FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190902
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTTGGTCATCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT75100.6306144418264474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52810.443445388453458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA47750.4009565858483738No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA47200.3963382377391255No Hit
GTGTATTACTGTCAGCAGTATGGTTGGTCATCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA36470.3062384646259726No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA35550.29851322778868455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32630.2739939978268573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT31980.26853595006138203No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29930.2513221071087294No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC29890.2509862272462386No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA28820.2420014409246101No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA28550.2397342518527973No Hit
GTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCAT27860.23394032422483124No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27190.22831433652811062No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA26810.22512347783444817No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26720.2243677481438439No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26490.22243643893452192No Hit
GAGTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG26240.22033718979395453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25680.21563487171908352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT24820.20841345467553166No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCA24220.20337525673816986No Hit
GTCCCAGACCCACTGCCACTAAACCTGTCTGGGATGCCAGTGGTCCTGCTGGATGCACCATAGATGAGGAGTCTG23980.2013599775632252No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGTGACTACTTAGCCTG23490.1972454492477131No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACT23410.19657368952273152No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23360.19615383969461805No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTAAACCTGTCTGGGATGCCAGTGGTCCTGCTGGATGCACCATA22450.1885125728229527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21700.18221482540125047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21170.1777644172232476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21020.17650486773890714No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGT20670.1735659189421128No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20140.16911551076410988No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20090.16869566093599642No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA19770.16600862203607014No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA19590.1644971626548616No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA19570.16432922272361622No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18730.15727574561130975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18580.1560161961269693No Hit
GTCAGAGTGTTACCAGTGACTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATG18380.15433679681451537No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA18180.15265739750206148No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18180.15265739750206148No Hit
GTCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTAGTCACTGGTAACACTCTGACTGGCCCTGCAG17990.15106196815523024No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGACCACTGGCATCCCAGACAGGTTTAGTGGCAGTGGGTCTG17360.1457718603210004No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17230.14468025076790533No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16480.13838250334620314No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16290.1367870739993719No Hit
GTATGGTTGGTCATCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16040.1346878248588045No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGTAGTCACTGGTAACACTCT15970.13410003509944562No Hit
GCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCAGCA15890.13342827537446408No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15530.130405356612047No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA15340.12880992726521578No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15150.12721449791838454No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACGCAGTCTC15100.12679464809027108No Hit
CATCTATGGTGCATCCAGCAGGACCACTGGCATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCA15000.1259549484340441No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14920.12528318870906255No Hit
GGTTTAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGT14840.12461142898408098No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14640.12293202967162706No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14630.12284805970600436No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA14340.12041293070294617No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13930.11697016211241562No Hit
CTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGA13850.11629840238743405No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13760.1155426726968298No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13470.11310754369377161No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13450.11293960376252622No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13380.11235181400316735No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13130.11025256486259995No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12960.10882507544701411No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA12840.10781743585954176No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12740.10697773620331481No Hit
CTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGACCA12670.10638994644395591No Hit
CACTAAACCTGTCTGGGATGCCAGTGGTCCTGCTGGATGCACCATAGATG12470.104710547131502No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC12450.10454260720025661No Hit
CAGCAGGACCACTGGCATCCCAGACAGGTTTAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCA12300.10328305771591619No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT12260.10294717785342539No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12120.10177159833470766No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11990.1006799887816126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA652.1642883E-590.9296470
GCGATTT508.702275E-488.65639570
AATGGTA3750.074.865470
TAGGCAA3850.049.8932170
GAGCAAA25750.041.8894370
TAGAATT2552.7293208E-740.5617570
GCCACTA21450.039.95393470
GCCGGTT3351.3078534E-939.69689670
GAACTGT26300.038.51376369
TGACCAA18700.037.92786870
GGTATCA11600.037.861161
GTATCAA27350.037.4277571
TGGTATC3900.036.3550532
GTAGATA2459.654006E-636.18628370
CGGCAAA1650.002156467235.82076670
GTGGTAT4050.035.0350531
TATTCTA2651.6525004E-533.45524270
AAACACA27000.033.38296570
AATCGAA2701.8779583E-532.835770
ATCAACG32800.031.5876263
Produced by FastQC (version 0.11.5)