FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969146
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT57960.598052305844527No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG36790.3796125661149094No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30740.3171864713882119No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGAACCACTCAGG30490.314606880697026No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG25410.2621895978521296No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC24180.2494980116514952No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGAACCACTCAGGCTGCTGTCGTAGGA23750.24506111566265557No Hit
GATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGG22050.2275198989625918No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG21810.22504349189905337No Hit
GGCTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21760.22452757376081622No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG21640.22328937022904702No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT21070.21740790345314331No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20880.21544741452784202No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19260.19873166684895774No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18220.1880005695736246No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17890.1845955098612593No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17710.18273820456360548No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17570.18129363377654142No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17030.17572171788358No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC16250.1676733949270801No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC16210.1672606604164904No Hit
GTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG16110.16622882414001605No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16030.16540335511883658No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15860.1636492334488302No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGGCGGAGGGACCAAG15730.16230784628941355No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15670.16168874452352897No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15620.1611728263852918No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTC15600.16096645912999694No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGGGCAGCTCCAACATCGGGGCAGGTTATCA15330.1581805011835162No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGG15220.15704548127939444No Hit
GGGCAGAGGGTCACCATCTCCTGCGCTGGGGGCAGCTCCAACATCGGGGCAGGTTATCATGTACACTGGTATCAG15210.156942297651747No Hit
GTGCATATAGTGGTCGATGAAGGTGAATCCAGATGCCTTGCAGGAAATCTTCACTGAGGCCCCAGGCTCCTTCAC15040.15518817598174062No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG14930.15405315607761885No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACCTGGAGCCAGAGATTCGGTCAGGGACCCCTGGGAGGCGATTGATG14570.1503385454823112No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT14470.1493067092058369No Hit
GTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG14330.14786213841877283No Hit
GGTTATCATGTACACTGGTATCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATAATTACATCAATCGC14050.14497299684464465No Hit
ATCATGTACACTGGTATCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATAATTACATCAATCGCCTCC13890.1433220588022857No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13730.14167112075992677No Hit
CTCCTGCGCTGGGGGCAGCTCCAACATCGGGGCAGGTTATCATGTACACTGGTATCAGCAACTTCCAGGAACAGC13570.14002018271756783No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTCCGTAT13380.1380596937922666No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13160.13578965398402304No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13120.1353769194734333No Hit
GTTCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC12870.13279732878224748No Hit
TATTTATTGAGGGCTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12660.13063047260165134No Hit
ATACGGAACCACTCAGGCTGCTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGA12600.13001137083576675No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG12510.12908271818693984No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC12290.12681267837869628No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG12250.12639994386810657No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12200.1258840257298694No Hit
CATGTACACTGGTATCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT12110.12495537308104249No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG11880.1225821496451515No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11600.11969300807102336No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11560.11928027356043361No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11530.11897072267749133No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11350.1171134173798375No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGC11120.11474019394394652No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11100.11453382668865167No Hit
CTCCCAGGTGCACCTGGTGCAGTCTGGGGCTGAGGTGAAGGAGCCTGGGG11050.11401790855041448No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10940.1128828886462927No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGG10930.11277970501864529No Hit
TCCCAGGGGTCCCTGACCGAATCTCTGGCTCCAGGTCTGCCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG10900.11247015413570298No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10820.11164468511452351No Hit
GCCCCCAAACTCCTCATCTATAATTACATCAATCGCCTCCCAGGGGTCCCTGACCGAATCTCTGGCTCCAGG10780.11123195060393376No Hit
CCCCTGGGAGGCGATTGATGTAATTATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGCTGATACCAGTGTA10670.110096930699812No Hit
GCTTGGTCCCTCCGCCGAATACGGAACCACTCAGGCTGCTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCC10610.10947782893392739No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10570.10906509442333767No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10510.10844599265745306No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC10500.10834280902980561No Hit
GTCTGGGGCTGAGGTGAAGGAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCATCTGGATTCACCTTCATCGA10380.10710460549803642No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC10080.1040090966686134No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10040.10359636215802367No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9990.10308044401978649No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9950.10266770950919676No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9910.10225497499860702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)