FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352922
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGGTCATTTGGAGATAGAGTGAGTTCTTGGCATTGTCTCTGGA58420.4318061203824019No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50360.37223136293149195No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA43610.3223393514186332No Hit
GTATTTACGCTGCTGACAGTAGTAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT43220.3194567018645569No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42460.3138392309386646No Hit
GCGTAAATACTGGCCTCCGCTCATTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT36760.2717081989944727No Hit
GTCTATTACTGTGCGAGAGATCAAGAGGGTACCAGCAACCGCTACGCCCGCTTTGACTCCTGGGGCCAGGGAACC36580.2703777453541298No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG36230.26779075216457415No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA35270.2606949994160787No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT31640.23386418433583014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30890.22832062750106807No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC28020.2071072833467118No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC27890.20614640016201968No Hit
GTCTTTGGCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACATCTACTTAGCCTGGTA27430.2027463519700323No Hit
GTCTCCAGCCACCCTGTCTTTGGCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAT27030.19978978832482583No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT26500.19587234149492727No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25980.19202880875615888No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTG24670.18234606281810778No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24360.1800547259930728No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24010.17746773280351713No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACATCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGTCTCCCAGGCT23760.1756198805252631No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23480.17355028597361857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22460.16601104867834213No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA22300.16482842322025956No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21590.1595805227500181No Hit
GTTTACTACTGTCAGCAGCGTAAATACTGGCCTCCGCTCATTTTCGGCGG21500.15891529592984666No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21450.15854572547419588No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21230.1569196154693323No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATA20800.15374130955073537No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA20450.15115431636117974No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGCGTAAATACTGGCCTCCGCTCATTTTCGGCGGAG20130.14878906544501458No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT20030.14804992453371296No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAATGAGCGGAGGCCA20020.1479760104425828No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19800.14634990043771925No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19560.14457596225059538No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGGCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCA19220.1420628831521699No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA19010.14051068723843652No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC18800.13895849132470314No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAATGAGCGGAGGCCAGTATTTACGCT18660.13792369404888086No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGCGTAAATACTG18190.1344497317657633No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18070.13356276267220135No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17640.1303844567536044No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17330.12809311992856942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA16590.12262347718493749No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTT15710.1161190371654833No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15710.1161190371654833No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15540.11486249761627056No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGAC15410.11390161443157847No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15370.11360595806705782No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGGCTCCA15200.11234941851784508No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14910.11020590987507041No Hit
GGCCAGTATTTACGCTGCTGACAGTAGTAAACTGCAAAATCTTCAGGCTC14740.10894937032585765No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14350.10606672077178138No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14300.10569715031613057No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14140.10451452485804799No Hit
GCACTGAACACAGAGGACTCACCATGGACTTGGGGCTGTGCTGGGTTTTC14040.10377538394674639No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTG13950.10311015712657493No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13820.10214927394188282No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGCGGGCGTAGCGGTTGCTGGTACCCTCTT13770.10177970348623203No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13750.10163187530397169No Hit
CAGTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGGTCATTTGGAGATAGAGTGAGTTCTTGGCATTGTCTCTG13680.10111447666606058No Hit
ACAGAAACCTGGCCAGTCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT13620.1006709921192796No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGAA13570.10030142166362879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3150.068.84431570
GTTATTA900.007419611351.6332470
GGTAAGC200.00746350351.56626544
CGTTGTC200.00798230950.697117
CAGTTAA950.00918728248.915770
CTTCCAT5050.046.00981570
TTACTTT1754.2848667E-544.2570670
TAGGCAA1805.059949E-543.027770
GGTATCA10550.039.764431
GCAGCGT31950.039.7551770
GAGCAAA24650.038.96057570
GATTCGA350.001486053538.65351517
CCTGCTT8950.038.0759170
AAGGACA25700.036.7660870
GACAGGT3050.036.6049351
GTATCAA24600.036.582391
TCCCGCA8050.036.5601870
CGGCAAA1700.001980890336.44699570
AAACGAA23700.034.64002270
TTTAAGT1900.003417652332.61046670
Produced by FastQC (version 0.11.5)