FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545299
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAAAGAGA82070.5310946295830127No Hit
CTATATTACTGTGCGAGAGTTATCGGGAATTTTTGGGCCTACTTTGACTC51780.335080783718879No Hit
ATATTACTGTGCGAGAGTTATCGGGAATTTTTGGGCCTACTTTGACTCCTGGGGCCAGGGAACCCTGGTGAGCG49770.32207359223037096No Hit
CAGTAATATAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAAAGAGAATTGGTTCTTGGACGTGTCTTT48200.3119137461423323No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTCATTACTGGGCCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAG44210.2860935003517119No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT43810.28350500453310334No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC36920.23891816405757071No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA36400.2355531194933796No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT35680.23089382701988415No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34630.22409902549603672No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31690.20507358122926372No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG31340.2028086473879812No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA30890.1998965895920466No Hit
GTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC29980.1940077616047121No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG29530.19109570380877744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT29470.19070742943598618No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG29440.19051329224959054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28740.18598342456702555No Hit
ATATAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAAAGAGAATTGGTTCTTGGACGTGTCTTTTGAGA27580.1784767866930607No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC27530.1781532247157346No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG27180.17588829087445212No Hit
ATGTATTATGATGGGAGCACCTACTACAATCCGTCCCTCTTCAGTCGAGT26900.17407634380142614No Hit
GCCCTATATTACTGTGCGAGAGTTATCGGGAATTTTTGGGCCTACTTTGA26040.16851107779141772No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT25870.16741096706850908No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG25520.16514603322722657No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG25310.16378707292245706No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25130.1626222498040832No Hit
GTTTTGGCCAGGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCA24910.1611985771038485No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC24810.16055145314919636No Hit
GGGCTAGTGAAGCCTTCGGAGACTCTGTCTCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTCATTA24200.15660399702581831No Hit
GGATTGGGACTATGTATTATGATGGGAGCACCTACTACAATCCGTCCCTC23940.15492147474372273No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC23320.15090930622487947No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23230.15032689466569252No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23150.1498091955019708No Hit
GTCTCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTCATTACT22580.1461205889604536No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22280.1441792170964972No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22110.14307910637358853No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22060.14275554439626248No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTCACCAGGGTTCCCTGG21990.14230255762800598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21750.1407494601368408No Hit
GTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGATCCAGGCCCAGTAATGACCACTGCTGATGGAGTAA21570.13958463701846696No Hit
GTAGTAGGTGCTCCCATCATAATACATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGATCCAGGC21400.13848452629555832No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC21350.13816096431823227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT21150.13686671640892797No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG20940.1355077561041585No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTCACCAGGGTTCCC20520.13278983549461948No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20210.13078375123519784No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGGCTAGTGAAGCC20040.1296836405122892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19780.12800111823019364No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC19660.12722456948461108No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG19540.1264480207390285No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT19470.125995033970772No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTCTTCATAACTGG19400.1255420472025155No Hit
CTTCATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAACCCCCTAAACTCCTGATCTATAGGGCGTCTATT19350.1252184852251894No Hit
GGTCATTACTGGGCCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTG19100.12360067533855906No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG19070.12340653815216343No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGGCTAGTGAAGCCTTCGGAGACTCTGTCTCTCACCTGCG19020.12308297617483738No Hit
GTTATGAAGACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGTGGAAGGAGA18430.11926494484238973No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18350.11874724567866801No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA18150.11745299776936373No Hit
CTATAGATCAGGAGTTTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTATGAAGACTCTGAC17760.11492921434622039No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17170.11111118301377274No Hit
GTATTATGATGGGAGCACCTACTACAATCCGTCCCTCTTCAGTCGAGTCACCATCTCAAAAGACACGTCCAAGAA17110.11072290864098146No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17050.11033463426819018No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16930.1095580855226076No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT16930.1095580855226076No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16730.1082638376133033No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16670.10787556324051203No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG16620.10755200126318597No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG16550.10709901449492945No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16400.10612832856295125No Hit
GTCAGGGACCTCCTGTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16310.10554591700376431No Hit
GATCTATAGGGCGTCTATTTTAGAAAGCGGGGTCCCATCAAGGTTTAGCGGCAGTGGCTCTACGACAGAATTCA16260.10522235502643826No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16010.10360454513980791No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA16000.10353983274434268No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC16000.10353983274434268No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC15840.10250443441689924No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15780.10211616004410796No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15750.10192202285771233No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG15720.10172788567131669No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15680.10146903608945583No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAG15670.1014043236939906No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAACTGTGACA15620.10108076171666454No Hit
ACCAGAGACAGCGCAGGTGAGAGACAGAGTCTCCGAAGGCTTCACTAGCCCTGGGCCCGACTCCTGCAGCTGCAC15500.10030421297108198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15480.10017478818015153No Hit
TTACTGGGCCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGACTATGTATTATGATGGGAGCAC15460.10004536338922111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)