FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127881
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTG53580.47505011610267395No Hit
GTATAGTAGTAGTGCTGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC51870.4598889421845035No Hit
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGAAGATACA51370.45545585039556474No Hit
CCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG43890.38913679723304145No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG43610.38665426583123574No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCACTACTACTATACCACAC41260.3658187344232237No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37850.33558504842266157No Hit
GGATTACCTGTGGGGAAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC36200.32095584551916384No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCACTA35470.3144835315073133No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACAT32860.29134279236905314No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG31360.27804351700223695No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31010.27494035274997985No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTC28520.252863555641065No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACATTGGAAGT28210.250115038731923No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTTCCAATGTTGTTTTCCCCACAGGTAA27270.24178082616871815No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT26740.2370817488724431No Hit
GTCTATTACTGTGTGAAAGATGGAATTATAGCAGTGGCTGGTACCGAGTT25690.22777225611567176No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25090.2224525459689453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24090.21358636239106782No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG23630.2095079179452442No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGAC23530.20862129958745648No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22520.19966645417380025No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC22510.1995777923380215No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22310.197804555622446No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22090.19585399523531297No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21980.19487871504174642No Hit
GGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC21540.19097759426748034No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21430.19000231407391382No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20530.1820227488538241No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20040.17767831890066416No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20000.17732367155754908No Hit
GTCTGAGACCTGAGGACACGGCTGTCTATTACTGTGTGAAAGATGGAATTATAGCAGTGGCTGGTACCGAGTT19620.17395452179795565No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC19570.17351121261906177No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19520.1730679034401679No Hit
GCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGA19410.17209262324660138No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG18880.16739354595032632No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG18870.16730488411454755No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA18850.16712756044299No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCAC18730.1660636184136447No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18300.1622511594751574No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG17950.1591479952229003No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTG17730.15719743483576723No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA17700.15693144932843092No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGT17580.15586750729908563No Hit
CTTTTACTTCCAATGTTGTTTTCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGG17230.1527643430468285No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17050.15116843000281058No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG16630.14744463290010207No Hit
CAGTAATAGACAGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTC16600.14717864739276573No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT16520.14646935270653552No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT16500.14629202903497798No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16360.14505076333407513No Hit
CTATTACTGTCAGGTGTGGTATAGTAGTAGTGCTGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG16140.14310020294694212No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG16140.14310020294694212No Hit
GTAGTAGTGCTGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGG15950.14141562806714536No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCACTAC15830.1403516860378001No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT15730.13946506768001235No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG15640.13866711115800337No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15570.13804647830755196No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15300.13565260874152504No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15230.1350319758910736No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACATTGGAAGTAAAAGTG15160.1344113430406222No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14950.13254944448926792No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACA14840.1315741642957014No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14840.1315741642957014No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14780.13104219328102876No Hit
ATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC14540.12891430922233818No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14470.12829367637188677No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14470.12829367637188677No Hit
CTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG14210.12598846864163862No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA14020.12430389376184191No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGC13990.12403790825450556No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG13870.12297396622516028No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13850.12279664255360273No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC13760.12199868603159375No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA13720.12164403868847866No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC13520.11987080197290317No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCT13450.11925016912245175No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCA13360.11845221260044278No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG13350.118363550764664No Hit
CATCAGCACTACTACTATACCACACCTGACAGTAATAGTCGGCCTCATCC13200.11703362322798237No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13030.11552637201974322No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12840.11384179713994651No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12830.11375313530416772No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12710.11268919327482242No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACATTGGAAGTAAAAGTGTGCACTGG12700.11260053143904367No Hit
CTACTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC12640.11206856042437101No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12620.11189123675281346No Hit
GTATGTGCTACCCCCATTACTACTAATACCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAG12590.11162525124547715No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12560.11135926573814081No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG12470.11056130921613183No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT12460.11047264738035308No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC12260.10869941066477759No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12220.10834476332166247No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12140.10763546863543229No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12090.10719215945653841No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12090.10719215945653841No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC12010.10648286477030822No Hit
GCACTACTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGG11860.1051529372336266No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11850.10506427539784782No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11830.10488695172629027No Hit
AAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC11820.10479828989051151No Hit
GGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG11820.10479828989051151No Hit
CTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGT11810.10470962805473273No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAAGAAACTCGG11810.10470962805473273No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11750.10417765704006007No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11710.10382300969694498No Hit
GCTTGGTCCCTCCGCCGAATACCACATCAGCACTACTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCC11580.1026704058318209No Hit
CAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACAGAGGAGGTCACAGAG11520.10213843481714827No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC11310.100276536265794No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG11290.10009921259423644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)