FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087799
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT45390.4172645865642458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45220.415701797850522No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31790.29224148946634443No Hit
GTCCCAGACTCCTCATCTATGAAGCATCCAACAGGGCCCCTGGCACCCCAGCCAGGTTCAGCGGCGGTGGGTC31720.2915979882312817No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29300.26935123124768456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27210.25013812294366883No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26260.24140489189638897No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25680.23607302452015494No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25470.23414252081496675No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25360.2331313045884396No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25340.2329474470927074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25300.232579732101243No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCA23200.21327469504936114No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC22830.2098733313783153No Hit
GTCTGGGACCCTGGCCAGGTTTCAGTTGGTACCAGGCTAAGTCGTTGCGA21650.19902573913011504No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAA21320.19599209045053362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21240.19525666046760476No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21140.19433737298894374No Hit
GACTTCACTCTCACCATCAGCCGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCACCAGCGTAGCAAC20460.18808621813404866No Hit
GTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAACGACTTAGCCTGGTA20440.18790236063831645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20120.18496064070660112No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAACGACTTAGCCTGGTACCAACTGAAACCTGGCCAGGGTCCCAGAC19120.17576776591999074No Hit
GGCCAAAACTGTACCTCCGAGGCCAGTTGCTACGCTGGTGACAGTAATAA19090.1754919796763924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18670.17163097226601606No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18560.17061975603948892No Hit
TCTATGAAGCATCCAACAGGGCCCCTGGCACCCCAGCCAGGTTCAGCGGCGGTGGGTCTGGGACGGACTTCAC17230.1583932325732971No Hit
GTCTGGGACGGACTTCACTCTCACCATCAGCCGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCACCAGCG16850.15489994015438516No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCTCCGAGG16290.14975193027388334No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15890.14607478035923915No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15860.14579899411564085No Hit
GTATTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGCGAAAGAGC15620.14359270416685438No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA15450.14202991545313057No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15130.13908819552141527No Hit
GAGTGAAGTCCGTCCCAGACCCACCGCCGCTGAACCTGGCTGGGGTGCCAGGGGCCCTGTTGGATGCTTCATAGA14530.13357247064944902No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14520.13348054190158293No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14480.13311282691011853No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14280.13127425195279643No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT14160.13017110697840317No Hit
GTCAGAGTGTTCGCAACGACTTAGCCTGGTACCAACTGAAACCTGGCCAGGGTCCCAGACTCCTCATCTATGAAG14130.12989532073480486No Hit
GCGTAGCAACTGGCCTCGGAGGTACAGTTTTGGCCAGGGGACCAAGCTGG14020.12888410450827772No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCTCCGAGGCCAGTTGCTACG13960.1283325320210811No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13560.12465538210643694No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13310.12235716340978434No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13120.12061051720032837No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12760.11730108227714862No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12270.11279657363170953No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12210.1122450011445129No Hit
GACTTAGCCTGGTACCAACTGAAACCTGGCCAGGGTCCCAGACTCCTCATCTATGAAGCATCCAACAGGGCCC12100.11123378491798576No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12100.11123378491798576No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11960.1099467824478603No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11930.109670996204262No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTG11850.10893556622133317No Hit
GTCCCAGACCCACCGCCGCTGAACCTGGCTGGGGTGCCAGGGGCCCTGTTGGATGCTTCATAGATGAGGAGTC11730.10783242124693991No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11650.10709699126401111No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11530.10599384628961785No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11510.10580998879388565No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11510.10580998879388565No Hit
GTTCAGCGGCGGTGGGTCTGGGACGGACTTCACTCTCACCATCAGCCGCCTAGAGCCTGAAGATTTTGCAATTTA11400.10479877256735848No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11300.10387948508869746No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11170.10268441136643809No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11160.102592482618572No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10930.10047812141765161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)