FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699155
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGTTAACGCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG73010.4296841665416045No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT50510.2972654054515333No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGATCTGGCTTTTT45810.26960459757938504No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44790.2636016137433018No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA43290.2547736963372971No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT39660.23341013621476558No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA39200.23070290821025746No Hit
CTCCTGTGCAGCCTCGGGAATCACCTTTTCCAACTATGCCATGAACTGGG38720.2278779746403359No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT36860.21693135705689004No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC33780.19880469998322697No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG33040.19444959406293127No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC32970.19403762458398438No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC31280.18409150430655238No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31110.18309100700053849No Hit
GTGTATTACTGTCAGCAGTATGTTAACGCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA30700.18067804290956388No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30370.17873590108024281No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA30320.17844163716670935No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG29760.17514588133513423No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA29650.17449850072536055No Hit
GAAATATGTGGCCGTGTCTTCGACTCTCAGGCTGCTCATTTGCAGATACAGGGTGCTCTTGGAATTGTCTC29500.1736157089847601No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA28780.1693783086298778No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27770.16343417757650128No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG27380.16113891905094002No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG27050.15919677722161898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26760.15749004652312473No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA25530.15025115425020083No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25100.14772048459381282No Hit
GAATACGGCTTTGGCTACGGGGGAGGTCTCTTTGACGCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCA24590.1447189926757712No Hit
GGGTTCCCTGGCCCCAGGCGTCAAAGAGACCTCCCCCGTAGCCAAAGCCGTATTCAGGTCCTGGGGAGAATTT24590.1447189926757712No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGCCAGCAACTGCTTAGCGTGGTACC24510.1442481704141176No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24450.14389505371787742No Hit
GAACAGAGAGAACTCACCATGGAATTTGGGCTGATCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG24300.14301226197727693No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24270.14283570362915685No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC24260.14277685084645014No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC24110.14189405910584968No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24080.14171750075772957No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT24050.1415409424096095No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT23980.1411289729306626No Hit
CCCTGAGACTCTCCTGTGCAGCCTCGGGAATCACCTTTTCCAACTATGCC23940.1408935617998358No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23890.14059929788630232No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG23840.1403050339727688No Hit
TAACACATACTACGCAGACTCGGTAAAGGGCCGGTTCACCATTTCCAGAGACAATTCCAAGAGCACCCTGTA23440.13795092266450087No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG23000.13536140022540616No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22900.13477287239833918No Hit
GTATGTGTTATGACCACGACCACTAATGGTTGAGACCCACTCTGGGCCCTTTCCTGGAGCCTGGCGGACCCAGT22880.13465516683292578No Hit
CCCTGTATCTGCAAATGAGCAGCCTGAGAGTCGAAGACACGGCCACATATTTCTGTGCAAAATTCTCCCCAGGAC22800.13418434457127218No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGTCAAAGAGACCTCCCC22620.13312499448255163No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG22610.1330661416998449No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGATCTGGCTTTTTCTTGTGGCTATTTTAAAAGG22240.13088858873969708No Hit
GAGTGGGTCTCAACCATTAGTGGTCGTGGTCATAACACATACTACGCAGACTCGGTAAAGGGCCGGTTCACCATT22170.1304766192607502No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC22090.13000579699909662No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21820.12841677186601574No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT21810.12835791908330907No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGTGGACGCACCGAAGATGAGGAGCC21790.12824021351789566No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG21440.12618036612316122No Hit
GCCTGAGAGTCGAAGACACGGCCACATATTTCTGTGCAAAATTCTCCCCA21250.12506216325173394No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAG21230.12494445768632056No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21170.12459134099008036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21020.12370854924947988No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20930.12317887420511961No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC20710.12188411298557224No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20670.12164870185474544No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT20670.12164870185474544No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20340.1197065600254244No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGAAT20250.1191768849810641No Hit
GTCATAACACATACTACGCAGACTCGGTAAAGGGCCGGTTCACCATTTCC19110.11246766775250051No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAG19000.11182028714272682No Hit
GCCGTATTCAGGTCCTGGGGAGAATTTTGCACAGAAATATGTGGCCGTGTCTTCGACTCTCAGGCTGCTCATT18820.11076093705400625No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18530.10905420635551201No Hit
GTAAAGGGCCGGTTCACCATTTCCAGAGACAATTCCAAGAGCACCCTGTA18170.10693550617807088No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA18010.1059938616547637No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17760.10452254208709623No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG17460.10275695860589527No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG17300.10181531408258811No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG17240.10146219738634793No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC17220.10134449182093452No Hit
GTCTTTGTCTCCAGGTGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAGCAACTGCTTAGCG17160.10099137512469435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)