FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775259
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35670.46010430062727425No Hit
ATATAGTACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA34970.4510750600766969No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT22300.28764580611124796No Hit
GAAATAGACAGACGTGTCCTCGGCTGTCAGGCTGTTCATCTGCAGATACA21180.2731990212303243No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20600.26571765048841745No Hit
GGTAAGTACTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAA16520.2130900769936241No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCCATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAAGGG15540.20044914022281585No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15390.1985143029619779No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT15380.19838531381125535No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA15330.19774036805764267No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15310.1974823897561976No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14320.18471246383466686No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14230.18355156147816407No Hit
GTCTATTTCTGTGCGAGGACTCAGCTAAATACAGCCACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACGG14110.18200369166949368No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13540.1746513100783093No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13490.17400636432469666No Hit
GTACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA13410.17297445111891638No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC13140.169491744049408No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12950.16704095018567988No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12910.16652499358278974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12830.1654930803770095No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12690.16368723226689402No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC12170.15697979642932233No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11920.15375506766125902No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11820.15246517615403368No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11730.1513042737975309No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC11570.14924044738597037No Hit
GTATTTAGCTGAGTCCTCGCACAGAAATAGACAGACGTGTCCTCGGCTGTCAGGCTGTTCATCTGCAGATACAG11470.14795055587874503No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11420.14730561012513238No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGG11390.14691864267296478No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATCATTTAGCCTGGTTTCAGCAGAAA11100.14317795730201133No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA11080.14291997900056627No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11080.14291997900056627No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATGGCTAC11040.14240402239767613No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10960.14137210919189588No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG10920.14085615258900575No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT10740.13853434787600016No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGCGAAATACTA10600.1367284997658847No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10530.13582557571082696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10140.1307949988326482No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10050.12963409647614538No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10040.12950510732542286No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10010.12911813987325527No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9940.12821521581819753No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG9940.12821521581819753No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTT9910.12782824836602993No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAA9880.12744128091386234No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9750.1257644219544694No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC9740.1256354328037469No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAGATGAACAGCCTGACAGCCGAGGACACGTCTGTCTATT9510.12266868233712862No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGGTGGCTGTATTTAGCTGAGTCCTCGCACAGAAATAGACAGACGTGTCC9450.12189474743279344No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGA9440.12176575828207091No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG9430.12163676913134835No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9420.12150777998062583No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATCATTTAGCCTGGTT9360.12073384507629062No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACA9340.12047586677484556No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9270.11957294271978784No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG9200.1186700186647301No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACCGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGGTGGCTGTAT9110.11750911630822733No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9100.11738012715750477No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT9040.1166061922531696No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8990.11596124649955691No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATG8920.11505832244449918No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC8860.114284387540164No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG8820.11376843093727387No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8560.11041471301848803No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8410.10847987575765003No Hit
GCTAAATACAGCCACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACGGTCTCCTCAGCATCCCCGACCAGCCC8390.10822189745620496No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8390.10822189745620496No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8290.10693200594897963No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA8250.10641604934608949No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAG8190.10564211444175431No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACGATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAGATGAA8150.10512615783886418No Hit
ACATAAAGCAAGATGGAAGTGCGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACGATCTCCAGAGACAACG8150.10512615783886418No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8090.10435222293452898No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGG8060.10396525548236138No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7960.10267536397513605No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7900.10190142907080087No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC7900.10190142907080087No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7880.10164345076935578No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT7850.10125648331718819No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7840.10112749416646566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAG100.0024832026205.893870
CCGCTAG150.008350373137.2625470
GTGTATT150.002525098967.7988361
TCGCACG701.8717446E-458.826870
ACTTATA200.00617682254.09110665
TGTTAGA5650.052.84000870
GTGGTAT1400.050.849131
CTCTTAT705.2568794E-1043.584971
TCTGGAG11950.042.21253670
TGGTATC1700.041.8649522
GGTATCA5300.040.9351461
GACAGGT1250.040.6793021
CGCCCGA6100.040.503770
ATCTCGT1550.039.65875237
CGTATGC1650.039.47115341
TCGTATG1700.038.2628140
GTATCAA12950.038.2186471
TGCACAG6850.037.57186570
AATCTCG1650.037.22857736
ATGCCGT1900.036.15950444
Produced by FastQC (version 0.11.5)