FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749243
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA46180.6163554414255455No Hit
CTCCTAAACTGCTCATTTATTGGGCATTTACCCGGGAACCCGGGGTCCCTGCCCGATTCAGTGGCAGCGGGTC31840.4249622619096875No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31590.42162556073263285No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGGTGGAGAAAAGAT30170.40267309804696205No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT28960.3865234643500173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28440.3795831259017435No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT19410.25906147938652746No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18000.24024248474793894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17240.2300989131696926No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17120.22849729660470636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16580.2212900220622682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16350.21822025697937783No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15480.20660853688322747No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15390.20540732445948778No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15060.2010028789057756No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14960.1996681984349537No Hit
ATACTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA14830.19793311382288523No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14310.19099277537461143No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAA13970.18645486177381704No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13540.18071573574928293No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13530.18058226770220076No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAG12660.16897054760605038No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11930.15922738016905064No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTTTCTCCACCTCCAG10800.14414549084876335No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10740.14334468256627023No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10700.14281081037794147No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC10550.14080878967170862No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10430.13920717310672237No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10280.13720515240048956No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9990.1333345790351061No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.13079868614054452No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTAC9780.13053175004638015No Hit
GTAATACATGGCCGTGTCCGCGACAGTCACAGAGTTCAACCTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA9680.12919706957555827No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9510.12692811277516106No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTTTCTC9490.1266611766809967No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9250.12345794355102417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9150.12212326308020229No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8890.1186530938560654No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8780.11718494533816133No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8650.11544986072609287No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8550.11411518025527098No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG8450.11278049978444911No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8140.10864299032490128No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTATTGGGCATTTACCCGGGAACCCGGGGTCCCTGCCCGATTCAG8100.10810911813657251No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8090.10797565008949034No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATTTATTGGGCATTTACCCGGGAACCCGGGGTCCCTGC7990.10664096961866844No Hit
GAACTACTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGC7940.1059736293832575No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7920.10570669328909313No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTTTCTCCACCTCCAGCAATAAGAACTA7900.10543975719492875No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7810.10423854477118906No Hit
CTACTTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATTTATTGGGCATTTACCCGGGAACC7810.10423854477118906No Hit
GTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGGTG7720.10303733234744936No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG7650.10210305601787405No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7640.10196958797079184No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7510.10023450335872341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA6900.051.01113570
TCGTATG3050.041.6266540
GGTATCA7800.040.8778231
CTCGTAT2800.040.3895239
CGTATGC3250.039.11255341
CTTATAC4000.039.0078851
ACGAGAC3600.038.65163822
GCGTAGA18300.038.46741570
GTATCAA17150.037.776681
TATACAC4100.037.2241863
ATCTCGT2400.037.06323237
GAGCGAT3000.036.34132832
TTATACA4400.035.4569782
CGAGACA3950.035.23386823
ATCTCCG3950.035.20094710
TATTCTA2008.2907536E-735.19768570
CAGTTCG13150.035.17856670
ATGCCGT3650.034.90175644
CACGAGA3900.034.80590421
TCTCCGA4000.034.76093711
Produced by FastQC (version 0.11.5)