FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352922
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGGTCATTTGGAGATAGA73460.5429729134421645No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT50140.37060525292662844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44660.3301003309873001No Hit
GCGTAAATACTGGCCTCCGCTCATTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA42960.3175349354951727No Hit
GTCTATTACTGTGCGAGAGATCAAGAGGGTACCAGCAACCGCTACGCCCGCTTTGACTCCTGGGGCCAGGGAACC40110.2964694195230767No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36530.270008174898479No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35680.2637254771524153No Hit
GTATTTACGCTGCTGACAGTAGTAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAG34080.2518992225715895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT32320.2388903425326811No Hit
GTCTCCAGCCACCCTGTCTTTGGCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACA30890.22832062750106807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30520.22558580612925208No Hit
GTCTTTGGCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACATCTACTTAGCCTGGTA29530.21826831110736614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29040.21464652064198822No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC27830.2057029156152387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27800.20548117334184823No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGCG26160.19335926239650178No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC26150.19328534830537164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25470.18825919010852069No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGC25460.1881852760173905No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25380.1875939632883492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25010.18485914191653324No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24330.1798329837196823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24010.17746773280351713No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23280.17207200415101537No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC22740.16808064322998664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22700.167784986865466No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGCGTAAATAC22560.16675018958964374No Hit
GTTTACTACTGTCAGCAGCGTAAATACTGGCCTCCGCTCATTTTCGGCGG22130.16357188367104683No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22110.1634240554887865No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21380.1580283268362847No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21360.1578804986540244No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA21240.15699352956046247No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA21100.15595873228464022No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGGCTCCAGGGGAGAGAGC20580.15211519954587183No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20380.1506369177232686No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20220.14945429226518603No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGCGTAAATAC20180.14915863590066536No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20080.14841949498936377No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAATGAGCGGAGGCCAGTATTTACGC19670.14538901725302716No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18000.13304536403429024No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17980.1328975358520299No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17600.13008880038908377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16860.12461915764545184No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16660.1231408758228486No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC16400.12121910945346442No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC16290.12040605445103264No Hit
GCACTGAACACAGAGGACTCACCATGGACTTGGGGCTGTGCTGGGTTTTC16090.11892777262842943No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16040.11855820217277861No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15920.11767123307921667No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT15770.11656252171226428No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15700.11604512307435313No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTC15480.1144190130694896No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15470.11434509897835943No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15290.11301464533801653No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15050.11124070715089265No Hit
CAGTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGGTCATTTGGAGATAGAGTGAGTTCTTGGCATTGTCTC14860.10983633941941959No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14480.10702760395647347No Hit
ACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT14240.1052536657693496No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14190.10488409531369879No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14190.10488409531369879No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14170.10473626713143848No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13930.1029623289443146No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13660.10096664848380024No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13620.1006709921192796No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13540.1000796793902383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG18300.075.5103470
TGTTAGA12350.048.2977670
TCAAGCG907.6922664E-444.18350670
GACAGGT2550.043.767171
ACATCGA1154.9308386E-543.22299670
CTTATAA950.001003794341.8580670
TAAGTCA1454.010024E-641.13636870
GGTATCA9650.041.004661
TCGTATG2050.040.1283840
TCCGAGA12500.039.7651670
AATGGTA2755.456968E-1239.76515670
TATCGGG350.001418290539.02232736
GATGGAG9600.038.31538470
ACCGATC3100.038.30434827
GTATCAA23900.038.206791
GACCGAT3200.038.13925626
CGTATGC2250.038.12623641
GCCGTCT2050.037.03414546
GTGAGCG6450.036.99084570
AGTATCG656.022583E-736.6847734
Produced by FastQC (version 0.11.5)