FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169669
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47140.4030199996751218No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT46310.3959239750732899No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33240.2841829611625169No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31980.2734106828513024No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT29760.2544309543982101No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29140.24913030951491405No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28100.2402389051945465No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG26970.23057805242337792No Hit
CCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG26160.22365301636616852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25720.2198912683844746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25710.21980577411216334No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25640.21920731420598474No Hit
ATTTAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA24800.2120257953318417No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24570.21005942706868352No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23980.2050152650023212No Hit
GTCTCCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG23520.20108252847600475No Hit
CTATTAAATTGTTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC22560.192875078334127No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22530.19261859551719332No Hit
GTGCGGTCCCATCAAAATTCAGCGGCAGTGGATCTGGGACAGAATTCACT22230.19005376734785653No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22190.18971179025861162No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22120.18911333035243305No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22040.18842937617394323No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21350.18253027138446862No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21280.18193181147829002No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20960.17919599476433076No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACA20210.17278392434098877No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20200.17269843006867755No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19210.16423449710986612No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19210.16423449710986612No Hit
GTCCAAGGGTAACTATTAAATTGTTGACAGTAATAAGTTGCAAAATCCTC19000.16243911739133038No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18950.16201164602977425No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18870.16132769185128443No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18280.15628352978492205No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17750.15175233335242705No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG17270.14764860828148818No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17160.1467081712860647No Hit
GGGCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCC16960.14499828583984017No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA16850.14405784884441666No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA16510.14115104358583497No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16480.14089456076890128No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16250.1389281925057431No Hit
CACCAGGGAAAGCCCCTAACCTCCTGATCTTTGGTGCATCGACTTTGCAA16070.13738929560414098No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15690.1341405132563144No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG15190.12986579964075307No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14980.12807041992221732No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14870.1271299829267938No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14750.1261040516590591No Hit
ATCTATTACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAAGGTCGAGT14600.1248216375743907No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC14560.1244796604851458No Hit
GCTAATGCCCTGACTGGCCCGGCAAGTGATGGTGAT14550.12439416621283458No Hit
CAATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA14190.12131637240963042No Hit
GGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGCCCTGACTGGCCCGGCAAGTGA14180.12123087813731918No Hit
GTACAACCCCTCCCTCAAAGGTCGAGTCACCATGTCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAC14130.12080340677576307No Hit
GTAATAGATATAGCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGCTCCAGTAGTAATTACTGA14120.12071791250345185No Hit
GTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14040.12003395832496201No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13950.11926450987416097No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAACAATTTAATAGTTAC13900.11883703851260485No Hit
ACTTATTACTGTCAACAATTTAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13900.11883703851260485No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13880.1186660499679824No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13360.1142203478077986No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13200.11285243945081899No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13160.1125104623615741No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13160.1125104623615741No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13120.11216848527232919No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13090.1119120024553955No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13090.1119120024553955No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG13030.11139903682152814No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13010.11122804827690569No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13010.11122804827690569No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12990.11105705973228325No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12930.11054409409841587No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12790.1093471742860587No Hit
GGACCGCACTTTGCAAAGTCGATGCACCAAAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGG12780.10926168001374748No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12670.10832124301832398No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12640.1080647602013903No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12530.1071243232059668No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12490.10678234611672191No Hit
TTGCAAAGTCGATGCACCAAAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAA12300.1051579549428086No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12210.10438850649200757No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12160.10396103513045143No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12110.10353356376889529No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12050.10302059813502794No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12030.1028496095904055No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGATTCCTGCAGCTGCAC11970.10233664395653812No Hit
ACTTTGCAAAGTGCGGTCCCATCAAAATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGC11930.10199466686729322No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11830.10113972414418095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.051.7420271
TATAGTT200.00820214750.349425
CGTATGC2250.050.3046441
TCGTATG2250.050.18152240
ATCTCGT2300.047.334137
TGTTAGA12950.046.4961270
ACGAGAC2450.045.1652322
CTTATAC2500.044.193781
TATACAC2500.044.1767773
AATCTCG2400.043.87373736
ATGCCGT2600.043.8247144
AATGGTA2505.0931703E-1141.52584570
GCGTAGA28800.041.09328570
CCCACGA2900.040.4416519
GTATGCC2900.040.30576342
CACGAGA2850.040.00108721
CGAGCCC2850.039.9463415
GTATCAA21850.039.5326081
CGAGACA2800.039.5280423
TCCGAGC2800.039.44523613
Produced by FastQC (version 0.11.5)