FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545299
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAAAGAGAATTGGTTCTTGGACGTGTCTTTTGA73470.47544196948292855No Hit
ATATTACTGTGCGAGAGTTATCGGGAATTTTTGGGCCTACTTTGACTCCTGGGGCCAGGGAACCCTGGTGAGCGT46400.30026551495859377No Hit
CTATATTACTGTGCGAGAGTTATCGGGAATTTTTGGGCCTACTTTGACTCCTGGGGCCAGGGAACCCTGGTGAGC45680.2956062224850983No Hit
CAGTAATATAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAAAGAGAATTGGTTCTTGGACGTGTCTTTT44600.28861728377485524No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTCATTACTGGGCCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTG42980.27813387570949055No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG39850.25787889592887847No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC33610.21749836115858484No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC32620.21109183400752862No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG32580.2108329844256678No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31360.20293807217891166No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG30840.1995730276147205No Hit
GCCCTATATTACTGTGCGAGAGTTATCGGGAATTTTTGGGCCTACTTTGACTCCTGGGGCCAGGGAACCCTGGT29360.1899955930858688No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG29180.18883076996749495No Hit
GTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCA28950.18734238487179505No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC28360.1835243535393474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27980.18106528251166926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27810.17996517178876062No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27730.1794474726250389No Hit
GGATTGGGACTATGTATTATGATGGGAGCACCTACTACAATCCGTCCCTCTTCAGTCGAGTCACCATCTCAAAA27670.17905919825224761No Hit
ATGTATTATGATGGGAGCACCTACTACAATCCGTCCCTCTTCAGTCGAGTCACCATCTCAAAAGACACGTCCAA26490.17142313558735234No Hit
GGTCATTACTGGGCCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGACTATGTATTATGATGG26260.16993475049165244No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25860.16734625467304387No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC25690.16624614395013523No Hit
GGGCTAGTGAAGCCTTCGGAGACTCTGTCTCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTCATTA25140.16268696219954845No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24790.16042202835826594No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG24220.15673342181674874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC23640.15298010287976632No Hit
ATATAGGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAAAGAGAATTGGTTCTTGGACGTGTCTTTTGAGAT23370.15123286820220552No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22840.14780311124254916No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT22610.14631472614684923No Hit
GTTTTGGCCAGGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT22460.14534404021487105No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC22100.14301439397812332No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTCACCAGGGTTCCCTGG22030.14256140720986685No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT21960.14210842044161034No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT21790.14100830971870168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21630.13997291139125825No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT21620.13990819899579304No Hit
GTCTCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTCATTACTGGGCCTGGATCCGGCAGCCCCCAGG21190.13712556599078884No Hit
GTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGATCCAGGCCCAGTAATGACCACTGCTGATGGAGTAA21120.13667257922253234No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC20790.13453707017218028No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20570.13311339747194556No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG20560.13304868507648035No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20170.130524901653337No Hit
GTAGTAGGTGCTCCCATCATAATACATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGATCCAGGC20160.13046018925787178No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19870.12858352978938056No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19680.1273539942755415No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT19610.126901007507285No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG19550.1265127331344937No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC19140.12385952492041993No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGGCTAGTGAAGCCTTCGGAGACTCTGTCTCTCACCTGC18910.12237113982472002No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG18840.12191815305646352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18530.11991206879704186No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTCACCAGGGTTCCCTG18360.11881195807413322No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGGCTAGTGAAGCCTTCGGAGACTCTGTCTCTCACCTGC18030.11667644902378116No Hit
GTTATGAAGACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGTGGAAGGA17960.11622346225552466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17810.11525277632354644No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC17790.11512335153261602No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCGCTGTTCAAGGACCACCT17650.11421737799610303No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG17350.1122760061321466No Hit
CTTCATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAACCCCCTAA17350.1122760061321466No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17140.1109170458273771No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG17030.11020520947725974No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16970.10981693510446847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16890.10929923594074674No Hit
GTATTATGATGGGAGCACCTACTACAATCCGTCCCTCTTCAGTCGAGTCACCATCTCAAAAGACACGTCCAAGAA16830.10891096156795546No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16460.10651660293574253No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA16310.10554591700376431No Hit
ACTACAATCCGTCCCTCTTCAGTCGAGTCACCATCTCAAAAGACACGTCCAAGAACCAATTCTCTTTGAAACT16260.10522235502643826No Hit
CTATAGATCAGGAGTTTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTATGAAGACTCTGACTG16150.1045105186763209No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTC16120.10431638148992524No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT15990.10347512034887746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15630.10114547411212975No Hit
GTCAGGGACCTCCTGTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15620.10108076171666454No Hit
GATCTATAGGGCGTCTATTTTAGAAAGCGGGGTCCCATCAAGGTTTAGCGGCAGTGGCTCTACGACAGAATTCA15490.10023950057561676No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTG15460.10004536338922111No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15460.10004536338922111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATT600.001247013980.9823870
AATGGTA3000.075.5835670
GTTATTA1001.16184645E-464.7859170
TAGGCAA5200.056.06472470
GTAGTTT900.006220348653.98825570
GTGGTAT2950.052.575461
GGTAACA1000.009434248.5894370
ACGGCAT1000.009434248.5894370
TGTGTAT1000.009434248.5894370
GAGCCAT24600.047.4043270
TTGCAAT1406.1217236E-446.27564270
AAGCGGT21950.045.7485970
GAGCAAA24600.042.79556770
AAAGTCA2303.3332635E-642.25167570
GTTTGGT2007.2419694E-540.4911970
GTATCAA27300.039.5216061
CTTTTGA22150.038.33534269
TAAGTCA1700.001591457338.10935670
TCTATTT13650.037.9697670
GACCCAT8300.037.0762770
Produced by FastQC (version 0.11.5)