FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694096
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCGTCATCTCTCAGGCTGTTCATGTACAGATACA20130.290017519190429No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG16910.2436262419031373No Hit
GCATGGGACAGCAGCCTCAGTGCCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG15630.22518498881999033No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG15570.22432055508171783No Hit
CACCTGCACTGGGAACAGAAACAATGTTGGCAACCAAGGAGCAGCTTGGCTGCAGCAACACCAGGGCCACCCTCC15240.21956616952121896No Hit
GGGCTAGAGTGGGTGGCAGTTCTATCATCTGATCGAAGTTATGAACACTA15190.21884580807265855No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14810.21337106106359927No Hit
ATATATTACTGTACGAGAGATCTGGCCAACACAGAGTACTATTTTGACTA14630.21077775984878175No Hit
GACTATTACTGCTCAGCATGGGACAGCAGCCTCAGTGCCGTGGTTTTCGG14430.20789631405454No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGCACTGAGGCTGCTGTCCCA14110.20328600078375328No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC13290.19147207302736222No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12860.18527696456974252No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC12520.1803785067195316No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11860.1708697355985339No Hit
GTGTTGGCCAGATCTCTCGTACAGTAATATATAGCCGTGTCGTCATCTCTCAGGCTGTTCATGTACAGATACAG11390.16409833798206588No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCAGTGCCGTGGTTT10990.15833544639358244No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG10740.1547336391507803No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10650.1534369885433715No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10580.1524284825153869No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT10530.1517081210668265No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGCACTGAGG10470.15084368732855397No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10430.15026739816970563No Hit
GTACTATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCT10090.1453689403194947No Hit
GTTCATGTACAGATACAGTGTGTTTTTGGAATTGTCTCTGGAGATGGTGA9920.14291971139438925No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9850.14191120536640464No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9790.14104677162813212No Hit
CTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAAAACACACTGTATCTGTACATGAA9570.13787718125446624No Hit
CTCCTATCCTACAGGAATGACAACCGGCCCTCGGAGATCTCAGAGAGATT9380.1351398077499366No Hit
GTACATGAACAGCCTGAGAGATGACGACACGGCTATATATTACTGTACGAGAGATCTGGCCAACACAGAGTACTA9310.134131301721952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9280.13369908485281573No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9110.1312498559277103No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9100.1311057836379982No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC9020.1299532053203015No Hit
GTATCTGTACATGAACAGCCTGAGAGATGACGACACGGCTATATATTACT9010.12980913303058944No Hit
AAATAGTACTCTGTGTTGGCCAGATCTCTCGTACAGTAATATATAGCCGTGTCGTCATCTCTCAGGCTGTTCATG8850.12750397639519603No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8720.12563103662893893No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8700.12534289204951476No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCAGTTCTATCATCTGATCGAAGTTA8650.12462253060095434No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8610.12404624144210599No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8500.12246144625527304No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGAAACAATGTTGGCAACCAAGGAGCAGCTTGGC8500.12246144625527304No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8330.12001221733016758No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG8160.11756298840506212No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8090.11655448237707752No Hit
CCCAAACTCCTATCCTACAGGAATGACAACCGGCCCTCGGAGATCTCAGA8080.11641041008736543No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8000.11525783176966875No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACTTGGATGCAGAGAATCTCTCTGAGATCTCCGAGGGCCGGT7900.1138171088725479No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA7880.11352896429312372No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7850.11309674742398747No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7660.11035937391945783No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7630.10992715705032156No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7580.10920679560176114No Hit
GAGTACTATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGG7530.10848643415320072No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7470.1076220004149282No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAATAGTAC7450.10733385583550402No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7440.10718978354579195No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC7430.10704571125607985No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7430.10704571125607985No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGAAACAATGTTGGCAACCAAGGAGCAG7370.10618127751780733No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7330.10560498835895898No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7240.10430833775155021No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG7060.10171503653673268No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7010.10099467508817223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1900.073.22172570
CTTAACG150.002269099269.6505758
TAAGACG200.0077026351.1539545
GTGGTAT1600.046.9114071
CGATTCA7400.044.31469370
AATGGTA1807.994458E-944.1654970
CGGGCGA1352.4549936E-644.1654970
GGTATCA6000.043.7839781
TGGTATC1700.042.1267852
TGCACAG7850.041.77436470
TCGTATG1500.038.85401540
GTATCAA14550.037.048431
CGTATGC1600.036.44676241
TATACAC1600.036.234053
GTCACCG9900.036.135470
ACGAGAC1750.035.08205422
ATGCCGT1700.034.3875744
GCTACAT1500.034.1691132
CGAGACG1700.034.1100123
ACGCCAT400.002747330834.1026346
Produced by FastQC (version 0.11.5)