FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031746
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTG37440.3628800111655388No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30980.3002677015466985No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27670.26818616209803575No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA25940.2514184692744144No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24890.24124154588435529No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTC24210.23465077645079313No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22590.2189492375061304No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22470.21778616054726646No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21450.20790000639692327No Hit
GTGTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA21400.20741539099739664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT20640.2000492369245919No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20470.19840154456620138No Hit
GCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGC20100.19481539060970432No Hit
GTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCA19970.19355539057093507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18980.18396000566030787No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGAGA17790.17242615915157414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17730.17184462067214218No Hit
GTATGGTACGGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGGCTCTGGA17450.16913077443479307No Hit
ATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACT17430.1689369282749824No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17290.16758000515630786No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTGGTACC17100.16573846663810668No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC16950.16428462043952677No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16700.16186154344189366No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16700.16186154344189366No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16640.1612800049624617No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16590.16079538956293507No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGG16480.15972923568397648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16260.1575969279260593No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16140.15643385096719542No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16000.15507692784852087No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15900.1541076970494676No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGC15840.15352615857003565No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15830.15342923549013032No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15770.15284769701069836No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15660.15178154313173978No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15610.15129692773221318No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15520.15042462001306522No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT15340.14868000457476938No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAGGTGCAGGTGGTGGAG15270.14800154301543209No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15060.14596615833742027No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15040.1457723121776096No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAACAGTA14740.14286461978044984No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14640.1418953889813966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14620.14170154282158592No Hit
GTTATGGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCC14520.14073231202253267No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14410.13966615814357408No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13810.13385077334925458No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGATGAGGAGCC13710.13288154255020132No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG13520.1310400040320001No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGT13370.12958615783342026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13290.12881077319417764No Hit
GTCTGAGAGCCGAGGACACGGCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACT13240.128326157794651No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTTCCATTCAGTGA13080.12677538851616582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13070.12667846543626046No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG13010.12609692695682853No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12980.12580615771711257No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAG12950.12551538847739657No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12890.12493384999796463No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12750.12357692687929006No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12700.12309231147976343No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12670.12280154224004744No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12620.12231692684052083No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGA12420.12037846524241431No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12390.12008769600269834No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12380.119990772922793No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTT12210.11834308056440249No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12190.11814923440459182No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC11820.1145630804480948No Hit
GTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGGCTGGAG11800.11436923428828412No Hit
ACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCC11760.11398154196866284No Hit
GATCAGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG11540.11184923421074568No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11530.11175231113084035No Hit
GCCCTGAGATTCCCACGTGTTTCCATTCAGTGATCAGCACTGAACACAGA11490.11136461881121903No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT11340.10991077261263917No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGCATCA11240.10894154181358591No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11070.10729384945519536No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG11030.10690615713557405No Hit
GGGCTGGAGTGGGTCTCTGCTATTACTTGGGATGGTGGTAGCACAAGTTA10990.10651846481595276No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10980.10642154173604744No Hit
CCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGC10910.10574308017671015No Hit
GACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGG10830.10496769553746754No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10820.10487077245756223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10800.10467692629775158No Hit
GACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAACAGTATGGTAGCTCA10640.10312615701926636No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG10430.10109077234125452No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAG10390.10070308002163324No Hit
CTACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTC10360.10041231078191726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)