FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373681
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCCCGTACCTGCCTGCATATGAGGAGCAGTAATAATCAGC47080.34272877036226024No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG44510.3240199143760451No Hit
GATTATTACTGCTCCTCATATGCAGGCAGGTACGGGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCGG43320.31535705888048243No Hit
GTCCTAGGTCGGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42190.30713098601494815No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGATTATAACTA40370.2938819129040876No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38920.283326332678402No Hit
GCCTTGGGCCGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCCCGTACCTGCCTGCATATGAGGAGCAG38440.2798320716381751No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG37990.2765562019129623No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG37360.27196998429766445No Hit
GTAATATAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA33880.24663659175601904No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGA32230.2346250694302389No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT29850.2172993584391136No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCCGACCTAGGACGGTCACCTTGGTCCCTC29480.21460586555393865No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC27670.20142958954808285No Hit
GTAATAAGAGAGACCCACTCCAGACCCTTCCCCGGAGCTTGACGGACCCA27280.19859050245289844No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG26530.19313071957754382No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25670.1868701685471372No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT25330.18439506697697647No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25260.18388548724194337No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCC25050.18235674803684407No Hit
GTGTTGGTACCAGGAGACATAGTTATAATCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA24850.1809008059367495No Hit
CTCTTATTACTTGGGATGGTGGTGGCACATACTATGCAGACTCTGTGAAG24580.17893528410162185No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAG24540.17864409568160294No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGATTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGC24330.17711535647650364No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24110.17551382016639963No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTCCTCATAT23610.17187396491616322No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23550.17143718228613483No Hit
GTATGTGCCACCACCATCCCAAGTAATAAGAGAGACCCACTCCAGACCCTTCCCCGGAGCTTGACGGACCCAG22850.16634138493580386No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22630.16473984862569985No Hit
GTCTGAGAACTGAGGACACCGCCTTATATTACTGTGCAAAAGATTCGAAT22480.16364789205062893No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22170.16139118179548237No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT21890.15935286285534997No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATAGTATGCACTGGGTCCGTCAAGCTCCGGGGAAGGG21550.1568777612851892No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21500.15651377576016556No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21450.15614979023514192No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21000.15287392050992915No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC20510.14930686236469748No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20450.14887007973466912No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC20430.14872448552465967No Hit
TTATATTACTGTGCAAAAGATTCGAATAGTAATCACGCCACATTTCAATTTGACTACTGGGGCCAGGGAACCC20350.14814210868462183No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19810.1442110650143665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19750.14377428238433815No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG19630.1429007171242814No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC19610.14275512291427195No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGATTATAACTATGTCTCCTGGTACCAACACCA19260.14020722423910645No Hit
CTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTGCATACTATAATCAT18860.13729534003891733No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18750.13649457188386532No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATCTGCAAATGAA18680.13598499214883222No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18660.13583939793882277No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18260.13292751373863365No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18000.1310347890085107No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17990.130961991903506No Hit
GGTCTGGAGTGGGTCTCTCTTATTACTTGGGATGGTGGTGGCACATACTA17880.13016122374845399No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG17560.12783171638830268No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT17500.1273949337582743No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT17290.125866194553175No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17280.12579339744817028No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA17280.12579339744817028No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17260.12564780323816083No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG17220.12535661481814192No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAG17220.12535661481814192No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGG17010.12382787561304262No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGA16990.12368228140303317No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT16950.12339109298301425No Hit
GGCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATC16920.12317270166800007No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16860.1227359190379717No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGT16820.1224447306179528No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16760.12200794798792441No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16560.12055200588782986No Hit
GGATGAGGCTGATTATTACTGCTCCTCATATGCAGGCAGGTACGGGGTATTCGGCGGAGGGACCAAGGTGACCG16450.11975123773277783No Hit
CCTTGGTCCCTCCGCCGAATACCCCGTACCTGCCTGCATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGC16440.11967844062777311No Hit
GGTACGGGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCGGCCCAAGGCTGCCCCCTCGGTCACTCTGT16130.11742173037262654No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG15810.11509222301247524No Hit
GTCTGCATAGTATGTGCCACCACCATCCCAAGTAATAAGAGAGACCCACT15760.1147282374874516No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15650.11392746933239958No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG15540.11312670117734759No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15540.11312670117734759No Hit
GCATAGTATGTGCCACCACCATCCCAAGTAATAAGAGAGACCCACTCCAGACCCTTCCCCGGAGCTTGACGGACC15330.1115979619722483No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG15050.1095596430321159No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATTGAAATG14950.10883167198206861No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14880.10832209224703553No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14800.1077397154069977No Hit
GAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTG14740.10730293277696933No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG14680.10686615014694097No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTCTTTCCATTCGGTGA14600.10628377330690314No Hit
GGGTTCCCTGGCCCCAGTAGTCAAATTGAAATGTGGCGTGATTACTATTCGAATCTTTTGCACAGTAATATAAGG14590.1062109762018984No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTCT14450.10519181673183221No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14410.1049006283118133No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14400.10482783120680857No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGAC14360.10453664278678966No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG14330.10431825147177547No Hit
GAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAGTCTGGGGGAGTCG14320.10424545436677074No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC14310.10417265726176601No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCCGACCTAGGACGGTCACC14270.1038814688417471No Hit
ATATTACTGTGCAAAAGATTCGAATAGTAATCACGCCACATTTCAATTTGACTACTGGGGCCAGGGAACCCTGG14230.1035902804217282No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14210.10344468621171873No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14180.10322629489670455No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT14000.10191594700661945No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13990.10184314990161472No Hit
GATTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCACTAAG13950.1015519614815958No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCCGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCCCGTACCTGC13870.10096958464155797No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG13870.10096958464155797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA150.0077399947140.8042470
AGGGGGG3400.071.43745470
GTGGTAT2000.047.5967861
TTAACGG451.9993313E-645.5258535
GGTATCA7950.041.9091221
TCGTATG2950.041.83138340
TGGTATC2400.041.0730822
CGTATGC3100.039.83945541
TACGGAG603.270743E-739.656772
GTATCAA20550.038.878161
CTTATAC3250.038.7050821
TATACAC3350.038.5575453
GCCGTCT3250.038.1436446
AATGGTA3059.094947E-1238.086470
CTATTAC451.0920057E-437.7752271
ACGAGAC3300.037.08570522
TACACAT3300.037.0830045
TCTCCGA3300.037.08165711
CGAGCCC3400.036.9921215
ATACACA3400.036.992124
Produced by FastQC (version 0.11.5)