FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068913
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTACAGCCTCTGGATTCACCTTTGAAGATTATGCCATGCACTGGGTCCGGCAAGCCCCAGGGAAGGGC39860.372902191291527No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36100.33772626958414764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33240.3109701163705559No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGAGG25690.24033761400600423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25300.23668904765869625No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTCTTTTAAAAGGTGTCCA24370.22798862021511573No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT23680.22153346436987856No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23550.22031727558744257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23500.21984951067112102No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATCCCA23340.2183526629388921No Hit
GCCTTGTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTAGTTCAAGTTGCTATTATGGTGACAATTATTTCGA22980.21498475554137708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT22820.21348790780914817No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACCGGTCAGTTGAAGGTAGA22350.20909091759572576No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCAGGAACG22100.2067520930141181No Hit
GCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCAGGAACGTAGCAAC21470.20085825506846677No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC21210.19842587750359478No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21120.19758390065421602No Hit
GTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTAGTTCAAGTTGCTATTATGGTGACAATTATTTCGACTTC20930.19580639397219415No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20870.19524507607260833No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20500.19178361569182897No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20010.18719951951187797No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19860.18579622476291335No Hit
ACCTTCAACTGACCGGTCTGAGAGCTGAGGACACGGCCTTGTATTACTGC19440.18186699946581247No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19430.18177344648254817No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19340.1809314696331694No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGAGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC19330.1808379166499051No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTCTTAAAAACATCATAGATGAGGAGCC19240.17999593980052633No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGAGGCTACTTAGCCTGGTA19230.17990238681726203No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG19110.17877975101809035No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18810.17597316152016113No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18780.17569250257036823No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18270.17092130042388856No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18210.17035998252430273No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18110.16942445269165968No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACCGGTCAGTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTCTGGAGA17810.16661786319373045No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17480.16353061474600833No Hit
GTTTATTACTGTCAGGAACGTAGCAACTGGCCTCCACTCACTTTCGGCGG17380.16259508491336527No Hit
GTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACAGACTCTGCATAACCTA16660.15585927011833517No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16540.15473663431916349No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16490.15426886940284196No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCGAAATAATTGTCAC16400.1534268925534632No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACCGGTCAGTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTC16160.15118162095511983No Hit
GGGTTCCCTGGCCCCAGAAGTCGAAATAATTGTCACCATAATAGCAACTTGAACTAAAACAAAATTCGCTCCCTT16060.15024609112247678No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15800.14781371355760478No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC15640.14631686582537587No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15550.1454748889759971No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15410.14416514721029683No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15390.1439780412437682No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15320.14332317036091805No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15260.14276185246133222No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14960.13995526296340302No Hit
CCATAATAGCAACTTGAACTAAAACAAAATTCGCTCCCTTTTGCGCAGTA14760.13808420329811688No Hit
GTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTC14750.13799065031485255No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCAGGAACGTAGCAAC14600.13658735556588797No Hit
ACTATAGGTTATGCAGAGTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTCTCTACCT14600.13658735556588797No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14530.1359324846830378No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTCTTTTAAAAGG14480.13546471976671629No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA14290.13368721308469445No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14170.13256457728552276No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG14160.13247102430225846No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTCTTAAAAACATCATAGA14100.1319097064026726No Hit
CTCCAGAGACAACGCCAAGAACTCTCTCTACCTTCAACTGACCGGTCTGAGAGCTGAGGACACGGCCTTGTATTA14060.13153549446961538No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC13930.1303193056871794No Hit
GTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13780.1289160109382148No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGTGGAGGCCAGTTGCTACGTTC13770.12882245795495048No Hit
ACCTATAGTACCACTATTCCAACTAATACCTGAGACCCATTCCAGGCCCTTCCCTGGGGCTTGCCGGACCCAG13040.12199309017665609No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12990.12152532526033458No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12980.12143177227707026No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12940.12105756034401303No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT12910.12077690139422012No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT12900.1206833484109558No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12830.12002847752810566No Hit
CCCCAGGGAAGGGCCTGGAATGGGTCTCAGGTATTAGTTGGAATAGTGGT12800.11974781857831272No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12580.11768965294649798No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12530.11722188803017644No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12480.11675412311385491No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12440.11637991118079768No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCC12390.11591214626447616No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGA12350.11553793433141893No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTT12330.11535082836489031No Hit
GTACTATAGGTTATGCAGAGTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTCTCTAC12140.11357332168286849No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG12050.11273134483348972No Hit
CTGCTACTCTGGCTCCCAGATCCCACCGAAGAAATTGTTTTGACACAGTC11970.11198292096737526No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11740.10983120235229622No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11690.10936343743597468No Hit
TTGTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTAGTTCAAGTTGCTATTATGGTGACAATTATTTCGACT11650.10898922550291745No Hit
CCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCTTTGAAGATTATGCC11620.10870856655312454No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11520.10777303672048145No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTTTTTAAGAGGGCCAC11360.10627618898825254No Hit
GCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAGA11140.10421802335643782No Hit
TTCCAGGCCCTTCCCTGGGGCTTGCCGGACCCAGTGCATGGCATAATCTT11140.10421802335643782No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11130.1041244703731735No Hit
GTTTTTAAGAGGGCCACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTC11080.10365670545685196No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10940.10234696369115168No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10920.10215985772462305No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTTTTTAAGAGGGCCACTGGCATCCCAGCCAGAT10740.10047590402586554No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAG10710.10019524507607261No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10710.10019524507607261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGT207.959401E-569.94462662
CCGTTTA250.00297052365.07538669
TGTTAGA6950.059.97810770
TCGCTCG500.004220281359.54969470
AATGGTA1553.274181E-1157.6287370
TCGTATG1900.054.17864640
TAGATCT1850.051.7480834
CTAGATC1850.051.7213733
TATACCT200.007708044351.14676326
ATGTACG200.0077294751.11086322
TTAGGGC200.00773519151.10134
GACCGTA1950.050.70961426
CGTATGC2000.049.77047341
ACCGTAC2000.049.4534527
GGTATCA5250.048.6884081
ACTAGAT2100.047.1736432
ATGCCGT2250.045.8459144
ATCTCGT2100.045.65619737
TACGTAC306.7206234E-445.41712
TACTAGA2200.045.0019431
Produced by FastQC (version 0.11.5)