FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694096
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19040.27431363961181165No Hit
GTAATATATAGCCGTGTCGTCATCTCTCAGGCTGTTCATGTACAGATACAGTGTGTTTTTGGAATTGTCTCTGGA17520.2524146515755746No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT15070.2171169405961135No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACTTGGATGCAGA14780.21293884419446302No Hit
GGGCTAGAGTGGGTGGCAGTTCTATCATCTGATCGAAGTTATGAACACTA14260.20544708512943455No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13920.20054862727922362No Hit
CACCTGCACTGGGAACAGAAACAATGTTGGCAACCAAGGAGCAGCTTGGCTGCAGCAACACCAGGGCCACCCTCC13760.19824347064383024No Hit
GCATGGGACAGCAGCCTCAGTGCCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG13110.18887877181254464No Hit
GTCCCATGCTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC13100.1887346995228326No Hit
GACTATTACTGCTCAGCATGGGACAGCAGCCTCAGTGCCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA13080.1884465549434084No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGCACTGAGGCTGCTGTCCCAT12800.18441253083147No Hit
ATATATTACTGTACGAGAGATCTGGCCAACACAGAGTACTATTTTGACTACTGGGGCCAGGGAACCCTGGTCACC12650.1822514464857887No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTACTGAAGGTGAATCCAGAGGCTGCACA11860.1708697355985339No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGG11150.16064060302897581No Hit
GTGTTGGCCAGATCTCTCGTACAGTAATATATAGCCGTGTCGTCATCTCTCAGGCTGTTCATGTACAGATACAGT11150.16064060302897581No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10450.1505555427491298No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG10120.145801157188631No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10100.1455130126092068No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9920.14291971139438925No Hit
CTCCTATCCTACAGGAATGACAACCGGCCCTCGGAGATCTCAGAGAGATTCTCTGCATCCAAGTCAGGAAACACA9860.14205527765611675No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA9820.1414789884972684No Hit
GTTCATGTACAGATACAGTGTGTTTTTGGAATTGTCTCTGGAGATGGTGAATCGGCCCTTCACGGAGTCTGCGTA9770.14075862704870795No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9720.14003826560014754No Hit
GTACTATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT9680.13946197644129918No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG9540.13744496438533No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA9230.13297872340425532No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9230.13297872340425532No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9060.13052949447914985No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC9030.1300972776100136No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8910.12836841013346856No Hit
AAATAGTACTCTGTGTTGGCCAGATCTCTCGTACAGTAATATATAGCCGT8890.1280802655540444No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8820.12707175952605979No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8770.12635139807749937No Hit
GTACATGAACAGCCTGAGAGATGACGACACGGCTATATATTACTGTACGA8750.1260632534980752No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGCACTGAGG8590.12375809686268181No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8530.12289366312440929No Hit
GTATCTGTACATGAACAGCCTGAGAGATGACGACACGGCTATATATTACTGTACGAGAGATCTGGCCAACACAGA8290.11943592817131923No Hit
CTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAAAACACACTGTATCTGTACATGAA8290.11943592817131923No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCACTGGGAACAGAAACAATGTTGGCAACCAAGGAGCAGCTTGGCT8020.11554597634909293No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA7970.11482561490053249No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7960.1146815426108204No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACTTGGATGCAGAGAAT7870.11338489200341165No Hit
GAGTACTATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT7840.11295267513427537No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA7780.11208824139600285No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCAGTTCTATCATCTGATCGAAGTTAT7730.11136787994744243No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7480.10776607270464027No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7310.1053168437795348No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAATAGTACT7220.10402019317212605No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7070.10185910882644475No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7060.10171503653673268No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC7020.10113874737788434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)