FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805981
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95871.1894821341942303No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21250.26365385784528417No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA19970.2477725901727212No Hit
TCCTTATACCAGCCACTGCCCCCCACCTCTCTCGCACAGAAATACACGGC18890.23437277057399616No Hit
GATATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAAGAACTCACTGTATCT16220.20124543878825926No Hit
GAGTTATGGTAGTCCGCGTGGGGCGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT15960.19801955629226992No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14990.18598453313415578No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14580.1808975645827879No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14580.1808975645827879No Hit
GTATATATCATCACCACTACCACTCATGAATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT14510.18002905775694464No Hit
GTATAAGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCC13120.16278299364377077No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA12940.1605496903773166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC12530.15546272182594875No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTCGCAGTCATTTAAATTGGTATCAACAGA11730.14553692953059688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11180.13871294732754247No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG10990.13635557165739637No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10820.1342463407946341No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10440.12953158945434198No Hit
ACCATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT10390.12891122743588249No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10390.12891122743588249No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10340.128290865417423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC10270.12742235859157972No Hit
GTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACAGAGTCACCATCACTT10250.12717421378419588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC9990.12394833128820655No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA9850.12221131763651998No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9810.12171502802175238No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCACTGACTACTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG9730.12072244879221719No Hit
CTACTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATTCATGAGTGGTAGTGGT9630.1194817247552982No Hit
GAAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCCCTGGA9570.11873729033314681No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9390.11650398706669264No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTCACTGACTACTATATGAGCTGGGTCC9230.11451882860762228No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT9030.11203738053378429No Hit
GCACTGAACACAGAGGACTCACCATGAAGTTTGGGCTGAGCTGGGTTTTC9030.11203738053378429No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9020.1119133081300924No Hit
CTCCAGGGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTGTATTT9000.1116651633227086No Hit
GTTATGGTAGTCCGCGTGGGGCGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT8960.11116887370794101No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8870.11005222207471392No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8700.10794299121195164No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8530.10583376034918937No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA8500.10546154313811368No Hit
ATGGTAGTCCGCGTGGGGCGTTCGGCCAAGGGACCAAGGTGGAAAGCAAA8460.1049652535233461No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGCCTGCATCTGTA8280.1027319502568919No Hit
GACTACCATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT8280.1027319502568919No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8170.10136715381628104No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8140.10099493660520532No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8120.10074679179782153No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8100.10049864699043774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGT1203.778554E-666.6745670
AGTCCTA1101.9733694E-458.188770
CTCCGCA900.006519354453.3396470
TTGTTAA900.006519354453.3396470
TAGTCGT200.00845808549.9579931
AAAGTCA2001.3800054E-648.0056870
AATGGTA2101.9312592E-645.71969670
GAGCAAA12700.044.0997170
AGAGCAA2404.8382535E-640.00473470
CCAGAGT5300.039.24992870
CGTTATT350.001543676238.3534234
GAGCCAT8300.036.6308470
CTGTCTT12350.036.279670
TTCTTAA2251.55238E-435.55976570
GTCGGAA1900.002910629633.68819870
GTGGTAT3100.032.8047751
CCGAAAT552.5929767E-432.60471352
TGGAGTT17800.032.3633870
TCTTCAA2000.00374211632.00378870
TGAGCCA2553.237401E-431.3762670
Produced by FastQC (version 0.11.5)