FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210755
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGTGGGGTCCCATCAAGGTTCAGTGGCGGTGGATTTGGGACTGATT48090.397190182984997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38200.31550561426547896No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT37990.31377115931794625No Hit
GAGTTACAGTCCCCCTCGAACTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCA36410.3007214506650809No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT27020.22316653658254562No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26500.21887169576008358No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26220.21655908916337327No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG26190.21631130988515432No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24510.2024356703048924No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23460.1937633955672287No Hit
CCACTGTACAGCCTGGATGCAGCATAGATCACGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAA23270.19219412680517528No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22890.18905558928106841No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22850.1887252169101098No Hit
GTATAGGGTATGCAGATTCTGTGAAGGGTCGATTTATCATCTCCAGAGAC22810.1883948445391512No Hit
CTGTAACTCTGGTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAAATTGCTGATGGTGAGAGTGAAATCAGTC21780.17988775598696682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21210.17517994970080653No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21100.17427142568067033No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20800.1717936328984807No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT20780.1716284467130014No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19610.16196505486246185No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19610.16196505486246185No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19520.16122171702780497No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18780.15510982816507055No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18640.1539535248667154No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18230.15056720806438958No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17300.14288605043960173No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17240.1423904918831638No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16250.13421377570193804No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16180.13363562405276047No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16120.13314006549632254No Hit
GTCTGAGAGTCGAGGACACGGCCTTGTTTTATTGTGCGAGAGAGAGTGAGGCTTACGATTTGTGGAGTGGCCACG15850.1309100519923519No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15820.13066227271413292No Hit
GATCAGCACTGAGCACAGAGGACTCGCCATGGAGTTTGGGCTGACCTGGGTTTTCCTTCTTACAATTTTAAAAGG15650.1292581901375588No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15540.1283496661174226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15400.12719336281906746No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCCTGGCCAAAAGTTCGAGGGGGA15340.1266978042626295No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG15300.1263674318916709No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15260.12603705952071229No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15100.12471557003687782No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGACCATTACCAA15020.12405482529496059No Hit
GATTTCACTCTCACCATCAGCAATTTGCAACCTGAGGATTTTGCAACTTACTACTGTCACCAGAGTTACAGTCCC14900.12306370818208473No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA14800.12223777725468819No Hit
GTACAGCCTGGATGCAGCATAGATCACGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGT14540.12009035684345719No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14390.11885146045236238No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14330.11835590189592443No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14260.11777775024674687No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14070.11620848148469344No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13780.11381328179524346No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCCTGGCCAAAAGTT13700.11315253705332623No Hit
GTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGTTGGTAATGGTCTGACTTGCCCGGCAAGTGA13670.11290475777510726No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13650.11273957158962795No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13420.11083993045661591No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13160.10869251004538491No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG13120.10836213767442629No Hit
CAATAAAACAAGGCCGTGTCCTCGACTCTCAGACTGTTCAGTTGCAGATACAGGGAGTTCTTGGCGTTGTCTC13110.10827954458168663No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG13040.10770139293250905No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC12910.10662768272689356No Hit
ACTTACTACTGTCACCAGAGTTACAGTCCCCCTCGAACTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAA12880.1063799034486746No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG12750.10530619324305908No Hit
GTTTTATTGTGCGAGAGAGAGTGAGGCTTACGATTTGTGGAGTGGCCACG12730.10514100705757978No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12660.1045628554084022No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12450.10282840046086944No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12330.1018372833479936No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCGCTGGTATTAATTGGAATGGTGGC12290.10150691097703499No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAAATTGCTGATGGTGAGAGTGAAATCAGTCCCAAATCCACCGCCA12270.10134172479155568No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12240.10109394551333671No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12190.10068098004963845No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12140.10026801458594017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG150.002535655267.729865
CGTATGG451.9364525E-645.73691641
TGTTAGA11850.044.54491870
TATGGCG704.5838533E-1044.2014643
CTACGCT4700.041.87030828
ACTACGC4750.041.4107227
TATACAC4850.040.4965933
GTATCAA23150.040.239951
ACGAGAC5000.039.9738222
CGAGACT5050.039.58785623
TACGCTG5000.039.36786329
CACGAGA5150.038.80632821
TTATACA5000.038.604422
TCGTATG2400.038.5502140
CTTATAC5150.038.150171
TGCATCG1450.037.56678834
GTGGTAT2100.037.1008831
GAGACTA5400.037.0250424
ATCGCGT655.906877E-736.77520437
AAAGGGG4300.036.6199768
Produced by FastQC (version 0.11.5)