FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718341
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA39060.5437528973008641No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30460.42403259733190785No Hit
GTTATTATACTGCTGACAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAC29850.41554080861317955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26720.37196818781052454No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22810.31753721421998743No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21780.303198620153938No Hit
CTTTATTACTGTCAGCAGTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20620.2870503006232416No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT20260.2820387531826806No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18670.2599044186535364No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16360.22774698924327028No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTGTGCACTTTATTACTGTCAGCAGTATAATAAC16290.2267725216853834No Hit
GTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA16100.22412753831397622No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15980.22245702250045593No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15970.22231781284932922No Hit
ATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC15930.22176097424482247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15680.21828073296665512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15150.2109026214569404No Hit
GTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGCCTGGTA15030.20923210564342004No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCAAAAATGAGGAGCC14960.20825763808553321No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGC14900.20742238017877304No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14880.20714396087651968No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14880.20714396087651968No Hit
GGCCAAAACTGTACGGCCAGTTATTATACTGCTGACAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGA14850.20672633192313958No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTGTGCACTTTATTACTGTCAGCAGTA14810.2061694933186328No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14790.2058910740163794No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14590.20310688099384552No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14390.2003226879713117No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTG14250.198373752855538No Hit
GTCTGAAGATTGTGCACTTTATTACTGTCAGCAGTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAG14220.1979561239021579No Hit
GTCAGAGTGTTACCAACAAATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGG13810.19224852820596347No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG12940.18013728855794114No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGA12680.17651783762864712No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12600.17540416041963358No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12330.17164549983921285No Hit
GAGTGTTACCAACAAATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGGTGCATC11780.16398896902724472No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAA11690.16273608216710447No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA11680.1625968725159778No Hit
CAATAATACACGGCCGTGTCCTCGGCTCCCAGGCTGTTCATTTGTAGATACAGTGAGTTCTCGGCGTTGTCCC11390.15855979263330366No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCAAAAA11010.15326982589048932No Hit
GTATTATTGTGTGAGAGGCCGCGCCTTGAAAGCGATCTTTGCAGCACCCGGGGACTACTGGGGCCAGGGAACC10770.14992879426344868No Hit
CAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA10700.14895432670556183No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10430.14519566612514112No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10290.1432467310093674No Hit
GGATACCAGTGGCCCTGGTGGATGCACCAAAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG10170.14157621519584712No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9910.13795676426655307No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9710.1351725712440192No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG9620.13391968438387897No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9450.13155312031472519No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.12960418519895148No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9120.1269592018275443No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA9000.125288686014024No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8930.12431421845613712No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGT8900.12389658950275706No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8880.12361817020050365No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8780.12222607368923671No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8720.12139081578247657No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8300.11554401043515546No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8230.11456954287726859No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8190.11401270427276182No Hit
CCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGC8130.11317744636600165No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7950.11067167264572118No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG7890.10983641473896102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7780.10830510857656739No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC7720.10746985066980724No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA7620.1060777541585403No Hit
CTCCCAGGCTCCTCATTTTTGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTC7540.10496407694952675No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7420.10329356113600643No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7410.10315435148487975No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7400.10301514183375304No Hit
TTATTATACTGCTGACAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC7270.10120541636910603No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7240.10078778741572594No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC7230.10064857776459926No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGCCTGGTA7220.10050936811347258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA100.0024514387206.7780370
TAACTCG150.00252584967.7932422
TGTTAGA6050.051.26728470
GCCGTTA200.00790033450.82723215
CATCTGA12850.050.6887870
GGTATCA6400.042.9123651
TCGTATG2100.042.39533240
CAGTTCG13250.041.35560670
CTTATAC2150.041.002681
CACGAGA2150.040.9912621
ACGAGAC2150.040.9912622
CGTATGC2100.040.81067741
TATACAC2200.040.0540663
ACAGGTA1450.039.7519381
ATGCCGT2250.039.71425644
ATCTCGT2150.039.71335637
CGAGACG2250.039.1721623
ATCTCCG2250.039.155810
TAGCTCC350.001470052238.73634
GTATCAA16150.038.419881
Produced by FastQC (version 0.11.5)