FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190902
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTTGGTCATCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG80960.6798208416813475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT53400.448399616425197No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA51240.43026210385069474No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48860.410277252032493No Hit
GTGTATTACTGTCAGCAGTATGGTTGGTCATCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA37940.3185820495725089No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA36590.30724610421344495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34170.2869253725327525No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT33390.2803757152141822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33000.277100886554897No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32360.2717268087550445No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA30460.2557725152867322No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29920.25123813714310667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29410.24695566889634918No Hit
GTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCA29020.24368084023706402No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA28790.241749531027742No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27820.23360444436234049No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27480.2307494655311688No Hit
GAGTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG27140.22789448669999715No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC27130.22781051673437447No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26660.22386392835010774No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC26590.22327613859074885No Hit
GTCCCAGACCCACTGCCACTAAACCTGTCTGGGATGCCAGTGGTCCTGCTGGATGCACCATAGATGAGGAGTC24420.2050546560506238No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTAAACCTGTCTGGGATGCCAGTGGTCCTGCTGGATGCACCATAGA23990.2014439475288479No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA23800.19984851818201665No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGTGACTACTTAGCC23010.19321489089782368No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22580.18960418237604773No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21800.18305452505747746No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA21050.17675677763577524No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21050.17675677763577524No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG20950.17591707797954828No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG20230.16987124045471416No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20120.1689475708328645No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTTGGTCA19870.1668483216922971No Hit
GTCAGAGTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTA19720.16558877220795665No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGTGACTACTTAGCCTGGTACC19670.16516892237984318No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19570.16432922272361622No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA18980.15937499475187716No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18880.15853529509565017No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGACCACTGGCATCCCAGACAGGTTTAGTGGCAGTGGGTC18730.15727574561130975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18430.15475664664262886No Hit
GTATGGTTGGTCATCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA17370.14585583028662308No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA17060.14325276135231949No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.14266497159296063No Hit
GTCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTAGTCACTG16940.14224512176484713No Hit
CATCTATGGTGCATCCAGCAGGACCACTGGCATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCA16810.1411535122117521No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16730.14048175248677053No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16560.13905426307118468No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACGCAGTCTC16490.13846647331182582No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16300.1368710439649946No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTAGTCACTGGTAACACTCTGA15600.13099314637140588No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15310.12855801736834768No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15180.12746640781525265No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15150.12721449791838454No Hit
GCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGACCACTGGCATCCCAGACAGGTT14740.123771729327854No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14490.1216724801872866No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14430.12116866039355043No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14230.1194892610810965No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14190.11915338121860573No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG14160.11890147132173765No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACGCAGTC14130.11864956142486956No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14050.11797780169988797No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13880.11655031228430215No Hit
CTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGACCA13810.1159625225249433No Hit
CACTAAACCTGTCTGGGATGCCAGTGGTCCTGCTGGATGCACCATAGATGAGGAGTCTGGGAGCCTGGCCAGGCT13810.1159625225249433No Hit
CTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA13800.11587855255932057No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13620.11436709317811207No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13590.11411518328124397No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13560.11386327338437588No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13500.1133594535906397No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13360.11218387407192194No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13360.11218387407192194No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13260.111344174415695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG13240.11117623448444959No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13090.10991668500010916No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12910.10840522561890063No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12820.10764949592829637No Hit
GTGTATTACTGTGCGAGAGATGGTGACTTCGGTGACTACCGGGCGTACTTCCTGCACTGGGGCCAGGGCACCC12780.10731361606580558No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT12700.10664185634082401No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12650.10622200651271053No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12390.10403878740652044No Hit
GGCTTCTGCTGGTACCAGGCTAAGTAGTCACTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC12360.10378687750965233No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12350.10370290754402964No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12080.10143571847221686No Hit
CAGCAGGACCACTGGCATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGA11970.1005120488503672No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC11920.1000921990222537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTA2300.049.4886170
TGTTAGA13050.045.98941470
GGTATCA11000.044.92861
GTAGATA1951.1374141E-842.45176770
GGTACGG405.6069654E-542.298683
GACCGGA1256.364779E-541.39047270
GTGGTAT2650.039.598481
TCGTATG1750.039.18139340
AATGGTA4000.038.8035770
CGTTATA350.001467668738.75117527
CAATACG350.001481985938.6747064
GTATCAA27850.038.6513441
CGTATGC1800.038.13844741
GCTTGTA1100.036.9276161
GTATTAT656.3265907E-736.4541851
TGGTATC3000.036.0948752
CGTCGGA2304.8747097E-835.9917270
AATCTCG1900.035.9276636
CGTAGAG2050.034.7518328
ATCTCGT2000.034.16600437
Produced by FastQC (version 0.11.5)