FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031746
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34620.335547702632237No Hit
GTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGT32870.31858616364880504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30210.29280462439398847No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA25860.2506430846351718No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGA21040.20392616012080492No Hit
GTGTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA20750.20111539080355048No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20510.19878923688582267No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTG20430.19801385224658008No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT19800.19190769821254455No Hit
GCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGC19520.18919385197519545No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19310.1871584672971836No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC19140.1855107749387931No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18990.18405692874021318No Hit
GTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT18850.18270000562153862No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18080.17523692846882857No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG17850.1730076976310061No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17500.16961538983431967No Hit
ATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACTTTGACTACTGGGGCCAGGGAACCCA17420.16884000519507708No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC17200.16670769743715994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16890.16370308196009484No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGG16610.1609892357227457No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16550.16040769724331375No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16320.15817846640549127No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16290.1578876971657753No Hit
GTATGGTACGGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGA16270.15769385100596464No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15930.15439846628918358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15920.15430154320927825No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGAGAT15870.15381692780975162No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTG15430.1495523122939173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15180.14712923529628416No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC15050.14586923525751494No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14990.14528769677808298No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG14820.14364000441969244No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14760.1430584659402605No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14700.14247692746082855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14260.13821231194499423No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATT14190.13753385038565694No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAGGTGCAGGTGGTGGAGT13990.13559538878755042No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13730.13307538871001195No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13650.13230000407076933No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTTCCATTCAGT13430.1301676963128522No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT13050.12648461927644983No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12870.12474000383815395No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12860.12464308075824863No Hit
GTCTGAGAGCCGAGGACACGGCCTTATATTACTGTGCGAGACGGGGGTAC12740.12348000379938473No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12690.12299538839985812No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12690.12299538839985812No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGATGAGGAGCCTG12680.12289846531995277No Hit
GTTATGGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCC12640.12251077300033147No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCA12540.12154154220127822No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12530.1214446191213729No Hit
GTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGGCTGGAGT12460.12076615756203561No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12330.11950615752326639No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC12170.11795538824478118No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGT12100.11727692668544389No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11880.11514461892752673No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATA11820.1145630804480948No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC11640.11281846500979893No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11590.11233384961027229No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTA11590.11233384961027229No Hit
GCCCTGAGATTCCCACGTGTTTCCATTCAGTGATCAGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGA11570.11214000345046164No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT11510.11155846497102968No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG11480.11126769573131372No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA11470.11117077265140839No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG11430.11078308033178709No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT11320.10971692645282849No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC11300.10952308029301785No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11180.10836000333415395No Hit
GATCAGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG10980.10642154173604744No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10850.10516154169727822No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTT10820.10487077245756223No Hit
ACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCC10740.10409538781831962No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10740.10409538781831962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10620.10293231085945571No Hit
GGGCTGGAGTGGGTCTCTGCTATTACTTGGGATGGTGGTAGCACAAGTTA10590.10264154161973975No Hit
GACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGG10510.10186615698049714No Hit
CTCCAGCCCCTTCCCTGGAGCTTGGCGGACCCAGCTCATGCCATAATCATCAAAGGTGAATCCAGAGCCTACACA10490.10167231082068649No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10490.10167231082068649No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10450.1012846185010652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTA150.0022969162211.3227870
GTTATTA855.7801277E-574.5845170
CGAGCTT650.001866448473.1501970
TACAATA700.002504154867.9251870
AATGGTA2159.094947E-1266.3455270
GATCGAT252.3534664E-456.1790763
GATAGTC200.00783252950.939773
AAGCGGT11100.047.11926770
TCGAATG405.036655E-543.0740148
GGTATCA6650.041.9260981
GTATCAA17700.039.7639351
GAGCAAA13900.038.7678570
GAGCCAT15200.038.5803170
TTCGAAT451.0152218E-438.24474747
AAATGAA11800.037.6082970
AGCCCAA7650.035.22046370
TATCAAC20100.034.985442
TGAGCTA12400.034.51037670
ATAAACG400.002804390433.961494
GCTATAG502.042105E-433.9598468
Produced by FastQC (version 0.11.5)