGACTGGCCCGGCAAGTAATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA27350.19571418144243138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26880.19235090300448102No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCACCCACTTAGTAATACTCTGACTGGCCCGGCAAGTAA26770.19156375273176923No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26730.19127751626896494No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC26590.19027568864914995No Hit
GTATTACTAAGTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATGTACAAGGCGTCTA25890.18526655055007493No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATTACTTGCCGGGCCAGTCAGAGTATTACTAAGTGGGTGGCCTGGTA25610.1832628953104449No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC24870.1779675207485656No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG24770.17725192959155486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24650.17639322020314202No Hit
GCCAAGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC24420.17474736054201737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24150.17281526441808845No Hit
GTAGGAGACAGAGTCACCATTACTTGCCGGGCCAGTCAGAGTATTACTAAGTGGGTGGCCTGGTATCAGCAGAAA23240.1663033848892909No Hit
ATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCA23060.16501532080667158No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG23040.16487220257526944No Hit
CATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCACCC22040.1577162910051623No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGATCCAGTCTCCTTCCACC21780.1558557539969344No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21420.15327962583169583No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20780.1486998424268272No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20500.1466961871871972No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG19920.14254575847653506No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTGTACATCAGGACCTTAGGGGCTTTCCCTGGTTTC19790.14161548997242113No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTC19710.14104301704681255No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19510.1396118347327911No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19230.1376081794931611No Hit
GTCCTGATGTACAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAA19170.13717882479895468No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTAAACATTAGCAGC19080.13653479275764502No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18430.13188345023707534No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18120.12966511765034214No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18110.12959355853464105No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18060.12923576295613567No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17700.1266596347908971No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17610.12601560274958745No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17520.1253715707082778No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT17270.12358259281575101No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17260.12351103370004995No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG17190.12301011989014243No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC17000.12165049669182208No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT16720.11964684145219207No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG16460.1177863044439642No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16220.1160688856671385No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16100.11521017627872561No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16100.11521017627872561No Hit
TTCTAAAGTAGACGCCTTGTACATCAGGACCTTAGGGGCTTTCCCTGGTT16020.11463770335311706No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15950.11413678954320954No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15800.11306340280769346No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGATCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG15770.11284872546059024No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15750.1127056072291881No Hit
TGTTTAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCT15680.1122046934192806No Hit
CCATTACTTGCCGGGCCAGTCAGAGTATTACTAAGTGGGTGGCCTGGTAT15560.11134598403086775No Hit
CTGCTAATGTTTAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCT15500.11091662933666131No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15470.1107019519895581No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15300.10948544702263988No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGA15160.10848361940282486No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15160.10848361940282486No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15090.10798270559291737No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14880.10647996416319484No Hit
CAATAATAAGATGCAGAATCATCAGGCTGCAGGCTGCTAATGTTTAGAGTGAATTCTGTCCCAGATCCACTGCCG14800.10590749123758629No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14780.10576437300618413No Hit
ATCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT14700.10519190008057555No Hit
GGGTGGAAGGAGACTGGATCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG14380.10290200837814126No Hit
CCCCTAAGGTCCTGATGTACAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATC14240.10190018075832627No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC14240.10190018075832627No Hit
GGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14200.10161394429552197No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGC14150.10125614871701662No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTGTACA14070.10068367579140804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA500.003865325460.88812370
AATGGTA7150.056.77214470
TAGGTCG200.007435424351.61553645
TTTCGCG200.007674138551.20462838
GTGGTAT2450.045.4842531
TGTTAGA19600.041.4204970
GTATCAA25200.037.2526781
GCGTAGA37900.036.95057770
GACAGGT2850.034.3610461
TATCAAC28400.033.042182
GACAATA28750.032.9933414
GGAAACG1851.8509934E-532.912570
CGTATGC1800.032.3546941
TGACAAT29500.032.26664413
ATCAACG29900.031.272773
CGGCAAA1300.004229752331.2246870
TAAGATG30650.031.18061822
ATAAGAT30650.031.17615721
TCAACGC30050.031.117784
TATATTG31100.031.0440965
Produced by FastQC (version 0.11.5)