FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068913
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTACAGCCTCTGGATTCACCTTTGAAGATTATGCCATGCACTGGGTCCGGCAAGCCCCAGGGAAGGGC40090.3750539099066061No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34720.3248159578936733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA32000.29936954644578184No Hit
GCCTTGTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTAGTTCAAGTTGCTATTATGGTGACAATTATTTC23950.22405939491801485No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTCTTTTAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAGTCG23340.2183526629388921No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATCCCA23330.21825910995562783No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGAG22840.2136750137756768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22820.21348790780914817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22440.20993289444510452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22220.20787472881328975No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT21900.20488103334883193No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21690.2029164207002815No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG21240.1987065364533877No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC21170.19805166557053752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20940.19589994695545848No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20780.19440309922322957No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACCGGTCAGTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTCTGGA19920.1863575426624992No Hit
GTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTAGTTCAAGTTGCTATTATGGTGACAATTATTTCGACTTCTG19310.1806508106833765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19280.18037015173358356No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC19190.1795281748842048No Hit
GCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCAGGAACGTAGCAACTGGCCTCCACTCACTTTCGGCGGAG19120.17887330400135465No Hit
ACCTTCAACTGACCGGTCTGAGAGCTGAGGACACGGCCTTGTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTA18930.1770957973193328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18820.17606671450342545No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGAGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT18690.17485052572098944No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGAGGCTACTTAGCCTGGTA18230.17054708849083133No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18200.1702664295410384No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG18180.1700793235745098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18050.16886313479207382No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17760.1661500982774089No Hit
GGGTTCCCTGGCCCCAGAAGTCGAAATAATTGTCACCATAATAGCAACTT17750.16605654529414463No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17560.16427903861212279No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTCTTAAAAACATCATAGATGAGGAGCCTG17360.16240797894683665No Hit
GTTTATTACTGTCAGGAACGTAGCAACTGGCCTCCACTCACTTTCGGCGG17300.16184666104725082No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17210.16100468419787203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16890.15801098873341424No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16870.1578238827668856No Hit
GTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTCTGGAGATGGTGAATCGG16160.15118162095511983No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16010.14977832620615522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15690.14678463074169742No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15530.1452877830094685No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACCGGTCAGTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTCTGGAGAT14860.13901973313075994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14800.1384584152311741No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCGGTTTATTACTGTCAGGAACGTAGCAACTG14770.13817775628138118No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14760.13808420329811688No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACCGGTCAGTTGAAGGTAGAGAGAGTTCTTGGCGTTGTCTCTG14660.1371486734654738No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCGAAATAATTGTCAC14440.13509050783365906No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT14420.13490340186713043No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14370.13443563695080893No Hit
GTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTCTCTACCTTCAACTGACCGGTCTGA14180.13265813026878706No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTCTTAAAAACATCATA14170.13256457728552276No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG14090.1318161534194083No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA13900.1300386467373865No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGTGGAGGCCAGTTGCTACGTTC13800.12910311690474344No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13690.12807403408883603No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG13680.12798048110557175No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13470.12601586845702129No Hit
CCATAATAGCAACTTGAACTAAAACAAAATTCGCTCCCTTTTGCGCAGTA13470.12601586845702129No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13450.12582876249049268No Hit
ACTATAGGTTATGCAGAGTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTCTCTACCTT13340.1247996796745853No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT13250.12395770282520653No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGT13010.12171243122686318No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGTGGAGGCCA12940.12105756034401303No Hit
CTCCAGAGACAACGCCAAGAACTCTCTCTACCTTCAACTGACCGGTCTGAGAGCTGAGGACACGGCCTTGTATTA12900.1206833484109558No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT12830.12002847752810566No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC12810.11984137156157704No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12800.11974781857831272No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12680.11862518277914104No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT12620.11806386487955521No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12590.1177832059297623No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT12540.11731544101344075No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC12530.11722188803017644No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTG12490.11684767609711921No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA12360.11563148731468323No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12360.11563148731468323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12320.115257275381626No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12310.11516372239836171No Hit
GTACTATAGGTTATGCAGAGTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCTCTCTAC12280.11488306344856877No Hit
TTGTATTACTGCGCAAAAGGGAGCGAATTTTGTTTTAGTTCAAGTTGCTATTATGGTGACAATTATTTCGACTT12220.11432174554898294No Hit
GTAATAAACCGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12140.11357332168286849No Hit
ACCTATAGTACCACTATTCCAACTAATACCTGAGACCCATTCCAGGCCCTTCCCTGGGGCTTGCCGGACCCAGT12130.11347976869960417No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC11830.11067317920167498No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11340.10608908302172394No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG11200.10477934125602364No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11150.1043115763397021No Hit
GTTTTTAAGAGGGCCACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTC11140.10421802335643782No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTTTTTAAGAGGGCCACTG11060.10346959949032336No Hit
CCCCAGGGAAGGGCCTGGAATGGGTCTCAGGTATTAGTTGGAATAGTGGT11060.10346959949032336No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTT11040.10328249352379473No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10910.10206630474135873No Hit
GAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTCTTTTAAAAGGTGTCCAGTGT10880.10178564579156582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACA506.928541E-493.8860570
AATGGTA1801.8189894E-1278.23836570
TCCCTTT7900.071.30585570
CATCGAA1153.3563483E-668.0333770
GGTCTTA700.002634648567.0614670
TAAGTCA1051.7529145E-459.61018870
ATGCGGG200.00707750352.2627260
TAGGCAA1501.6215286E-552.15891370
AAACTCA950.00882446849.41370870
GTGGTAT1600.048.9191361
TGGTATC1600.048.8642042
AAGCGGT11100.047.92981370
TTACTTT1356.0612825E-446.3634870
GAGCCAT13350.044.54019570
GGTATCA6000.041.4040221
GAGCAAA16350.041.15290570
AACCTAT13850.039.5428670
GTATCAA14850.039.416031
ACATTTT3252.6744601E-838.51735370
ACTCAGT14100.035.5124570
Produced by FastQC (version 0.11.5)