FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805981
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT41850.519243009450595No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT21390.26539087149697077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20630.2559613688163865No Hit
GATATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAAGAACTCACTGTATC19450.24132082518074247No Hit
GAGTTATGGTAGTCCGCGTGGGGCGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCA17960.2228340370306496No Hit
TCCTTATACCAGCCACTGCCCCCCACCTCTCTCGCACAGAAATACACGGC17120.21241195512053013No Hit
GTATAAGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCAT16660.20670462455070282No Hit
GTATATATCATCACCACTACCACTCATGAATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGC15570.19318073254828588No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15180.18834190880430182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14850.18424751948246917No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG14060.17444579959080922No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12980.1610459799920842No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12730.1579441698997867No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12390.15372570817426218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12260.1521127669262675No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11670.1447924951084455No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11620.144172133089986No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTCGCAGTCATTTAAATTGGTATCAACAGAGA11480.14243511943829942No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11410.14156661261245612No Hit
GAAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA11350.14082217819030474No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10980.13623149925370448No Hit
ACCATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAA10940.1357352096389369No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10820.1342463407946341No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10700.13275747195033133No Hit
GTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTCGCAG10660.13226118233556375No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTC10650.13213710993187183No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10630.13188896512448806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10450.12965566185803387No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10280.1275464309952716No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10140.12580941734358503No Hit
CTACTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATTCATGAGTGGTAGTGGTGA10120.12556127253620122No Hit
GTTATGGTAGTCCGCGTGGGGCGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCA9960.12357611407713086No Hit
GCACTGAACACAGAGGACTCACCATGAAGTTTGGGCTGAGCTGGGTTTTC9870.12245946244390377No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT9720.12059837638852529No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9550.11848914552576302No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCACTGACTACTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG9380.11637991466300075No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9320.11563548024084934No Hit
CTCCAGGGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTGTATT9290.11526326302977366No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9270.11501511822238986No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9240.11464290101131416No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA9180.11389846658916276No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9140.11340217697439518No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC9090.11278181495593569No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8970.1112929461116329No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8860.10992814967102202No Hit
GACTACCATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAA8700.10794299121195164No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8610.10682633957872457No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC8580.10645412236764885No Hit
CCATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC8450.10484118111965418No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8440.10471710871596228No Hit
ATGGTAGTCCGCGTGGGGCGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCT8370.103848601890119No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA8360.1037245294864271No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG8310.10310416746796762No Hit
GTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCCCTTGGCCGAACGCCCCACGCGGACTACCATAACTCTGT8260.10248380544950811No Hit
ACAGAGACCAGGGCAAGCCCCTAAACTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGT8170.10136715381628104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA252.0103827E-4128.1419570
CCGCATA250.00215831270.5313869
AGGGGGG13850.067.0779270
ATCGCGT750.054.23755637
AATGGTA2600.053.39248370
CGCGTAT653.6379788E-1252.5786939
AACGGGG453.3662445E-452.24547269
CGAATCG850.051.37471834
AATCGCG800.050.70174436
AAAGGGG7900.049.32174368
TATTGGG905.4023636E-447.45998470
CGTATGC7400.046.98219341
ATGGGGG2052.564775E-1046.88120370
TGTTAGA6650.046.56787570
GTATGCC8000.046.1842442
ATGCCGT7450.046.17776544
CGTGAAA950.046.0986157
TCGTATG7200.045.73930740
GCGAATC8500.045.3261833
GGCGAAT9300.045.2638332
Produced by FastQC (version 0.11.5)