FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397446
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATATTGTTGACAATAATAAGATGCAGAATCATCAGGCTGCAGGCTGCTAATGTTTAGAGTGAATTCTGTC115170.8241463355292441No Hit
ATATAATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCAT111670.799100645033869No Hit
GAGTATTACTAAGTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATGTACAAGGCGT66510.4759396785278286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA61330.43887205659467343No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51060.36538084476967264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49260.35250020394347975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC39090.2797245832754897No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA38640.2765044230689415No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT36900.264053136936955No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35350.25296147400328883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34890.24966975468103955No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32560.2329964807226898No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA32460.2322808895656791No Hit
GTAATACTCTGACTGGCCCGGCAAGTAATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTG31430.2249103006484687No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC30780.22025895812789903No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA29010.20759299464880934No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28870.2065911670289943No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28610.20473063002076647No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28540.20422971621085895No Hit
TAGTAATACTCTGACTGGCCCGGCAAGTAATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGA28060.20079487865720752No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26770.19156375273176923No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA26150.1871270875583028No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25610.1832628953104449No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25420.18190327211212456No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTACTTGCCGGGCCAGTCAGAGTATTACTAAGTGG24930.17839687544277202No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT24670.17653633843454417No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT24390.17453268319491413No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATTACTTGCCGGGCCAGTCAGA23990.1716703185668713No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCACCCACTTAGTA23900.17102628652556162No Hit
GTATTACTAAGTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATGTACAAGGCGTCTA23600.16887951305452947No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23290.16666118046779624No Hit
ATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAAACGA22430.16050709651750406No Hit
GCCAAGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC22250.15921903243488478No Hit
GTAGGAGACAGAGTCACCATTACTTGCCGGGCCAGTCAGAGTATTACTAAGTGGGTGGCCTGGTATCAGCAGAAA22200.15886123685637943No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT22140.158431882162173No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21990.15735849542665692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21960.15714381807955372No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21260.15213467998047867No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20620.14755489657561008No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTC20430.14619527337728971No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGATCCAGTCTCCTTCCACC20100.14383382255915433No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT19990.14304667228644255No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19620.1403989850055029No Hit
GTCCTGATGTACAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTT19120.1368210292204493No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18980.1358192016006343No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTGTACATCAGGACCTTAGGGGCTTTCCCTGGTTTCT18980.1358192016006343No Hit
CATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCACCCACTTAGTAATACTCTGACTGGCCCG18880.1351036104436236No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA18780.13438801928661287No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18730.13403022370810752No Hit
CTGCTAATGTTTAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGTA18620.1332430734353957No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC18510.13245592316268393No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTAAACATTAGCAGC18110.12959355853464105No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17970.12859173091482606No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17840.1276614624107121No Hit
GGGTGGAAGGAGACTGGATCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGA17730.12687431213800032No Hit
TGTTTAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGG17580.12580092540248425No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17410.12458442043556603No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17170.1228670016587403No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA17080.12222296961743066No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17080.12222296961743066No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16890.12086334641911028No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT16670.11928904587368672No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16310.11671291770844812No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG15880.11363587573330204No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT15700.11234781165068275No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15480.11077351110525917No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15110.1081258238243195No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15080.10791114647721631No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14830.1061221685846895No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14810.10597905035328735No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14780.10576437300618413No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGATCATCTGGATGTCACATTTGGCACCTGGGAGCCAGA14690.10512034096487449No Hit
CAATAATAAGATGCAGAATCATCAGGCTGCAGGCTGCTAATGTTTAGAGTGAATTCTGTCCCAGATCCACTGCC14620.104619427154967No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTGTACATCAGGACCTTA14600.10447630892356484No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14590.10440474980786378No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT14400.1030451266095434No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14060.10061211667570698No Hit
GATAAGTATTATATTGTTGACAATAATAAGATGCAGAATCATCAGGCTGCAGGCTGCTAATGTTTAGAGTGAATT14030.10039743932860376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA500.00112568783.098170
TACGTAT756.0692502E-573.8649870
GCGCGTA200.005427207355.89156766
AATGGTA6200.046.91021770
GTGGTAT3000.046.0725361
TCGGTTT1309.1877487E-442.6144170
AATTTGT6450.040.7975270
GACAGGT2500.035.0600781
ATGGCAA3757.4305717E-933.23924370
CAGCCAT11300.033.09216370
GTATCAA23850.033.0755421
CGTCACA17950.032.40594570
GCAAATT2203.184418E-431.47655570
GAGCAAA41350.031.1492370
GACAATA28600.031.11395314
TATATTG29250.030.5180975
GGTACGT704.0885247E-430.17014968
TGACAAT29900.029.75051913
TAATAAG30200.029.49087519
GTATTAT30100.029.455661
Produced by FastQC (version 0.11.5)