FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775259
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34030.4389500799087788No Hit
ATATAGTACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32000.4127652823121047No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20940.2701032816129835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA20580.26545967218697236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20540.2649437155840822No Hit
GAAATAGACAGACGTGTCCTCGGCTGTCAGGCTGTTCATCTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA17870.23050361234116598No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCCATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAAGG15940.20560870625171718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15160.1955475524953596No Hit
GGTAAGTACTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT14720.18987202986356816No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14130.18226166997093873No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC13890.17916593035359796No Hit
GTCTATTTCTGTGCGAGGACTCAGCTAAATACAGCCACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACGGT13550.17478029922903185No Hit
GTACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA13260.1710396138580784No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA13240.17078163555663334No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12940.16691196103495737No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCA12890.1662670152813447No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12360.15943059029305046No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12280.1583986770872702No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12190.1572377747307674No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA12010.1549159700177618No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATGGCTACTAAAGGTGAATCCAGAGGCTGCACA11850.15285214360620128No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11570.14924044738597037No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11550.1489824690845253No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11360.1465316752207972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC11310.1458867294671845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11290.14562875116573945No Hit
GTATTTAGCTGAGTCCTCGCACAGAAATAGACAGACGTGTCCTCGGCTGTCAGGCTGTTCATCTGCAGATACAGT11150.14382290305562398No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11000.141888065794786No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG10930.14098514173972826No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGCGAAATACTA10920.14085615258900575No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10810.13943727193105787No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10640.13724445636877483No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGG10500.13543860825865936No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT10300.13285882524420872No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATGATTGCTAATGCCCTGACTC10200.1315689337369834No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAGATGAACAGCCTGACAGCCGAGGACACGTCTGTCTATTT10110.1304080313804806No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC10040.12950510732542286No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10010.12911813987325527No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA9980.12873117242108767No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9810.1265383568588046No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9650.12447453044724408No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC9640.12434554129652155No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGGTGGCTGTATTTAGCTGAGTCCTCGCACAGAAATAGACAGACGTGTCCT9600.12382958469363142No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATGATTGCTAATGCCCTGACTCGCCC9550.12318463894001877No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATCATTTAGCCTGGTTTCAGCAGAAA9480.12228171488496103No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9470.12215272573423848No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9420.12150777998062583No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9420.12150777998062583No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT9240.11918597526762023No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACCGTGACCAGGGTTCCCTGG9120.11763810545894984No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG9080.11712214885605972No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGA9010.116219224801002No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACA8960.11557427904738933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8910.11492933329377665No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8840.11402640923871893No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGT8800.11351045263582879No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG8770.1131234851836612No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC8530.11002774556632042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8250.10641604934608949No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC8250.10641604934608949No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8250.10641604934608949No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT8220.10602908189392189No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC8190.10564211444175431No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8140.10499716868814163No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT8050.10383626633163885No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7930.10228839652296846No Hit
GCTAAATACAGCCACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACGGTCTCCTCAGCATCCCCGACCAGCCC7910.1020304182215234No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG7910.1020304182215234No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATA7870.10151446161863326No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCATGGTCCAGCCTGGGGGGTCCCTCAGACTCTCCTGTGCAGC7820.1008695158650206No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT7780.10035355926213045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA1900.083.1086470
GCCTTAC150.0025527267.61304516
TAAGTCA1001.3154143E-463.1625770
AACTGTT900.00687122452.63547570
GGTATCA5200.044.867341
GAGCAAA12300.041.08134570
AGACACA10700.039.84554370
GTGGTAT1550.039.266791
GAGCCAT11050.038.58346670
GTATCAA13150.038.0557751
CTTCTAA5401.8189894E-1235.09031370
TGGTATC1650.034.8174862
CGATTCA10250.033.89211370
AAGCGGT8000.033.55511570
AGTGCTA2851.7218588E-533.24345870
AAGGACA13300.033.24345870
AAGGAAA5355.638867E-1132.4667470
GAGTTTA3350.032.299051
CTGTATT10350.032.03898270
CCGCATT500.00854944131.58128570
Produced by FastQC (version 0.11.5)