FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699155
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGTTAACGCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT66450.39107674108601037No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48610.2860833767372606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA45580.268250983577131No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGATCTGGCTTTTT45140.26566146113803624No Hit
CTCCTGTGCAGCCTCGGGAATCACCTTTTCCAACTATGCCATGAACTGGG39030.22970241090424356No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC37590.22122761019447904No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA37160.21869694053809097No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC36430.21440068740050203No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG34210.20133536963961499No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34000.2000994612027743No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32810.1930959800606772No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT30720.18079574847497726No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG30440.1791478705591897No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA30330.17850048994941603No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC30290.17826507881858925No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29850.17567555637949453No Hit
GTGTATTACTGTCAGCAGTATGTTAACGCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA29720.17491047020430744No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA29210.1719089782862658No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG28560.16808354741033044No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG28390.16708305010431657No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC27470.16166859409530032No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG26850.158019721567485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25130.1478970429419329No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT25020.14724966233215922No Hit
GGGTTCCCTGGCCCCAGGCGTCAAAGAGACCTCCCCCGTAGCCAAAGCCGTATTCAGGTCCTGGGGAGAATTTT24860.14630801780885205No Hit
GAAATATGTGGCCGTGTCTTCGACTCTCAGGCTGCTCATTTGCAGATACA24690.14530752050283818No Hit
GAACAGAGAGAACTCACCATGGAATTTGGGCTGATCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT23900.14065815066900902No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23710.13953994779758175No Hit
GAATACGGCTTTGGCTACGGGGGAGGTCTCTTTGACGCCTGGGGCCAGGG23570.13871600883968796No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG23060.13571451692164635No Hit
GAGTGGGTCTCAACCATTAGTGGTCGTGGTCATAACACATACTACGCAGA22780.1340666390058588No Hit
TAACACATACTACGCAGACTCGGTAAAGGGCCGGTTCACCATTTCCAGAGACAATTCCAAGAGCACCCTGTAT22630.1331838472652583No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22580.13288958335172485No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGTCAAAGAGACCTCCCC22480.13230105552465785No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22360.13159482213217746No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA22340.13147711656676406No Hit
CCCTGTATCTGCAAATGAGCAGCCTGAGAGTCGAAGACACGGCCACATATTTCTGTGCAAAATTCTCCCCAGGAC22300.13124170543593727No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGCCAGCAACTGCTTAGCGTGGTACCAACAAAAACCTGGCCAGGCTCCCAG22180.1305354720434569No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT22130.1302412081299234No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT21970.12929956360661624No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC21930.12906415247578945No Hit
GCCTGAGAGTCGAAGACACGGCCACATATTTCTGTGCAAAATTCTCCCCAGGACCTGAATACGGCTTTGGCTACG21700.1277105384735354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21460.12629807168857463No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21360.12570954386150762No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGAATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACA21190.12470904655549377No Hit
GTATGTGTTATGACCACGACCACTAATGGTTGAGACCCACTCTGGGCCCTTTCCTGGAGCCTGGCGGACCCAGTT21050.12388510759759998No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGATCTGGC21010.12364969646677319No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT21000.12359084368406649No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG20980.12347313811865308No Hit
CCCTGAGACTCTCCTGTGCAGCCTCGGGAATCACCTTTTCCAACTATGCCATGAACTGGGTCCGCCAGGCTCCAG20860.12276690472617272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20830.1225903463780526No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGTGGACGCACCGAAGATGAGGAGCCTG20710.12188411298557224No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT20610.12129558515850526No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGAAT20600.12123673237579854No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC20540.12088361567955837No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20450.1203539406351981No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC20130.11847065158858373No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC20080.11817638767505025No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19930.11729359593444977No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT19830.11670506810738279No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCA19810.11658736254196939No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA19670.1157634235840756No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTG19260.11335045949310099No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG19250.11329160671039429No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19090.11234996218708711No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT19090.11234996218708711No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC18930.11140831766377993No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG18680.10993699809611249No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC18610.10952502861716559No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18550.10917191192092539No Hit
GTGTGAGGTGCAGTTGATGGAGTCTGGGGGAAACTTGGTAGAGCCTGGGGCGTCCCTGAGACTCTCCTGTGCAGC18250.10740632843972446No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTC17520.10311007530213548No Hit
GTCTTTGTCTCCAGGTGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAGCAACTGCTTAGCGTG17320.10193301964800151No Hit
GCCGTATTCAGGTCCTGGGGAGAATTTTGCACAGAAATATGTGGCCGTGTCTTCGACTCTCAGGCTGCTCATTT17230.10140334460364121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCCA25250.059.52549770
ACGTAAT300.00646881753.4648969
GAGCCAT25300.047.7592670
GAGCAAA23900.046.8575970
GTGGTAT3900.046.1447031
TGGTATC4100.044.685072
TCAGCAT24650.044.23632470
TATTCTA1755.756638E-542.1013770
GGTATCA11350.041.5843121
GTATCAA30700.041.0342141
AATGGTA3301.1623342E-940.18766870
TGCGTTA14250.037.2264770
GAACTGT24450.035.64325769
TATCAAC36150.034.6314432
ATATAGC502.0551714E-433.924566
TATGGTT1750.002918499533.68109570
ATCAACG37100.033.5558323
TCAACGC37400.033.1062434
CAACGCA37450.033.0630155
AACGCAG37850.032.8041956
Produced by FastQC (version 0.11.5)