FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735051
Sequences flagged as poor quality0
Sequence length18-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC10650.14488790573715293No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT9380.12761019303422483No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAA9150.12448115845022999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3350.066.7690470
TCGTATG2000.044.65469740
CTCGTAT1950.043.90929839
CGTATGC2100.040.9756141
GTATCAA30800.039.8819161
GGTATCA11850.035.023861
TCTCGTA2550.034.84796538
ACGAGAC2850.032.8854222
GACCGGA2851.03937055E-832.10664770
TCAACGC38600.031.8813674
ATCAACG38650.031.8401243
TATACAC3300.031.4140683
CAACGCA39150.031.2626485
TCTCCGA3000.031.20927411
AACGCAG39400.031.0642816
TATCAAC40450.030.3405882
CGCAGAG41550.029.6158148
TGGTATC4800.029.2606852
ATCTCCG3200.029.25869610
ACGCAGA42050.029.1841437
Produced by FastQC (version 0.11.5)