FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127881
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC52220.46299210643676064No Hit
GTATAGTAGTAGTGCTGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC51210.4540372610231044No Hit
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTGGA43890.38913679723304145No Hit
CCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG42750.37902934795426113No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT41880.37131576824150775No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCACTACTACTATACCACAC38720.343298628135415No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36450.3231723914136332No Hit
GGATTACCTGTGGGGAAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC34240.30357812570652404No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCACTA32460.28779631893790214No Hit
GTCTATTACTGTGTGAAAGATGGAATTATAGCAGTGGCTGGTACCGAGTTTCTTTTTGATATCTGGGGCCAAGG32080.28442716917830874No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACATT32000.28371787449207847No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29640.2627936812482877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27990.24816447834478994No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG27820.24665722713655075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26820.23779104355867328No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACATTGGAAGT26700.236727101529328No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT26500.2349538648137525No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26010.23060943486059257No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24040.21314305321217397No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACT23350.20702538654343855No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA22800.20214898557560593No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATA22790.20206032373982719No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22680.20108504354626064No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGT22390.1985138503086762No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC21740.19275083098305584No Hit
GGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC21330.18911569571612608No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG21170.18769710634366568No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACA21150.18751978267210814No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20900.18530323677763877No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20670.18326401455472696No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20400.18087014498870005No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG20220.17927423194468212No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG19440.1723586087539377No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19330.17138332856037117No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCG18970.1681915024723353No Hit
GCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG18720.16597495657786593No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTC18570.1646450290411843No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG18420.16331510150450268No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG18390.16304911599716637No Hit
GTCTGAGACCTGAGGACACGGCTGTCTATTACTGTGTGAAAGATGGAATTATAGCAGTGGCTGGTACCGAGTTT18260.16189651213204231No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT18090.16038926092380312No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17790.1577294058504399No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT17500.15515821261285542No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG17490.15506955077707665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT17390.1541829324192889No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG17280.15320765222572238No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG17210.15258701937527097No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16750.14850857492944733No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG16490.1462033671991992No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCA16450.1458487198560841No Hit
CTATTACTGTCAGGTGTGGTATAGTAGTAGTGCTGATGTGGTATTCGGCG16400.14540541067719023No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16120.14292287927538455No Hit
CTTTTACTTCCAATGTTGTTTTCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTG15310.1357412705773038No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGC15210.13485465221951606No Hit
GTAGTAGTGCTGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA15040.1333474010112769No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC14810.1313081787883651No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCACTACTACTATACCACACCTGACAGTAATA14800.13121951695258632No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14750.13077620777369245No Hit
CAGTAATAGACAGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTG14750.13077620777369245No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14750.13077620777369245No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGTATAGTAGTA14600.12944628023701082No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGG14540.12891430922233818No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14510.12864832371500184No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT14290.1266977633278688No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGA14090.12492452661229331No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAT14000.12412657009028434No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT13910.12332861356827537No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAACATTGGAAGTAAAAGTGT13860.1228853043893815No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCAGCA13780.12217600970315132No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTT13630.12084608216646969No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13560.12022544931601827No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC13440.11916150728667298No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13400.11880685994355787No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG13370.11854087443622155No Hit
CTCACTGCACAGGCTCTGTGACCTCCTCTGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG13260.11756559424265503No Hit
ATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC13000.1152603865124069No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG12840.11384179713994651No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12760.1131325024537163No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGAAAACAA12700.11260053143904367No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12310.10914271984367147No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG12100.1072808212923172No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC12100.1072808212923172No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT12020.10657152660608699No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11870.10524159906940539No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT11870.10524159906940539No Hit
GCTTGGTCCCTCCGCCGAATACCACATCAGCACTACTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCC11820.10479828989051151No Hit
GTATGTGCTACCCCCATTACTACTAATACCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGT11790.10453230438317518No Hit
GGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGC11750.10417765704006007No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC11710.10382300969694498No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA11690.10364568602538743No Hit
GTGTGGTATAGTAGTAGTGCTGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG11640.10320237684649355No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC11580.1026704058318209No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG11570.10258174399604214No Hit
CATCAGCACTACTACTATACCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCA11570.10258174399604214No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA11570.10258174399604214No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA11560.10249308216026337No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11550.10240442032448459No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11440.10142914013091806No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11400.10107449278780296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGTA700.003250213663.6031570
AGGTAAT18900.055.75090870
AATGGTA2151.1059456E-955.2213470
GTAGTTT1351.1893206E-554.96568770
TATTCTA1502.2186678E-549.46911270
GCCGTGT38900.047.30722470
AAAACAA32600.041.4265970
GGTAGAA25900.041.25609670
ACTCGGT8550.039.92244370
TGGTATC2650.039.707692
AGGGACA6750.039.57529470
TAGGCAA1500.001321526139.5752970
GGTATCA7700.038.8323671
AGAGTCG350.001458647538.7996511
ATGTCCA11000.037.77641770
GCTTGGG1350.037.753691
CACAGTA7150.037.3612970
TATAAGA656.1912397E-736.554722
GTATCAA17500.036.308261
AGTGGAA13900.035.233470
Produced by FastQC (version 0.11.5)