FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087799
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43830.40292370189713356No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA41990.38600881228977046No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32170.29573478188525637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30030.27606202984191014No Hit
GTCCCAGACTCCTCATCTATGAAGCATCCAACAGGGCCCCTGGCACCCCAGCCAGGTTCAGCGGCGGTGGGTCTG29990.2756943148504457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT27920.2566650640421622No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA27140.2494946217086061No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC26010.23910667319973636No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25660.2358891670244227No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24510.22531736101982078No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23280.21401012503228997No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22760.20922983014325258No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21440.19709523542492685No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21410.19681944918132854No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT21410.19681944918132854No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20780.19102793806576399No Hit
GTCTGGGACCCTGGCCAGGTTTCAGTTGGTACCAGGCTAAGTCGTTGCGAACACTCTGACTGGCCCTGCAGGAGA20770.19093600931789786No Hit
GTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAACGACTTAGCCTGGTA20550.1889135768648436No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAA20320.1867992156639232No Hit
GACTTCACTCTCACCATCAGCCGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCACCAGCGTAGCAACTG20020.18404135322794007No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA19990.1837655669843418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19130.17585969466785684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18360.16878118108216683No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAACGACTTAGCCTGGTACCAACTGAAACCTGGCCAGGGTCCCAGACT18090.16629910488978203No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT17430.16023180753061916No Hit
GGCCAAAACTGTACCTCCGAGGCCAGTTGCTACGCTGGTGACAGTAATAAATTGCAAAATCTTCAGGCTCTAGGC17290.15894480506049372No Hit
GTCTGGGACGGACTTCACTCTCACCATCAGCCGCCTAGAGCCTGAAGATT17160.15774973133823436No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16230.1492003577866867No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15770.14497163538484592No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA15710.1444200628976493No Hit
TCTATGAAGCATCCAACAGGGCCCCTGGCACCCCAGCCAGGTTCAGCGGCGGTGGGTCTGGGACGGACTTCACT15550.1429492029317916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15220.1399155542522102No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCTCCGAG14980.1377092643034237No Hit
GAGTGAAGTCCGTCCCAGACCCACCGCCGCTGAACCTGGCTGGGGTGCCAGGGGCCCTGTTGGATGCTTCATA14940.1373415493119593No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCTCCGAGGCCAGTTGCTAC14670.1348594731195745No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14670.1348594731195745No Hit
GTATTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCA14520.13348054190158293No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13960.1283325320210811No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13820.12704552955095566No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13620.12520695459363357No Hit
GTCAGAGTGTTCGCAACGACTTAGCCTGGTACCAACTGAAACCTGGCCAGGGTCCCAGACTCCTCATCTATGAA13420.12336837963631149No Hit
GCGTAGCAACTGGCCTCGGAGGTACAGTTTTGGCCAGGGGACCAAGCTGG13020.11969122972166732No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12950.1190477284866046No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12690.11665758104208591No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12350.11353200361463836No Hit
GACTTAGCCTGGTACCAACTGAAACCTGGCCAGGGTCCCAGACTCCTCATCTATGAAGCATCCAACAGGGCCC12220.11233692989237902No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12050.11077414117865525No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11940.1097629249521281No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11940.1097629249521281No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11850.10893556622133317No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11670.1072808487597433No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG11470.10544227380242122No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTATTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA11400.10479877256735848No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11280.10369562759296524No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11240.10332791260150083No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11120.10222476762710758No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11010.10121355140058044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT1751.5151054E-647.37526770
TAGGCAA6600.041.8720870
GTATCAA37600.040.702451
GGTATCA14150.040.4990731
AATGGTA2453.374007E-739.4793970
GTACGGT451.1479609E-437.4550251
TATTCTA1600.002576420334.54446870
TATCAAC45150.033.7018552
ATCAACG45300.033.6568373
GCGGGAA17350.033.44939870
TCAACGC45750.033.255284
AACGCAG45900.033.1466036
CAACGCA46200.032.9313625
TGGACGT2102.4553246E-432.8994970
CGGGCGT1700.003467785432.5124470
CGCAGAG48250.031.4624448
ACGCAGA48550.031.3373627
AGAGTAC48700.031.31715611
GCAGAGT49050.031.017929
AAAGTCA4101.8097126E-830.33172670
Produced by FastQC (version 0.11.5)