FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439582
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACAGGGTATTCTTGGAATTGTCTCTGGA53470.3714272615245259No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC49640.3448223164779776No Hit
ATCTATTACTGTGCGAGAGATCAGAGGCCCTGGTTCGGGGAGTTTACGTTTGACTTCTGGGGCCAGGGAACCCTG48590.3375285325879318No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGTTTGCATCATAGCTTCTGAGGCTGTCTCCTTGGCATGT43660.3032824806089545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39430.27389895122334124No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37160.2581304850991468No Hit
CCCCTGTACTTGTCATCTATGATAAAAATAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGCCTCCAGCTCAG35050.2434734527105785No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33870.23527662891033646No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGATAAAAATAACCGGCCCTCAGGGATCCCAGACCGATTCTCT27320.18977731035814563No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT27010.1876239074953702No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTC26910.1869292614106039No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGG26400.18338656637829592No Hit
ATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCC25750.17887136682731516No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25530.17734314544082935No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCC24800.17227222902203557No Hit
GTCCTGGCTTCTGCTGGTACCAGTTTGCATCATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT24730.17178597676269916No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24030.16692345416933527No Hit
CTATTACTGTAACTCCCGGGACAGCAGAGGTTATCATGGGGTGTTCGGCG23990.16664559573542875No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA23890.16595094965066248No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA23710.16470058669808318No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAA23690.16456165748112994No Hit
CCCCATGATAACCTCTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCA23660.16435326365570005No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATGGCTACTGAAGGTGAATCCAGACGCTGCACA23530.16345022374550391No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCCATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGG23390.16247771922683113No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT23330.16206093157597135No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23270.1616441439251116No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22550.15664269211479442No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22460.1560175106385048No Hit
GACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCATGATAACCT21920.1522664217807669No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21750.15108552343666426No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG21130.14677871771111337No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG21070.14636193006025358No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG20880.1450421024991977No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20620.14323602267880536No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAG20550.14274977041946899No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG19740.13712313713286217No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19460.1351781280955166No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19000.13198275610559176No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATGATGCAAACTGGTA18690.12982935324281633No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC18440.12809273803090063No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18370.12760648577156425No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG18340.12739809194613438No Hit
GACTATTACTGTAACTCCCGGGACAGCAGAGGTTATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTA18340.12739809194613438No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGGCA18180.12628665821050833No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18050.12538361830031217No Hit
GGGCTGGAGTGGGTGGCAATTATATGGTATGATGGAAGAAATGAATACTA17830.12385539691382637No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17740.12323021543753673No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17640.12253556935277045No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC17000.11808983441026631No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16990.11802036980178969No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT16970.11788144058483643No Hit
ATGATAACCTCTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAA16820.11683947145768703No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG16620.11545017928815447No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGGCAGA16610.11538071467967785No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16250.11287998877451927No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16220.11267159494908938No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG16130.11204641347279974No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15850.11010140443545417No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC15480.10753121392181897No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT15460.10739228470486573No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15330.10648924479466956No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG15310.1063503155777163No Hit
GGTTATCATGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACT14840.10308547897931483No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT14790.10273815593693168No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAATACCCTGTATCTGCAAATGAA14770.10259922671997843No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC14630.10162672220130566No Hit
CAGTAATAGATAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACAGGGTATTCTTGGAATTGTCTCTG14630.10162672220130566No Hit
GTATTCATTTCTTCCATCATACCATATAATTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGT14410.10009850081481986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3050.069.51897470
ATGGTTT1153.230273E-452.67897870
CTAGTAC200.00806033950.5726551
GGTAGAA24850.045.1004170
GGTATCA11750.044.4752431
TGTGGTT36750.042.4477970
GAGCCAT15350.042.42630470
GTATCAA31000.039.5880551
AAGCGGT11800.038.5047670
TGGTATC3200.036.848992
CTCTGGA20500.036.57861369
CGATTCA14400.035.7595170
GTAGTTT2151.6455885E-435.2214170
AGTGGAA16400.035.0925570
TTAACGG1550.035.0795435
TGATGGT25900.034.50066470
TGACAGT34300.034.44099470
GCATTAT400.00290667133.7151031
TATCAAC36400.033.320282
CTCTTAA9150.033.10427570
Produced by FastQC (version 0.11.5)