FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718341
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT35620.49586477731328155No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30440.4237541780296544No Hit
GTTATTATACTGCTGACAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT28070.39076149071262817No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA26420.36779189827672376No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20970.29192263841267585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20170.28078586632254043No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT19360.2695098845812783No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT18760.26115730551367666No Hit
CTTTATTACTGTCAGCAGTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18370.2557281291197356No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17070.2376308744732655No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC16410.22844303749890374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15850.2206472970358089No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15300.21299076622384078No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTGTGCACTTTATTACTGTCAGCAGTATAATAACTG14790.2058910740163794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14670.2042205582028591No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA14420.20074031692469177No Hit
GTCTGAAGATTGTGCACTTTATTACTGTCAGCAGTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAA14300.19906980111117142No Hit
ATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC14210.19781691425103118No Hit
GTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGCCTGGTA14200.19767770459990452No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCAAAAATGAGGAGCCTG14170.19726007564652442No Hit
GTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14040.19545035018187742No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT14020.19517193087962403No Hit
GGCCAAAACTGTACGGCCAGTTATTATACTGCTGACAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCT14000.19489351157737064No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT13800.19210931855483676No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13790.19197010890371008No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCT13560.18876828692779613No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGT12790.17804914379104075No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT12780.17790993413991404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12630.17582178937301365No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGT12490.17387285425723994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12400.17261996739709973No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGG12200.16983577437456585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12040.16760841995653875No Hit
GTCAGAGTGTTACCAACAAATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGGT11880.16538106553851165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11540.16064793740020408No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAA11480.15981267949344394No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA11350.1580029540287969No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA11230.1563324382152766No Hit
CAATAATACACGGCCGTGTCCTCGGCTCCCAGGCTGTTCATTTGTAGATACAGTGAGTTCTCGGCGTTGTCCCTG10940.1522953583326025No Hit
GAGTGTTACCAACAAATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGGTGCATC10820.15062484251908215No Hit
CAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA10780.1500680039145754No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10780.1500680039145754No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9470.13183153961697855No Hit
GTATTATTGTGTGAGAGGCCGCGCCTTGAAAGCGATCTTTGCAGCACCCGGGGACTACTGGGGCCAGGGAACC9420.13113549136134509No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9360.13030023345458494No Hit
GGATACCAGTGGCCCTGGTGGATGCACCAAAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAG9060.12612394392078416No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9030.12570631496740406No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG8980.1250102667117706No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8850.12320054124712358No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8760.12194765438698335No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8680.1208339771779698No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8400.11693610694642237No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG8370.11651847799304231No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT8310.11568322008628214No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCA8210.11429112357501522No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8120.11303823671487497No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA7760.10802668927431401No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT7670.10677380241417378No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7660.10663459276304707No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7620.1060777541585403No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA7510.10454644799614667No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7450.10371119008938652No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7410.10315435148487975No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7380.10273672253149967No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC7370.10259751288037297No Hit
CCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAACAAATTAGC7220.10050936811347258No Hit
TTATTATACTGCTGACAGTAATAAAGTGCACAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC7190.1000917391600925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA200.006597744148.5004670
TACGTAT200.006597744148.5004670
GTAGTTT800.005497723455.6876770
ATGGCAA1656.129012E-754.00016870
TAGTTGA200.00797513150.706215
TATGGTT1306.5070856E-445.69244870
AAGCGGT6600.045.00013770
CTGCAAA5350.044.41135470
GGTATCA6850.040.505751
GTATCAA17850.038.8605581
TCCTATA350.001488392838.6386872
GAGCAAA14300.036.34626470
CTGATCA2101.6053284E-435.35725470
GACAGGT2050.034.6624151
GTACCCT807.016024E-834.539947
CGGTACC709.86367E-734.4394745
CGCGGTA709.968098E-734.39312443
TATCAAC20250.034.2262422
TAACGGC701.0580225E-634.1320436
ATCAACG20150.033.8880233
Produced by FastQC (version 0.11.5)