FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439582
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA60160.4178990845953895No Hit
ATCTATTACTGTGCGAGAGATCAGAGGCCCTGGTTCGGGGAGTTTACGTTTGACTTCTGGGGCCAGGGAACCC51800.35982667190892914No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGT49710.345308568737314No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTGCATCATAGCTTCTGAGGCTGTCTCCTTGGCATGTGA44820.3113403751922433No Hit
CCCCTGTACTTGTCATCTATGATAAAAATAACCGGCCCTCAGGGATCCCA38430.26695249037567853No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36670.2547267192837921No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG35790.2486138337378489No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGATAAAAATAACCGGCCCTCAGGGATCCCAGACCGATTCTC29170.20262826292632166No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTC29040.20172522301612553No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATGATAACCTCTGCTGTCCCGGGAG27660.19213910704635095No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27140.18852694740556633No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTGCATCATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTG26430.18359496020372582No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT26290.18262245568505303No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG26130.181511021949427No Hit
ATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT25920.1800522651714178No Hit
GACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATGATAACCT25690.17845457917645538No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCCATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG25510.1772042162238761No Hit
CTATTACTGTAACTCCCGGGACAGCAGAGGTTATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACG25220.17518974257805392No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT24900.17296687510680184No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24860.17268901667289532No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24810.1723416936305122No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24040.1669929187778119No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAG23840.16560362660827935No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23790.1652563035658962No Hit
CCCCATGATAACCTCTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGG23560.16365861757093378No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23400.16254718383530775No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAG23400.16254718383530775No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA23330.16206093157597135No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23220.16129682088272845No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC22040.15309999708248645No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21630.15025194813494472No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21620.1501824835264681No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21380.14851533292302904No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20960.1455978193670107No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20890.1451115671076743No Hit
GACTATTACTGTAACTCCCGGGACAGCAGAGGTTATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTA20830.14469477945681455No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20260.1407352967736468No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19990.13885975234477785No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATGATGCAAACTGGTA19620.13628956183114266No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19560.1358727741802829No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA19280.1339277651429373No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG19140.13295526062426455No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19110.13274686679883466No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG18850.13094078697844236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG18660.12962095941738644No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG18590.12913470715805003No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18380.12767595038004087No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18300.12712023351222787No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18260.12684237507832136No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17940.12461950760706927No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA17870.12413325534773288No Hit
GGGCTGGAGTGGGTGGCAATTATATGGTATGATGGAAGAAATGAATACTA17870.12413325534773288No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17540.12184092326800418No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17130.11899287432046246No Hit
ATGATAACCTCTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTT16820.11683947145768703No Hit
CAGTAATAGATAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACAGGGTATTCTTGGAATTGTCTC16810.11677000684921039No Hit
GGTTATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCAC16300.1132273118169024No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16130.11204641347279974No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16060.11156016121346335No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGAGGTTATCATGGGGTGT16020.11128230277955684No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15990.11107390895412697No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAATACCCTGTATCTGCAAATGAA15990.11107390895412697No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15980.11100444434565035No Hit
GTGTCAGGTGGAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGA15540.10794800157267874No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15470.10746174931334233No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15380.1068365678370527No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC15310.1063503155777163No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15230.10579459870990329No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATGGCTAC15100.10489155879970714No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15060.10461370036580063No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14980.1040579834979876No Hit
GGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAATTATATGGTATGATGGAAGAAA14800.10280762054540832No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14760.1025297621115018No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14750.10246029750302517No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14440.10030689464024974No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG14440.10030689464024974No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14400.10002903620634322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3550.097.1801770
TCTGGAG18800.079.1365870
TCTCGCG11300.046.7491470
GGTATCA11700.046.435661
CGTATGC2150.043.0857241
TATACAC4700.043.0684663
TCGTATG1600.042.81818440
CTTATAC4600.041.8146671
AATGGTA2853.6379788E-1241.61058470
GTATCAA30300.040.4273831
TTATACA4850.040.3438382
TACACAT4750.039.7741055
CACGAGA4800.039.3611621
CTCTGGA18900.039.14639769
ACGAGAC4950.038.16972422
ACACATC5100.037.0445146
ATACACA5400.036.860764
TCTCGTA1950.036.7727938
TCTCCGA5200.036.32959411
GGTACTA601.1136775E-535.4549760
Produced by FastQC (version 0.11.5)