FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969146
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113031.1662845432989457No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG31620.32626663062118605No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28090.2898428100616419No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCT26810.27663530572277034No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT24540.25321262224680285No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGAACCACTCAGG22760.2348459365255596No Hit
GGCTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA22510.23226634583437375No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG22170.22875810249436102No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGAACCACTCAGGCTGCTGTCGTAG22100.22803581710082896No Hit
GTGCATATAGTGGTCGATGAAGGTGAATCCAGATGCCTTGCAGGAAATCTTCACTGAGGCCCCAGGCTCCTTCAC21960.2265912463137649No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21740.22432120650552134No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT20780.2144155782513677No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20460.2111137021666498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20110.20750227519898962No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC19610.20234309381661794No Hit
GATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGG19370.1998666867530795No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA18060.18634963153126566No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17330.17881722671300299No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC16510.17035616924591343No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15630.16127601001293923No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGGCGGAGGGACCAA15260.15745821578998417No Hit
GGGCAGAGGGTCACCATCTCCTGCGCTGGGGGCAGCTCCAACATCGGGGCAGGTTATCATGTACACTGGTATCA15120.1560136450029201No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGGGCAGCTCCAACATCGGGGCAGGTTATCAT15080.15560091049233038No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG15020.15498180872644574No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC14990.15467225784350347No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT14940.1541563397052663No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC14710.1517831162693753No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTC14680.151473565386433No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG14640.15106083087584327No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT14490.14951307646113174No Hit
GGTTATCATGTACACTGGTATCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATAATTACATCAATCGC14210.14662393488700362No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG14130.14579846586582412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG14100.14548891498288183No Hit
GTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG13920.14363160968522803No Hit
GTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAG13820.14259977340875368No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG13650.1408456517387473No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13530.1396074482069781No Hit
ATCATGTACACTGGTATCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATAATTACATCAATCGCCTCC13490.13919471369638836No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG13390.13816287741991404No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACCTGGAGCCAGAGATTCGGTC12710.1311463907398885No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12640.13042410534635648No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAC12500.12897953455929242No Hit
CTCCTGCGCTGGGGGCAGCTCCAACATCGGGGCAGGTTATCATGTACACTGGTATCAGCAACTTCCAGGAACAGC12480.12877316730399754No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT12410.1280508819104655No Hit
GTCTGGGGCTGAGGTGAAGGAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCATCTGGATTCACCTTCATC12130.12516174033633734No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12120.12505855670868993No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG11940.1232012514110361No Hit
GTTCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11640.1201057425816131No Hit
CATGTACACTGGTATCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATAATTACATCAATCGCCTCCCA11360.11721660100748495No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11280.11639113198630548No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT11240.11597839747571573No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC11080.11432745943335679No Hit
TATTTATTGAGGGCTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGG11040.11391472492276705No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC10850.1119542359974658No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10610.10947782893392739No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10520.10854917628510048No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT10480.10813644177451076No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGGGCA10320.1064855037321518No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG10310.1063823201045044No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGGAACCACTCA10310.1063823201045044No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10230.1055568510833249No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC10190.10514411657273517No Hit
CTCCCAGGTGCACCTGGTGCAGTCTGGGGCTGAGGTGAAGGAGCCTGGGG10140.10462819843449798No Hit
TCCCAGGGGTCCCTGACCGAATCTCTGGCTCCAGGTCTGCCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAG10070.10390591304096596No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10030.10349317853037623No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10000.10318362764743393No Hit
ATACGGAACCACTCAGGCTGCTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGT9930.1024613422539019No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTACGACAGCAGCCTGAGTGGTTCCGTATTCGGCG9920.10235815862625446No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9910.10225497499860702No Hit
GCCCCCAAACTCCTCATCTATAATTACATCAATCGCCTCCCAGGGGTCCCTGACCGAATCTCTGGCTCCAGGT9800.10111995509448524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTA200.0056085414156.8005470
TGGTACA13300.090.7792670
ATATCTA750.003434336562.7202170
CTGTGAA750.003434336562.7202170
GTGGTAT2000.050.0974921
AGTAATA16950.049.95415570
AATGGTA2256.388291E-848.78238770
AGTCATT1407.175432E-444.8001570
TTGATGT11900.043.482570
GGTAGAA21850.041.6221170
AGTGGAA12150.040.00671870
GACAGGT2100.039.7599141
GAGCCAT8750.039.42413370
GTATCAA19700.038.314831
GTCCTAA20650.037.20690570
GTGGTAC11350.037.04557469
TTAACGG558.391655E-636.9476235
GTAGATA1700.001864458836.89424570
CCACCTT5750.035.45055470
TAACCGT601.1474336E-535.29894353
Produced by FastQC (version 0.11.5)